SYR_PYRAB
ID SYR_PYRAB Reviewed; 625 AA.
AC Q9V0V2; G8ZJF0;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=Arginine--tRNA ligase;
DE EC=6.1.1.19;
DE AltName: Full=Arginyl-tRNA synthetase;
DE Short=ArgRS;
GN Name=argS; OrderedLocusNames=PYRAB06880; ORFNames=PAB0469;
OS Pyrococcus abyssi (strain GE5 / Orsay).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=272844;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GE5 / Orsay;
RX PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA Weissenbach J., Zivanovic Y., Forterre P.;
RT "An integrated analysis of the genome of the hyperthermophilic archaeon
RT Pyrococcus abyssi.";
RL Mol. Microbiol. 47:1495-1512(2003).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=GE5 / Orsay;
RX PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA Gao J., Wang J.;
RT "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT Pyrococcus furiosus DSM 3638.";
RL Curr. Microbiol. 64:118-129(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC EC=6.1.1.19;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000305}.
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DR EMBL; AJ248285; CAB49601.1; -; Genomic_DNA.
DR EMBL; HE613800; CCE70077.1; -; Genomic_DNA.
DR PIR; H75110; H75110.
DR RefSeq; WP_010867806.1; NC_000868.1.
DR AlphaFoldDB; Q9V0V2; -.
DR SMR; Q9V0V2; -.
DR STRING; 272844.PAB0469; -.
DR EnsemblBacteria; CAB49601; CAB49601; PAB0469.
DR GeneID; 1495590; -.
DR KEGG; pab:PAB0469; -.
DR PATRIC; fig|272844.11.peg.722; -.
DR eggNOG; arCOG00487; Archaea.
DR HOGENOM; CLU_006406_6_1_2; -.
DR OMA; NKPLHLG; -.
DR OrthoDB; 7046at2157; -.
DR PhylomeDB; Q9V0V2; -.
DR Proteomes; UP000000810; Chromosome.
DR Proteomes; UP000009139; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR CDD; cd00671; ArgRS_core; 1.
DR Gene3D; 3.30.1360.70; -; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR001278; Arg-tRNA-ligase.
DR InterPro; IPR005148; Arg-tRNA-synth_N.
DR InterPro; IPR036695; Arg-tRNA-synth_N_sf.
DR InterPro; IPR035684; ArgRS_core.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR11956; PTHR11956; 1.
DR Pfam; PF03485; Arg_tRNA_synt_N; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF00750; tRNA-synt_1d; 2.
DR PRINTS; PR01038; TRNASYNTHARG.
DR SMART; SM01016; Arg_tRNA_synt_N; 1.
DR SMART; SM00836; DALR_1; 1.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF55190; SSF55190; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..625
FT /note="Arginine--tRNA ligase"
FT /id="PRO_0000151650"
FT MOTIF 128..138
FT /note="'HIGH' region"
SQ SEQUENCE 625 AA; 71691 MW; F1B1E7D79AFEC891 CRC64;
MLSRVKEGAR EKIIKVIESL APGWKGEIEF KDTPSPELGD FGTPVAFKLA RVMKKPPFQI
AELIVEELKK ELPEGIREVR AINGYVNFFV DYGYLARQLI VEVLEKGDRF GSSDIGRGKK
VIVEHTSVNP TKPLHMGHAR NAILGDSMAR ILRFLGYEVE VQNYIDDLGI QFAQVYWGYL
KLREKFEKIM KELKEKGIKE DPIDHVLGLL YVEVNRVLEE NPEIEAEIRD IMKKLESGEL
NGRELAESVV RAQMVTTYRL GIKYDLLVWE SDIVSRKLFE IAVKLLERNE NFYTPNEGKY
KGAFVMDLSK LFPDMKNPYL VLRRSDGTAT YTGKDIAYHL WKFGKIDVDL LYKPWDEHTW
TTAPDGESVK GKFGGADIVI NVIGAEQRHP QMAIKHALKL LGFEDAAENL HHLAYEHVER
PEGKFSGRKG TWVGFTVDEV INEAIKRARE LIEEKSPGLS DEEKEEIAEK VGVGAIRYNM
VKYSPDKKII FRWEDVLNFE GESAPYIQYA HARCSSILRK AGNPEWEALL KEANFKELTE
RERELIILLS KFPEVVEQAG RDVKPHLIAW YANELASLFN KFYMDHPVIK AEKGIREARL
LLVMAVRQVL RNSLWLLGIE APDRM