SYR_RALPJ
ID SYR_RALPJ Reviewed; 596 AA.
AC B2UDW0;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Arginine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00123};
DE EC=6.1.1.19 {ECO:0000255|HAMAP-Rule:MF_00123};
DE AltName: Full=Arginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00123};
DE Short=ArgRS {ECO:0000255|HAMAP-Rule:MF_00123};
GN Name=argS {ECO:0000255|HAMAP-Rule:MF_00123}; OrderedLocusNames=Rpic_0137;
OS Ralstonia pickettii (strain 12J).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=402626;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=12J;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C.,
RA Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.,
RA Kyrpides N., Mikhailova N., Marsh T., Richardson P.;
RT "Complete sequence of chromosome 1 of Ralstonia pickettii 12J.";
RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC EC=6.1.1.19; Evidence={ECO:0000255|HAMAP-Rule:MF_00123};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00123}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00123}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00123}.
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DR EMBL; CP001068; ACD25300.1; -; Genomic_DNA.
DR RefSeq; WP_009239247.1; NC_010682.1.
DR AlphaFoldDB; B2UDW0; -.
DR SMR; B2UDW0; -.
DR STRING; 402626.Rpic_0137; -.
DR EnsemblBacteria; ACD25300; ACD25300; Rpic_0137.
DR GeneID; 61388251; -.
DR KEGG; rpi:Rpic_0137; -.
DR eggNOG; COG0018; Bacteria.
DR HOGENOM; CLU_006406_0_1_4; -.
DR OMA; NKPLHLG; -.
DR OrthoDB; 1146366at2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR CDD; cd00671; ArgRS_core; 1.
DR Gene3D; 3.30.1360.70; -; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR001278; Arg-tRNA-ligase.
DR InterPro; IPR005148; Arg-tRNA-synth_N.
DR InterPro; IPR036695; Arg-tRNA-synth_N_sf.
DR InterPro; IPR035684; ArgRS_core.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR11956; PTHR11956; 1.
DR Pfam; PF03485; Arg_tRNA_synt_N; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF00750; tRNA-synt_1d; 1.
DR PRINTS; PR01038; TRNASYNTHARG.
DR SMART; SM01016; Arg_tRNA_synt_N; 1.
DR SMART; SM00836; DALR_1; 1.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF55190; SSF55190; 1.
DR TIGRFAMs; TIGR00456; argS; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..596
FT /note="Arginine--tRNA ligase"
FT /id="PRO_1000095395"
FT MOTIF 132..142
FT /note="'HIGH' region"
SQ SEQUENCE 596 AA; 64626 MW; 21B543764C5A9675 CRC64;
MLPSHKQTIA QLLSDAVGSL LPEGQARPEI VLERPKQAAH GDIACNVALQ LAKPLGTNPR
ELAGKIADAI RADARGQRLV AAVEIAGPGF INLRLSAAAR ADVIAAVFAE GDRYGAANTH
DGAPVLVEFV SANPTGPLHV GHGRQAALGD ALASLLDWQG HKVHREFYYN DAGVQIHNLA
VSVQARARGY KPGDANWPEA AYNGDYIADI AADYLAGKTV RASDGEPVTG ERDVENIEAI
RRFAVTYLRN EQDIDLQAFG VKFDRYYLES SLYTEGKVQQ TVDALVAAGK TYEQEGALWL
RTTDDGDDKD RVMRKSDGAY TYFVPDVAYH TTKWGRGFTQ VINVQGSDHH GTIARVRAGL
QGLNIGIPKG YPDYVLHKMV TVMKDGAEVK ISKRAGSYVT VRDLIEWSNG DAESEAGVDT
IRACVEAGEP NWPARFTRGR DAVRFFLLSR KADTEFVFDV DLALKQSDEN PVYYVQYAHA
RICSVFERAG VDAASLTSAD LAAVTGPDVS PQATALVQRL AAFPDMLADA ARELAPHAVA
FYLRDLAGDF HAFYNADRVL VDDEAVKRAR LALLAATRQV LRNGLAVIGV SAPQKM