SYR_VIBCH
ID SYR_VIBCH Reviewed; 577 AA.
AC Q9KQC6;
DT 10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-MAY-2003, sequence version 3.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Arginine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00123};
DE EC=6.1.1.19 {ECO:0000255|HAMAP-Rule:MF_00123};
DE AltName: Full=Arginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00123};
DE Short=ArgRS {ECO:0000255|HAMAP-Rule:MF_00123};
GN Name=argS {ECO:0000255|HAMAP-Rule:MF_00123}; OrderedLocusNames=VC_2074;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC EC=6.1.1.19; Evidence={ECO:0000255|HAMAP-Rule:MF_00123};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00123}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00123}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00123}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF95220.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE003852; AAF95220.1; ALT_INIT; Genomic_DNA.
DR PIR; G82121; G82121.
DR RefSeq; NP_231706.1; NC_002505.1.
DR RefSeq; WP_001025289.1; NZ_LT906614.1.
DR AlphaFoldDB; Q9KQC6; -.
DR SMR; Q9KQC6; -.
DR STRING; 243277.VC_2074; -.
DR PRIDE; Q9KQC6; -.
DR DNASU; 2613330; -.
DR EnsemblBacteria; AAF95220; AAF95220; VC_2074.
DR KEGG; vch:VC_2074; -.
DR PATRIC; fig|243277.26.peg.1982; -.
DR eggNOG; COG0018; Bacteria.
DR HOGENOM; CLU_006406_5_1_6; -.
DR OMA; NKPLHLG; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IBA:GO_Central.
DR CDD; cd00671; ArgRS_core; 1.
DR Gene3D; 3.30.1360.70; -; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR001278; Arg-tRNA-ligase.
DR InterPro; IPR005148; Arg-tRNA-synth_N.
DR InterPro; IPR036695; Arg-tRNA-synth_N_sf.
DR InterPro; IPR035684; ArgRS_core.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR11956; PTHR11956; 1.
DR Pfam; PF03485; Arg_tRNA_synt_N; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF00750; tRNA-synt_1d; 1.
DR PRINTS; PR01038; TRNASYNTHARG.
DR SMART; SM01016; Arg_tRNA_synt_N; 1.
DR SMART; SM00836; DALR_1; 1.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF55190; SSF55190; 1.
DR TIGRFAMs; TIGR00456; argS; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..577
FT /note="Arginine--tRNA ligase"
FT /id="PRO_0000151632"
FT MOTIF 122..132
FT /note="'HIGH' region"
SQ SEQUENCE 577 AA; 63877 MW; 6E6B73420F9DBDE0 CRC64;
MNIQALINDR VSQAIEAAGA PAGTPALVRQ SAKAQFGDYQ ANGIMGAAKQ LGTNPREFAQ
KVLDVLNLEG IASKTEIAGP GFINIFLSEE FLAAQAEAAL ADARLGVAQE APKTIVADYS
APNVAKEMHV GHLRSTIIGD AVVRTLEFLG HKVIRANHIG DWGTQFGMLI ANLERVQKAS
GEVSMELSDL EAFYRESKKL YDEDEQFAET ARNYVVKLQG GDPFCLEMWK KLVDVTMIQN
QRNYDRLNVS LTRENVMGES MYNDMLPQIV SDLKAKGLAV EDDGAQVVFL EEFKNKDGEP
MGVIIQKRDG GFLYTTTDIA CAKYRYETLG ADRVLYFIDS RQHQHLMQAW TIVRKAGYIP
ENVSLEHHAF GMMLGKDGRP FKTRAGGTVR LADLLDEAQE RAKALIESKN PELSAEEKAN
IANTVAMAAV KYADLSKHRT TDYVFDWDNM LAFEGNTAPY MQYAYTRVAS VFAKAGVDMN
ELTGHIQITE EKEKALIAKL LQFEEAVQSV AREGQPHIMC SYLFELAGIF SSFYEACPIL
VAEQESIKQS RLKLAALTAK TIKQGLALLG IDTLERM
