ID   SYR_NITMU               Reviewed;         581 AA.
AC   Q2Y676;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2006, sequence version 2.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Arginine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00123};
DE            EC=6.1.1.19 {ECO:0000255|HAMAP-Rule:MF_00123};
DE   AltName: Full=Arginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00123};
DE            Short=ArgRS {ECO:0000255|HAMAP-Rule:MF_00123};
GN   Name=argS {ECO:0000255|HAMAP-Rule:MF_00123}; OrderedLocusNames=Nmul_A2456;
OS   Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosospira.
OX   NCBI_TaxID=323848;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25196 / NCIMB 11849 / C 71;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M.,
RA   Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Lykidis A., Richardson P.;
RT   "Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC
RT   25196.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC         tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC         COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC         EC=6.1.1.19; Evidence={ECO:0000255|HAMAP-Rule:MF_00123};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00123}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00123}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00123}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABB75745.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000103; ABB75745.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_049783179.1; NZ_FNVK01000014.1.
DR   AlphaFoldDB; Q2Y676; -.
DR   SMR; Q2Y676; -.
DR   STRING; 323848.Nmul_A2456; -.
DR   PRIDE; Q2Y676; -.
DR   EnsemblBacteria; ABB75745; ABB75745; Nmul_A2456.
DR   KEGG; nmu:Nmul_A2456; -.
DR   eggNOG; COG0018; Bacteria.
DR   HOGENOM; CLU_006406_0_1_4; -.
DR   OrthoDB; 1146366at2; -.
DR   Proteomes; UP000002718; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd00671; ArgRS_core; 1.
DR   Gene3D; 3.30.1360.70; -; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR001278; Arg-tRNA-ligase.
DR   InterPro; IPR005148; Arg-tRNA-synth_N.
DR   InterPro; IPR036695; Arg-tRNA-synth_N_sf.
DR   InterPro; IPR035684; ArgRS_core.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   PANTHER; PTHR11956; PTHR11956; 1.
DR   Pfam; PF03485; Arg_tRNA_synt_N; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF00750; tRNA-synt_1d; 2.
DR   PRINTS; PR01038; TRNASYNTHARG.
DR   SMART; SM01016; Arg_tRNA_synt_N; 1.
DR   SMART; SM00836; DALR_1; 1.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF55190; SSF55190; 1.
DR   TIGRFAMs; TIGR00456; argS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..581
FT                   /note="Arginine--tRNA ligase"
FT                   /id="PRO_0000242057"
FT   MOTIF           131..141
FT                   /note="'HIGH' region"
SQ   SEQUENCE   581 AA;  64698 MW;  95CCF9C2BAF3D7D9 CRC64;
     MIPPVQPDFK SHFTDILRNA LNERGLADLN LDIEFARPRQ SSHGDYSCNL AMQLAKPLRQ
     KPRDIAQSLA TAFSASPYLE KVEIAGAGFI NLFLTTSAKQ QFSRYVLESG EKFGHSSMGA
     GEKIQVEFVS ANPTGPLHVG HGRGAAFGAS LANVLAAAGY SVTREYYIND AGRQMDILAL
     STWLRYLELN GVASAFPPNA YQGEYVRDMA RLIHKAHAGR YVHEPELLFD RVAGAEADTE
     AALDGLIANA KKLLGQDYAY IHNFVLNEQL GDCRNDLMEF GVTFDIWFSE QSLFDSGGVA
     QAVHLLEEGN YLYQQDGAKW FRSSHFGDEK DRVVQRENGQ FTYFASDIAY HLNKFSRGFD
     RVIDIWGADH HGYISRVKGA MQALALDPEK LEIALVQFAV LYRDGKKVPM STRAGEFVTL
     RELRQEVGTD AARFFYVLRK SDQHLDFDLD LAKSQSTDNP VYYVQYAHAR VCSVLEQWGE
     DPGMLVTADT SALTGAAELS LLQKLIDYPE TVEAAAREFS PHLIAFYLKE LAGEFHSYYN
     STRFLVPEMT VRLARLALVA AVRQVLNNGL KLLGVSAPAK M