BNZB_PSEPU
ID BNZB_PSEPU Reviewed; 187 AA.
AC P0C619; P08085; P13451;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=Benzene 1,2-dioxygenase subunit beta;
DE EC=1.14.12.3;
DE AltName: Full=Benzene 1,2-dioxygenase P2 subunit;
DE AltName: Full=Toluene 2,3-dioxygenase subunit beta;
DE EC=1.14.12.11;
GN Name=bnzB; Synonyms=todC2;
OS Pseudomonas putida (Arthrobacter siderocapsulatus).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=303;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BE-81;
RX PubMed=3667527; DOI=10.1128/jb.169.11.5174-5179.1987;
RA Irie S., Doi S., Yorifuji T., Takagi M., Yano K.;
RT "Nucleotide sequencing and characterization of the genes encoding benzene
RT oxidation enzymes of Pseudomonas putida.";
RL J. Bacteriol. 169:5174-5179(1987).
CC -!- FUNCTION: Catalyzes both the oxidation of benzene and toluene. The beta
CC subunit may be responsible for the substrate specificity of the enzyme.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=benzene + H(+) + NADH + O2 = cis-1,2-dihydrobenzene-1,2-diol +
CC NAD(+); Xref=Rhea:RHEA:13813, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16190, ChEBI:CHEBI:16716, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.14.12.3;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + NADH + O2 + toluene = (1S,2R)-3-methylcyclohexa-3,5-
CC diene-1,2-diol + NAD(+); Xref=Rhea:RHEA:16737, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:15565, ChEBI:CHEBI:17578,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.14.12.11;
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Evidence={ECO:0000250};
CC Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000250};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC Note=Binds 1 Fe cation per subunit. {ECO:0000250};
CC -!- PATHWAY: Aromatic compound metabolism; benzene degradation; catechol
CC from benzene: step 1/2.
CC -!- PATHWAY: Xenobiotic degradation; toluene degradation.
CC -!- PATHWAY: Xenobiotic degradation; xylene degradation.
CC -!- SUBUNIT: This dioxygenase system consists of four proteins: the two
CC subunits of the hydroxylase component (BnzA and BnzB), a ferredoxin
CC (BnzC) and a ferredoxin reductase (BnzD).
CC -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC beta subunit family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M17904; AAA25736.1; -; Genomic_DNA.
DR PIR; B29830; B29830.
DR RefSeq; WP_012052600.1; NZ_NHBC01000013.1.
DR AlphaFoldDB; P0C619; -.
DR SMR; P0C619; -.
DR UniPathway; UPA00228; -.
DR UniPathway; UPA00272; UER00391.
DR UniPathway; UPA00273; -.
DR GO; GO:0018619; F:benzene 1,2-dioxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0018624; F:toluene dioxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0042203; P:toluene catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0042184; P:xylene catabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00667; ring_hydroxylating_dioxygenases_beta; 1.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR000391; Rng_hydr_dOase-bsu.
DR PANTHER; PTHR41534; PTHR41534; 1.
DR Pfam; PF00866; Ring_hydroxyl_B; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism; Dioxygenase; NAD; Oxidoreductase.
FT CHAIN 1..187
FT /note="Benzene 1,2-dioxygenase subunit beta"
FT /id="PRO_0000085067"
SQ SEQUENCE 187 AA; 22013 MW; 39A5D2BECB6116B5 CRC64;
MIDSANRADV FLRKPAPVAP ELQHEVEQFY YWEAKLLNDR RFEEWFALLA EDIHYFMPIR
TTRIMRDSRL EYSGSREYAH FDDDATMMKG RLRKITSDVS WSENPASRTR HLVSNVMIVG
AEAEGEYEIS SAFIVYRNRL ERQLDIFAGE RRDTLRRNTS EAGFEIVNRT ILIDQSTILA
NNLSFFF
