ID   BNZE_PSEPU              Reviewed;         275 AA.
AC   P08088;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 2.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Cis-1,2-dihydrobenzene-1,2-diol dehydrogenase;
DE            EC=1.3.1.19;
DE   AltName: Full=Cis-benzene glycol dehydrogenase;
GN   Name=bnzE;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BE-81;
RX   PubMed=3667527; DOI=10.1128/jb.169.11.5174-5179.1987;
RA   Irie S., Doi S., Yorifuji T., Takagi M., Yano K.;
RT   "Nucleotide sequencing and characterization of the genes encoding benzene
RT   oxidation enzymes of Pseudomonas putida.";
RL   J. Bacteriol. 169:5174-5179(1987).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cis-1,2-dihydrobenzene-1,2-diol + NAD(+) = catechol + H(+) +
CC         NADH; Xref=Rhea:RHEA:15457, ChEBI:CHEBI:15378, ChEBI:CHEBI:16190,
CC         ChEBI:CHEBI:18135, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.19;
CC   -!- PATHWAY: Aromatic compound metabolism; benzene degradation; catechol
CC       from benzene: step 2/2.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA25739.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M17904; AAA25739.1; ALT_INIT; Genomic_DNA.
DR   PIR; E36516; E36516.
DR   RefSeq; WP_012052597.1; NZ_BBNC01000005.1.
DR   AlphaFoldDB; P08088; -.
DR   SMR; P08088; -.
DR   UniPathway; UPA00272; UER00392.
DR   GO; GO:0018504; F:cis-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; IDA:CACAO.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR017711; BphB_TodD.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR03325; BphB_TodD; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT   CHAIN           1..275
FT                   /note="Cis-1,2-dihydrobenzene-1,2-diol dehydrogenase"
FT                   /id="PRO_0000054530"
FT   ACT_SITE        155
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         9..33
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         142
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   275 AA;  28782 MW;  D5919208D859234B CRC64;
     MRLEGEVALV TGGGAGLGRA IVDRYVAEGA RVAVLDKSAA GLEALRKLHG DAIVGVEGDV
     RSLDSHREAV ARCVEAFGKL DCLVGNAGVW DYLTQLVDIP DDLISEAFEE MFEVNVKGYI
     LAAKAALPAL YQSKGSAIFT VSNAGFYPGG GGVLYTAGKH AVIGLIKQLA HEWGPRIRVN
     GIAPGGILGS DLRGLKSLDL QDKSISTFPL DDMLKSVLPT GRAATAEEYA GAYVFFATRG
     DTVPLTGSVL NFDGGMGVRG LFEASLGAQL DKHFG