ID   SYR_PYRFU               Reviewed;         629 AA.
AC   Q8U149;
DT   10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Arginine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00123};
DE            EC=6.1.1.19 {ECO:0000255|HAMAP-Rule:MF_00123};
DE   AltName: Full=Arginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00123};
DE            Short=ArgRS {ECO:0000255|HAMAP-Rule:MF_00123};
GN   Name=argS {ECO:0000255|HAMAP-Rule:MF_00123}; OrderedLocusNames=PF1380;
OS   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=186497;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA   Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA   DiRuggiero J., Robb F.T.;
RT   "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT   horikoshii inferred from complete genomic sequences.";
RL   Genetics 152:1299-1305(1999).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC         tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC         COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC         EC=6.1.1.19; Evidence={ECO:0000255|HAMAP-Rule:MF_00123};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00123}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00123}.
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DR   EMBL; AE009950; AAL81504.1; -; Genomic_DNA.
DR   RefSeq; WP_011012527.1; NZ_CP023154.1.
DR   AlphaFoldDB; Q8U149; -.
DR   SMR; Q8U149; -.
DR   STRING; 186497.PF1380; -.
DR   EnsemblBacteria; AAL81504; AAL81504; PF1380.
DR   GeneID; 41713188; -.
DR   KEGG; pfu:PF1380; -.
DR   PATRIC; fig|186497.12.peg.1443; -.
DR   eggNOG; arCOG00487; Archaea.
DR   HOGENOM; CLU_006406_6_1_2; -.
DR   OMA; NKPLHLG; -.
DR   OrthoDB; 7046at2157; -.
DR   PhylomeDB; Q8U149; -.
DR   Proteomes; UP000001013; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd00671; ArgRS_core; 1.
DR   Gene3D; 3.30.1360.70; -; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR001278; Arg-tRNA-ligase.
DR   InterPro; IPR005148; Arg-tRNA-synth_N.
DR   InterPro; IPR036695; Arg-tRNA-synth_N_sf.
DR   InterPro; IPR035684; ArgRS_core.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   PANTHER; PTHR11956; PTHR11956; 1.
DR   Pfam; PF03485; Arg_tRNA_synt_N; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF00750; tRNA-synt_1d; 2.
DR   PRINTS; PR01038; TRNASYNTHARG.
DR   SMART; SM01016; Arg_tRNA_synt_N; 1.
DR   SMART; SM00836; DALR_1; 1.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF55190; SSF55190; 1.
DR   TIGRFAMs; TIGR00456; argS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..629
FT                   /note="Arginine--tRNA ligase"
FT                   /id="PRO_0000151652"
FT   MOTIF           128..138
FT                   /note="'HIGH' region"
SQ   SEQUENCE   629 AA;  72281 MW;  9AEA216208AB71DF CRC64;
     MMETIKSEIK RTIEGIVREM APDWSEDIQF VDTPSPELGD FGTPVAFQLA RLLRKSPLII
     AQEIAEKFNK NKPKEVKKAI AVNGYVNFFL DYPQISKLVI EAILGYGTEY GRSEIGKGKK
     VIVEHTSVNP TKPLHMGHAR NAILGDTVAR ILRFLGYQVE VQNYIDDLGV QFAQVYWGYL
     NLKRKFDELM KELKEKIPKN NPIDHVLGLL YVEVNKKIEE SSEVEKEIRE LMKKLEEREL
     NGRKLAEEVV KAQMETLYSL NIYYDLLVWE SDIVSTRLFE KTIKLLEKNE NFYTPKEGKY
     KGAFVMDLSK LFPDMKNPYL VLRRSDGTAT YTGKDIAYHL WKFGKIDIDL MYKKWDEHTW
     TTAPDGEPIP GKFGAGDIVI NVIGAEQRHP QLAIKYALEL LGYKDAAENF HHLAYEHVES
     PEGKFSGRKG TWVGFTVDEV IAEAINKAKS LIEEKNPNLT EEEKEEIAKK VAVGAIRYTL
     IKYSPEKKIV FRWEDVLNFE GESAPYIQYA HARCSSILRK AEELGISTDW KSLLKVANFN
     QITEKERELI MLLSRFPEIV QQAGTDLKPH LIAWYANEVA STFNKFYMDH PVIKAEEGVR
     EARLLLVMAT RQVLRNSLWL MGIEAPDKM