SYR_PYRHO
ID SYR_PYRHO Reviewed; 629 AA.
AC O59147;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 25-MAY-2022, entry version 135.
DE RecName: Full=Arginine--tRNA ligase;
DE EC=6.1.1.19;
DE AltName: Full=Arginyl-tRNA synthetase;
DE Short=ArgRS;
GN Name=argS; OrderedLocusNames=PH1478;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC EC=6.1.1.19;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000305}.
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DR EMBL; BA000001; BAA30585.1; -; Genomic_DNA.
DR PIR; A71023; A71023.
DR RefSeq; WP_010885558.1; NC_000961.1.
DR PDB; 2ZUE; X-ray; 2.00 A; A=1-629.
DR PDB; 2ZUF; X-ray; 2.30 A; A=1-629.
DR PDBsum; 2ZUE; -.
DR PDBsum; 2ZUF; -.
DR AlphaFoldDB; O59147; -.
DR SMR; O59147; -.
DR STRING; 70601.3257902; -.
DR EnsemblBacteria; BAA30585; BAA30585; BAA30585.
DR GeneID; 1443796; -.
DR KEGG; pho:PH1478; -.
DR eggNOG; arCOG00487; Archaea.
DR OMA; NKPLHLG; -.
DR OrthoDB; 7046at2157; -.
DR BRENDA; 6.1.1.19; 5244.
DR EvolutionaryTrace; O59147; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR CDD; cd00671; ArgRS_core; 1.
DR Gene3D; 3.30.1360.70; -; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR001278; Arg-tRNA-ligase.
DR InterPro; IPR005148; Arg-tRNA-synth_N.
DR InterPro; IPR036695; Arg-tRNA-synth_N_sf.
DR InterPro; IPR035684; ArgRS_core.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR11956; PTHR11956; 1.
DR Pfam; PF03485; Arg_tRNA_synt_N; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF00750; tRNA-synt_1d; 2.
DR PRINTS; PR01038; TRNASYNTHARG.
DR SMART; SM01016; Arg_tRNA_synt_N; 1.
DR SMART; SM00836; DALR_1; 1.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF55190; SSF55190; 1.
DR TIGRFAMs; TIGR00456; argS; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..629
FT /note="Arginine--tRNA ligase"
FT /id="PRO_0000151653"
FT MOTIF 128..138
FT /note="'HIGH' region"
FT HELIX 6..20
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 36..38
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 40..43
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 45..53
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 57..68
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 76..82
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 85..90
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 92..106
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 107..109
FT /evidence="ECO:0007829|PDB:2ZUE"
FT TURN 115..118
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 120..124
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 136..154
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 158..165
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 170..181
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 183..196
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 203..220
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 222..224
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 225..237
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 238..240
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 243..260
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 265..269
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 270..275
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 278..287
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 296..298
FT /evidence="ECO:0007829|PDB:2ZUE"
FT TURN 299..302
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 304..307
FT /evidence="ECO:0007829|PDB:2ZUE"
FT TURN 309..311
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 313..316
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 319..323
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 331..342
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 351..354
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 356..358
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 360..362
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 377..384
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 385..387
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 388..400
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 404..408
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 410..415
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 418..420
FT /evidence="ECO:0007829|PDB:2ZUE"
FT TURN 427..434
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 437..455
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 461..481
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 493..496
FT /evidence="ECO:0007829|PDB:2ZUE"
FT STRAND 500..503
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 504..523
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 530..536
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 544..565
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 570..589
FT /evidence="ECO:0007829|PDB:2ZUE"
FT HELIX 599..620
FT /evidence="ECO:0007829|PDB:2ZUE"
SQ SEQUENCE 629 AA; 72340 MW; 34CE2D841DBAD48A CRC64;
MLMEIRESVK ERIEEIIKEI APQWEGEIEL KETPDPKLGD FGTPIAFKLA KLLKRPPIEI
AEKIVEKLKL NLPEGIKDVK AVNGYINVFI DYPHFARILI NDILAKGDRF GSSEIGKGKK
VIVEHTSVNP TKPLHMGHAR NAILGDVMAR ILRFLGYEVE VQNYIDDLGI QFAQVYWGYL
RLKEEFERIM NELRERGLKD NPIDHALGLL YVEVNRRLED NPELENEIRD IMKKLESGEL
YGRKLAEEVV RAQMVTTYKL GVKYDLLVWE SDIVRRKLFE IALELLSKNE NFYIPSDGKY
RGAFVMDLRK LFPDMKNPIL VLRRSDGTAT YTGKDIAYHL WKFGKIDVDL LYKEWDSTTW
TTAPDGKSMP NKFGNANIVI NVIGAEQKHP QLAIKYALQL LGFEDAAANL YHLAYEHVER
PEGKFSGRKG TWVGFTVDEV IQEAVKRARE LIEEKNPALS DEEKAEVAEK VGIGAIRYNL
IKYSPDKKII FRWEDVLNFE GESAPYIQYA HARCSSILRK AEEEGIKVDP ETLFKNADFT
KLSERERELV IMLSKFPRIV EQAGKDVKPH LIAWFANELA SLFNKFYMDH PVLKAEEGVR
EARLLLVMAV EQVLKNALYL MGIEAPERM
