BNCR_DANRE
ID BNCR_DANRE Reviewed; 125 AA.
AC P0DPQ9;
DT 07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT 07-NOV-2018, sequence version 1.
DT 03-AUG-2022, entry version 12.
DE RecName: Full=Protein Bouncer {ECO:0000303|PubMed:30190407};
DE Flags: Precursor;
GN Name=bncr {ECO:0000303|PubMed:30190407};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, TISSUE SPECIFICITY,
RP DISRUPTION PHENOTYPE, AND MUTAGENESIS OF ASN-32 AND ASN-84.
RX PubMed=30190407; DOI=10.1126/science.aat7113;
RA Herberg S., Gert K.R., Schleiffer A., Pauli A.;
RT "The Ly6/uPAR protein Bouncer is necessary and sufficient for species-
RT specific fertilization.";
RL Science 361:1029-1033(2018).
CC -!- FUNCTION: Oocyte-expressed fertilization factor that mediates sperm-egg
CC binding and is essential for sperm entry into the egg
CC (PubMed:30190407). Necessary and sufficient to mediate species-specific
CC gamete recognition and fertilization, which is essential for vertebrate
CC species performing external fertilization (PubMed:30190407). External
CC fertilization cannot guarantee that only conspecific sperm reaches the
CC egg by precopulatory mate choice: proteins such as Bouncer can
CC therefore support the selection of conspecific sperm (PubMed:30190407).
CC {ECO:0000269|PubMed:30190407}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:30190407};
CC Lipid-anchor, GPI-anchor {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Highly expressed in oocytes (PubMed:30190407). Not
CC expressed in testis (PubMed:30190407). {ECO:0000269|PubMed:30190407}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:30190407}.
CC -!- DISRUPTION PHENOTYPE: Females are almost sterile: only 7 of 3024 eggs
CC derived from mutant females develop into cleavage-stage embryos, as
CC opposed to the majority of eggs from wild-type (PubMed:30190407). Both
CC male and female fishes develop normally and are overtly healthy
CC (PubMed:30190407). Males are fertile (PubMed:30190407).
CC {ECO:0000269|PubMed:30190407}.
CC -!- MISCELLANEOUS: Was named bouncer (bncr) in reference to the colloquial
CC name of a security guard at a bar. {ECO:0000305|PubMed:30190407}.
CC -!- SIMILARITY: Belongs to the SPACA4/bouncer family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=Silent walls - Issue 210 of
CC January 2019;
CC URL="https://web.expasy.org/spotlight/back_issues/210/";
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DR EMBL; CABZ01080228; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_005173770.1; XM_005173713.1.
DR AlphaFoldDB; P0DPQ9; -.
DR GeneID; 101885477; -.
DR CTD; 101885477; -.
DR PRO; PR:P0DPQ9; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IDA:UniProtKB.
DR GO; GO:0007338; P:single fertilization; IDA:UniProtKB.
DR GO; GO:0035036; P:sperm-egg recognition; IDA:UniProtKB.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR016054; LY6_UPA_recep-like.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR046354; SPACA4/Bouncer.
DR PANTHER; PTHR47613; PTHR47613; 1.
DR Pfam; PF00021; UPAR_LY6; 1.
DR SMART; SM00134; LU; 1.
DR SUPFAM; SSF57302; SSF57302; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; Fertilization; Glycoprotein; GPI-anchor;
KW Lipoprotein; Membrane; Reference proteome; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..98
FT /note="Protein Bouncer"
FT /evidence="ECO:0000255"
FT /id="PRO_0000445656"
FT PROPEP 99..125
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000445657"
FT DOMAIN 23..98
FT /note="UPAR/Ly6"
FT /evidence="ECO:0000255"
FT LIPID 98
FT /note="GPI-anchor amidated asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 32
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 84
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 23..48
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT DISULFID 26..35
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT DISULFID 42..66
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT DISULFID 72..91
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT DISULFID 92..97
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT MUTAGEN 32
FT /note="N->A: Glycosylation-defective mutant. Does not
FT affect ability to promote fertilization and mediate sperm-
FT egg binding; when associated with A-84."
FT /evidence="ECO:0000269|PubMed:30190407"
FT MUTAGEN 84
FT /note="N->A: Glycosylation-defective mutant. Does not
FT affect ability to promote fertilization and mediate sperm-
FT egg binding; when associated with A-32."
FT /evidence="ECO:0000269|PubMed:30190407"
SQ SEQUENCE 125 AA; 13368 MW; 20AAA63014CCC15F CRC64;
MGCVLLFLLL VCVPVVLPQG LRCLFCPVTS LNSSCAPVVT ECPVQELCYT ADGRFGRSSV
LFRKGCMLRA DCSRSRHQMI RGNNISFSFS CCGGHYCNSQ PRAEPGGRLL LLLLPAAALT
AAGAL
