ID   BNM2A_BRANA             Reviewed;         282 AA.
AC   O65009;
DT   17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=BURP domain-containing protein BNM2A {ECO:0000303|PubMed:19714473};
DE   AltName: Full=Brassica napus microspore-derived embryo protein 2 {ECO:0000303|PubMed:9790599};
DE            Short=BNM2 {ECO:0000303|PubMed:9790599};
DE   Flags: Precursor;
GN   Name=BNM2A {ECO:0000303|PubMed:19714473};
GN   OrderedLocusNames=BnaA08g02780D {ECO:0000312|EMBL:CDY16489.1};
GN   ORFNames=GSBRNA2T00091082001 {ECO:0000312|EMBL:CDY16489.1};
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708 {ECO:0000312|EMBL:AAC15700.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND DOMAIN.
RX   PubMed=9790599; DOI=10.1007/s004380050832;
RA   Hattori J., Boutilier K.A., van Lookeren Campagne M.M., Miki B.L.;
RT   "A conserved BURP domain defines a novel group of plant proteins with
RT   unusual primary structures.";
RL   Mol. Gen. Genet. 259:424-428(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL
RP   STAGE, AND SUBCELLULAR LOCATION.
RX   PubMed=19714473; DOI=10.1007/s11103-009-9541-7;
RA   Teerawanichpan P., Xia Q., Caldwell S.J., Datla R., Selvaraj G.;
RT   "Protein storage vacuoles of Brassica napus zygotic embryos accumulate a
RT   BURP domain protein and perturbation of its production distorts the PSV.";
RL   Plant Mol. Biol. 71:331-343(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Darmor-bzh;
RX   PubMed=25146293; DOI=10.1126/science.1253435;
RA   Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA   Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA   Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA   Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA   Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA   Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA   Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA   Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA   Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA   Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA   Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA   Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA   Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT   "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT   Brassica napus oilseed genome.";
RL   Science 345:950-953(2014).
CC   -!- SUBCELLULAR LOCATION: Protein storage vacuole
CC       {ECO:0000269|PubMed:19714473}.
CC   -!- TISSUE SPECIFICITY: Expressed in the radicle and cotyledon of
CC       germinating seeds 2 days post-imbibition (DPI), in stems and roots of
CC       30-DPI young plants and in floral buds, but not in fully open flowers
CC       or leaves. Expressed in the embryo and seed coat tissues of developing
CC       seeds. The protein accumulates only in seeds and only long after
CC       transcript accumulation becomes evident. {ECO:0000269|PubMed:19714473}.
CC   -!- DEVELOPMENTAL STAGE: Peak of expression in seeds between 3 and 28 days
CC       post-anthesis. {ECO:0000269|PubMed:19714473}.
CC   -!- DOMAIN: The BURP domain located at the C-terminus has not been
CC       identified in non-plant proteins. {ECO:0000269|PubMed:9790599}.
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DR   EMBL; AF049028; AAC15700.1; -; mRNA.
DR   EMBL; FJ203999; ACO54860.1; -; mRNA.
DR   EMBL; FJ204000; ACO54861.1; -; Genomic_DNA.
DR   EMBL; LK032064; CDY16489.1; -; Genomic_DNA.
DR   PIR; T07844; T07844.
DR   RefSeq; NP_001303011.1; NM_001316082.1.
DR   AlphaFoldDB; O65009; -.
DR   EnsemblPlants; CDY16489; CDY16489; GSBRNA2T00091082001.
DR   GeneID; 106439079; -.
DR   Gramene; CDY16489; CDY16489; GSBRNA2T00091082001.
DR   KEGG; bna:106439079; -.
DR   OMA; LWVVEAY; -.
DR   Proteomes; UP000028999; Unassembled WGS sequence.
DR   GO; GO:0000326; C:protein storage vacuole; IEA:UniProtKB-SubCell.
DR   InterPro; IPR044816; BURP.
DR   InterPro; IPR004873; BURP_dom.
DR   PANTHER; PTHR31236; PTHR31236; 1.
DR   Pfam; PF03181; BURP; 1.
DR   SMART; SM01045; BURP; 1.
DR   PROSITE; PS51277; BURP; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; Signal; Vacuole.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..282
FT                   /note="BURP domain-containing protein BNM2A"
FT                   /id="PRO_5005928898"
FT   DOMAIN          60..282
FT                   /note="BURP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00604"
SQ   SEQUENCE   282 AA;  32089 MW;  375CEE9717C089FE CRC64;
     MASLRFSVTF PALLSLLLLS LWVVEAYTSR KLISNNEQEG QNISHLFKDG EFEDPTMYMF
     FKISDLKLGT KLPIYFNKND LRKVPPLLTR QEADLIPFSE SNLDFLLNHF SISKDSPQGK
     AMKETLKRCD FKAIEGEYKF CGTSLESMLD LAKKTIASNA DLKVMTTKVM VPDQNRISYA
     LHNYTFAEVP KELDGIKVLG CHRMPYPYVV YYCHGHKSGT KVFEVNLMSD DGIQLVVGPA
     VCHMDTSMWN ADHVAFKVLK IEPRSAPVCH FFPLDNIVWV SK