SYR_NEIG2
ID SYR_NEIG2 Reviewed; 572 AA.
AC B4RL22;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Arginine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00123};
DE EC=6.1.1.19 {ECO:0000255|HAMAP-Rule:MF_00123};
DE AltName: Full=Arginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00123};
DE Short=ArgRS {ECO:0000255|HAMAP-Rule:MF_00123};
GN Name=argS {ECO:0000255|HAMAP-Rule:MF_00123}; OrderedLocusNames=NGK_0832;
OS Neisseria gonorrhoeae (strain NCCP11945).
OC Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC Neisseria.
OX NCBI_TaxID=521006;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCCP11945;
RX PubMed=18586945; DOI=10.1128/jb.00566-08;
RA Chung G.T., Yoo J.S., Oh H.B., Lee Y.S., Cha S.H., Kim S.J., Yoo C.K.;
RT "Complete genome sequence of Neisseria gonorrhoeae NCCP11945.";
RL J. Bacteriol. 190:6035-6036(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC EC=6.1.1.19; Evidence={ECO:0000255|HAMAP-Rule:MF_00123};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00123}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00123}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00123}.
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DR EMBL; CP001050; ACF29513.1; -; Genomic_DNA.
DR RefSeq; WP_003688336.1; NC_011035.1.
DR PDB; 6AO8; X-ray; 1.70 A; A=1-572.
DR PDBsum; 6AO8; -.
DR AlphaFoldDB; B4RL22; -.
DR SMR; B4RL22; -.
DR EnsemblBacteria; ACF29513; ACF29513; NGK_0832.
DR GeneID; 66753286; -.
DR KEGG; ngk:NGK_0832; -.
DR HOGENOM; CLU_006406_5_1_4; -.
DR OMA; NKPLHLG; -.
DR OrthoDB; 1146366at2; -.
DR Proteomes; UP000002564; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR CDD; cd00671; ArgRS_core; 1.
DR Gene3D; 3.30.1360.70; -; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR001278; Arg-tRNA-ligase.
DR InterPro; IPR005148; Arg-tRNA-synth_N.
DR InterPro; IPR036695; Arg-tRNA-synth_N_sf.
DR InterPro; IPR035684; ArgRS_core.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR11956; PTHR11956; 1.
DR Pfam; PF03485; Arg_tRNA_synt_N; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF00750; tRNA-synt_1d; 1.
DR PRINTS; PR01038; TRNASYNTHARG.
DR SMART; SM01016; Arg_tRNA_synt_N; 1.
DR SMART; SM00836; DALR_1; 1.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF55190; SSF55190; 1.
DR TIGRFAMs; TIGR00456; argS; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..572
FT /note="Arginine--tRNA ligase"
FT /id="PRO_1000095384"
FT MOTIF 122..132
FT /note="'HIGH' region"
FT HELIX 3..17
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 27..29
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 37..40
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 43..47
FT /evidence="ECO:0007829|PDB:6AO8"
FT TURN 48..51
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 54..64
FT /evidence="ECO:0007829|PDB:6AO8"
FT TURN 65..67
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 69..77
FT /evidence="ECO:0007829|PDB:6AO8"
FT TURN 78..80
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 81..86
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 88..100
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 114..118
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 130..148
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 152..157
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 165..179
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 188..201
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 203..218
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 221..245
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 251..253
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 257..263
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 264..273
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 276..280
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 283..288
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 289..291
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 299..304
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 312..325
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 330..337
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 338..340
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 341..353
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 362..368
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 376..378
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 389..407
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 413..433
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 445..448
FT /evidence="ECO:0007829|PDB:6AO8"
FT STRAND 451..455
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 456..473
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 487..496
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 499..509
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 513..532
FT /evidence="ECO:0007829|PDB:6AO8"
FT HELIX 540..563
FT /evidence="ECO:0007829|PDB:6AO8"
SQ SEQUENCE 572 AA; 62619 MW; E63B0879504F9020 CRC64;
MNLHQTVEHE AAAAFAAAGI AGSPVVLQPT KNAEHGDFQI NGVMGAAKKA KQNPRELAQK
VADALAGNAV IESAEVAGPG FINLRLRHEF LAQNIHAALN DARFGVAKTA QPQTVVIDYS
SPNLAKEMHV GHLRSSIIGD SISRVLEFTG NTVIRQNHVG DWGTQFGMLV AYLVEQQKDN
AAFELADLEQ FYRAAKVRFD EDPAFADTAR EYVVKLQGGD ETVLALWKQF VDISLSHAQA
VYDTLGLKLR PEDVAGESKY NDDLQPVADD LVQKGLAVED DGAKVVFLDE FKNKEGEPAA
FIVQKQGGGF LYASTDLACL RYRIGRLKAG RLLYVVDHRQ ALHFEQLFTT SRKAGYLPEN
AKAEFIGFGT MMGKDGKPFK TRSGDTVKLV DLLTEAVERA TALVKEKNPE LGADEAAKIG
KTVGIGAVKY ADLSKNRTSD YVFDWDAMLS FEGNTAPYLQ YAYTRVQSVF RKAGEWDATA
PTVLTEPLEK QLAAELLKFE NVLQSVADTA YPHYLAAYLY QAATLFSRFY EACPILKAEG
ASRNSRLQLA KLTGNTLKQG LDLLGIDVLD VM
