SYR_PSEAE
ID SYR_PSEAE Reviewed; 587 AA.
AC Q9HUC8;
DT 10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Arginine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00123};
DE EC=6.1.1.19 {ECO:0000255|HAMAP-Rule:MF_00123};
DE AltName: Full=Arginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00123};
DE Short=ArgRS {ECO:0000255|HAMAP-Rule:MF_00123};
GN Name=argS {ECO:0000255|HAMAP-Rule:MF_00123}; OrderedLocusNames=PA5051;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC EC=6.1.1.19; Evidence={ECO:0000255|HAMAP-Rule:MF_00123};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00123}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00123}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00123}.
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DR EMBL; AE004091; AAG08436.1; -; Genomic_DNA.
DR PIR; B83015; B83015.
DR RefSeq; NP_253738.1; NC_002516.2.
DR RefSeq; WP_003110892.1; NZ_QZGE01000002.1.
DR AlphaFoldDB; Q9HUC8; -.
DR SMR; Q9HUC8; -.
DR STRING; 287.DR97_2406; -.
DR PaxDb; Q9HUC8; -.
DR PRIDE; Q9HUC8; -.
DR EnsemblBacteria; AAG08436; AAG08436; PA5051.
DR GeneID; 881194; -.
DR KEGG; pae:PA5051; -.
DR PATRIC; fig|208964.12.peg.5295; -.
DR PseudoCAP; PA5051; -.
DR HOGENOM; CLU_006406_5_1_6; -.
DR InParanoid; Q9HUC8; -.
DR OMA; NKPLHLG; -.
DR PhylomeDB; Q9HUC8; -.
DR BioCyc; PAER208964:G1FZ6-5167-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IBA:GO_Central.
DR CDD; cd00671; ArgRS_core; 1.
DR Gene3D; 3.30.1360.70; -; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR001278; Arg-tRNA-ligase.
DR InterPro; IPR005148; Arg-tRNA-synth_N.
DR InterPro; IPR036695; Arg-tRNA-synth_N_sf.
DR InterPro; IPR035684; ArgRS_core.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR11956; PTHR11956; 1.
DR Pfam; PF03485; Arg_tRNA_synt_N; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF00750; tRNA-synt_1d; 1.
DR PRINTS; PR01038; TRNASYNTHARG.
DR SMART; SM01016; Arg_tRNA_synt_N; 1.
DR SMART; SM00836; DALR_1; 1.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF55190; SSF55190; 1.
DR TIGRFAMs; TIGR00456; argS; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..587
FT /note="Arginine--tRNA ligase"
FT /id="PRO_0000151592"
FT MOTIF 127..137
FT /note="'HIGH' region"
SQ SEQUENCE 587 AA; 65199 MW; A31AF6885B56D5FC CRC64;
MKDTIRQLIQ QALDQLTADG TLPAGLTPDI QVENTKDRSH GDFASNIAMM LAKPAGMKPR
DLAARLVEAI PAHEQLAKVE IAGPGFLNFF QDHVWLAASL DRALADERLG VRKAGPAQRV
VIDLSSPNLA KEMHVGHLRS TIIGDAVARV LEFLGDTVIR QNHVGDWGTQ FGMLLAYLEE
QPVDAEAELH DLEVFYRAAK KRFDESPEFA DRARELVVKL QAGDPDCLRL WTRFNEISLS
HCQKVYDRLG VKLSMADVMG ESAYNDDLAQ VVADLTAKGL LTEDNGALCV FLEEFKNAEG
NPLPVIVQKA GGGYLYATTD LAAMRYRHNV LHADRVLYFV DQRQALHFQQ VFEVARRAGF
VPAGMELEHM GFGTMNGADG RPFKTRDGGT VKLIDLLEEA ESRAYALVKE RNEQRAERGE
EPFDEVQLRE IGRVVGIDSV KYADLSKHRT SDYSFNFELM LSFEGNTAPY LLYACTRVAS
VFRKLGQGRE QLGGKIVLEQ PQELALAAQL AQFGDLINNV ALKGVPHLLC AYLYELAGLF
SSFYEHCPIL TAEDPAQKDS RLRLAALTGR TLEQGLELLG LKTLERM
