ID   BNCR_ORYLA              Reviewed;         131 AA.
AC   H2LID1;
DT   07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 1.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=Protein Bouncer {ECO:0000303|PubMed:30190407};
DE   Flags: Precursor;
GN   Name=bncr {ECO:0000303|PubMed:30190407};
OS   Oryzias latipes (Japanese rice fish) (Japanese killifish).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Ovalentaria; Atherinomorphae; Beloniformes; Adrianichthyidae; Oryziinae;
OC   Oryzias.
OX   NCBI_TaxID=8090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Hd-rR;
RX   PubMed=17554307; DOI=10.1038/nature05846;
RA   Kasahara M., Naruse K., Sasaki S., Nakatani Y., Qu W., Ahsan B., Yamada T.,
RA   Nagayasu Y., Doi K., Kasai Y., Jindo T., Kobayashi D., Shimada A.,
RA   Toyoda A., Kuroki Y., Fujiyama A., Sasaki T., Shimizu A., Asakawa S.,
RA   Shimizu N., Hashimoto S., Yang J., Lee Y., Matsushima K., Sugano S.,
RA   Sakaizumi M., Narita T., Ohishi K., Haga S., Ohta F., Nomoto H., Nogata K.,
RA   Morishita T., Endo T., Shin-I T., Takeda H., Morishita S., Kohara Y.;
RT   "The medaka draft genome and insights into vertebrate genome evolution.";
RL   Nature 447:714-719(2007).
RN   [2]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=30190407; DOI=10.1126/science.aat7113;
RA   Herberg S., Gert K.R., Schleiffer A., Pauli A.;
RT   "The Ly6/uPAR protein Bouncer is necessary and sufficient for species-
RT   specific fertilization.";
RL   Science 361:1029-1033(2018).
CC   -!- FUNCTION: Oocyte-expressed fertilization factor that mediates sperm-egg
CC       binding and is essential for sperm entry into the egg
CC       (PubMed:30190407). Necessary and sufficient to mediate species-specific
CC       gamete recognition and fertilization, which is essential for vertebrate
CC       species performing external fertilization (PubMed:30190407). External
CC       fertilization cannot guarantee that only conspecific sperm reaches the
CC       egg by precopulatory mate choice: proteins such as Bouncer can
CC       therefore support the selection of conspecific sperm (PubMed:30190407).
CC       {ECO:0000269|PubMed:30190407}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P0DPQ9};
CC       Lipid-anchor, GPI-anchor {ECO:0000250|UniProtKB:P0DPQ9}.
CC   -!- TISSUE SPECIFICITY: Expressed in oocytes (PubMed:30190407). Not
CC       expressed in testis (PubMed:30190407). {ECO:0000269|PubMed:30190407}.
CC   -!- MISCELLANEOUS: Zebrafish oocytes expressing O.latipes protein Bouncer
CC       can be fertilized by O.latipes, but not zebrafish, sperm.
CC       {ECO:0000269|PubMed:30190407}.
CC   -!- SIMILARITY: Belongs to the SPACA4/bouncer family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=Silent walls - Issue 210 of
CC       January 2019;
CC       URL="https://web.expasy.org/spotlight/back_issues/210/";
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DR   EMBL; BAAF04034626; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BAAF04034627; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; H2LID1; -.
DR   SMR; H2LID1; -.
DR   HOGENOM; CLU_1926927_0_0_1; -.
DR   OMA; SCCDWPY; -.
DR   Proteomes; UP000001038; Unplaced.
DR   Proteomes; UP000265180; Chromosome 9.
DR   Proteomes; UP000265200; Chromosome 9.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IDA:UniProtKB.
DR   GO; GO:0007338; P:single fertilization; IDA:UniProtKB.
DR   GO; GO:0035036; P:sperm-egg recognition; IDA:UniProtKB.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR046354; SPACA4/Bouncer.
DR   PANTHER; PTHR47613; PTHR47613; 1.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Disulfide bond; Fertilization; Glycoprotein; GPI-anchor;
KW   Lipoprotein; Membrane; Reference proteome; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..106
FT                   /note="Protein Bouncer"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5003565015"
FT   PROPEP          107..131
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000445658"
FT   DOMAIN          31..106
FT                   /note="UPAR/Ly6"
FT                   /evidence="ECO:0000255"
FT   LIPID           106
FT                   /note="GPI-anchor amidated asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        65
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   DISULFID        31..56
FT                   /evidence="ECO:0000250|UniProtKB:P13987"
FT   DISULFID        50..74
FT                   /evidence="ECO:0000250|UniProtKB:P13987"
FT   DISULFID        80..99
FT                   /evidence="ECO:0000250|UniProtKB:P13987"
FT   DISULFID        100..105
FT                   /evidence="ECO:0000250|UniProtKB:P13987"
SQ   SEQUENCE   131 AA;  14641 MW;  CCF2946D90550DA6 CRC64;
     MGSLRTRQLF HAALLWLCLP LPLLLCENLH CYYSPVLEKE ITFELVVTEC PPNEMCFKGL
     GRYGNYTALS ARGCMLEKDC SQVHSLRLLG TVYTMSYSCC DWPYCNRAVA LEPLTAMLVA
     AAVVACSFCL T