ID   BNPL2_CROOA             Reviewed;          32 AA.
AC   B3EWY2;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2013, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Natriuretic peptide Coa_NP2 {ECO:0000303|PubMed:22617223};
OS   Crotalus oreganus abyssus (Grand Canyon rattlesnake) (Crotalus abyssus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Crotalus.
OX   NCBI_TaxID=128077;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom {ECO:0000269|PubMed:22617223};
RX   PubMed=22617223; DOI=10.1016/j.peptides.2012.05.005;
RA   Da Silva S.L., Dias-Junior C.A., Baldasso P.A., Damico D.C., Carvalho B.M.,
RA   Garanto A., Acosta G., Oliveira E., Albericio F., Soares A.M.,
RA   Marangoni S., Resende R.R.;
RT   "Vascular effects and electrolyte homeostasis of the natriuretic peptide
RT   isolated from Crotalus oreganus abyssus (North American Grand Canyon
RT   rattlesnake) venom.";
RL   Peptides 36:206-212(2012).
CC   -!- FUNCTION: Snake venom natriuretic peptide that exhibits hypotensive and
CC       vasorelaxant effects. Produces a dose-dependent hypotension in rats,
CC       followed by significant increases in concentrations of markers of
CC       nitric oxide (NO) formation measured in the plasma and vasorelaxation
CC       in a thoracic aortic ring bath. The peptide may exert its hypotensive
CC       action, at least in part, through stimulation of NO production. The
CC       vasorelaxant effect is endothelium-dependent and does not appear to be
CC       mediated by the natriuretic peptide receptor-A, as its action is not
CC       modified by isatin (a potent NPR1 antagonist). May act by activating
CC       the natriuretic peptide receptor-B (NPR2).
CC       {ECO:0000269|PubMed:22617223}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22617223}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:22617223}.
CC   -!- MASS SPECTROMETRY: Mass=3419.88; Method=MALDI; Note=Average mass.;
CC       Evidence={ECO:0000269|PubMed:22617223};
CC   -!- MISCELLANEOUS: The hypotensive effect is not mediated by the
CC       natriuretic peptide receptor-A (NPR1). {ECO:0000305|PubMed:22617223}.
CC   -!- SIMILARITY: Belongs to the natriuretic peptide family. Snake NP
CC       subfamily. {ECO:0000255}.
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DR   AlphaFoldDB; B3EWY2; -.
DR   SMR; B3EWY2; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0097746; P:blood vessel diameter maintenance; IDA:UniProtKB.
DR   GO; GO:0008217; P:regulation of blood pressure; IDA:UniProtKB.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
DR   InterPro; IPR000663; Natr_peptide.
DR   InterPro; IPR030480; Natr_peptide_CS.
DR   InterPro; IPR002408; Natriuretic_peptide_brain.
DR   Pfam; PF00212; ANP; 1.
DR   PRINTS; PR00712; BNATPEPTIDE.
DR   PRINTS; PR00710; NATPEPTIDES.
DR   SMART; SM00183; NAT_PEP; 1.
DR   PROSITE; PS00263; NATRIURETIC_PEPTIDE; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Hypotensive agent; Secreted;
KW   Toxin; Vasoactive; Vasodilator.
FT   PEPTIDE         1..32
FT                   /note="Natriuretic peptide Coa_NP2"
FT                   /evidence="ECO:0000269|PubMed:22617223"
FT                   /id="PRO_0000421178"
FT   DISULFID        8..24
FT                   /evidence="ECO:0000250|UniProtKB:P83228"
SQ   SEQUENCE   32 AA;  3419 MW;  2D00A2E7C146B55A CRC64;
     SYGISSGCFG LKLDRIGTMS GLGCWRLLQD SP