BOFC_BACSU
ID BOFC_BACSU Reviewed; 170 AA.
AC O05391;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Protein BofC;
DE AltName: Full=Bypass-of-forespore protein C;
DE Flags: Precursor;
GN Name=bofC; OrderedLocusNames=BSU27750;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / PY79;
RX PubMed=9025289; DOI=10.1099/00221287-143-1-157;
RA Gomez M., Cutting S.M.;
RT "BofC encodes a putative forespore regulator of the Bacillus subtilis sigma
RT K checkpoint.";
RL Microbiology 143:157-170(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Tosato V., Bolotin A., Bertani I., Valentino I., Bruschi C.V.;
RT "A 17.8 kb segment in the spoVB-nadC region of the Bacillus subtilis 168
RT chromosome: sequencing and ruv operon identification.";
RL Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP STRUCTURE BY NMR OF 31-170, AND SUBUNIT.
RX PubMed=16049010; DOI=10.1074/jbc.m506910200;
RA Patterson H.M., Brannigan J.A., Cutting S.M., Wilson K.S., Wilkinson A.J.,
RA Ab E., Diercks T., de Jong R.N., Truffault V., Folkers G.E., Kaptein R.;
RT "The structure of bypass of forespore C, an intercompartmental signaling
RT factor during sporulation in Bacillus.";
RL J. Biol. Chem. 280:36214-36220(2005).
CC -!- FUNCTION: Inhibits the SpoIVB zymogen from undergoing autocatalytic
CC activation by an unknown mechanism, and in this way plays a role in the
CC sigma-K checkpoint of sporulation.
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:16049010}.
CC -!- SUBCELLULAR LOCATION: Forespore intermembrane space.
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DR EMBL; X93081; CAA63621.1; -; Genomic_DNA.
DR EMBL; Y15896; CAB75329.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14735.1; -; Genomic_DNA.
DR PIR; A69596; A69596.
DR RefSeq; NP_390653.1; NC_000964.3.
DR RefSeq; WP_003229715.1; NZ_JNCM01000036.1.
DR PDB; 2BW2; NMR; -; A=31-170.
DR PDBsum; 2BW2; -.
DR AlphaFoldDB; O05391; -.
DR BMRB; O05391; -.
DR SMR; O05391; -.
DR STRING; 224308.BSU27750; -.
DR PaxDb; O05391; -.
DR PRIDE; O05391; -.
DR DNASU; 937526; -.
DR EnsemblBacteria; CAB14735; CAB14735; BSU_27750.
DR GeneID; 937526; -.
DR KEGG; bsu:BSU27750; -.
DR PATRIC; fig|224308.179.peg.3015; -.
DR eggNOG; ENOG5032U7R; Bacteria.
DR OMA; YFGITED; -.
DR BioCyc; BSUB:BSU27750-MON; -.
DR EvolutionaryTrace; O05391; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 3.10.20.420; -; 1.
DR Gene3D; 3.30.70.1740; -; 1.
DR InterPro; IPR015050; BofC_C.
DR InterPro; IPR038117; BofC_C_sf.
DR InterPro; IPR015071; BOFC_N.
DR InterPro; IPR038118; BOFC_N_sf.
DR Pfam; PF08955; BofC_C; 1.
DR Pfam; PF08977; BOFC_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Signal; Sporulation.
FT SIGNAL 1..30
FT /evidence="ECO:0000305"
FT CHAIN 31..170
FT /note="Protein BofC"
FT /id="PRO_0000020824"
FT VARIANT 96
FT /note="S -> F (in BofC1)"
FT STRAND 34..48
FT /evidence="ECO:0007829|PDB:2BW2"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:2BW2"
FT STRAND 54..65
FT /evidence="ECO:0007829|PDB:2BW2"
FT HELIX 66..72
FT /evidence="ECO:0007829|PDB:2BW2"
FT STRAND 76..82
FT /evidence="ECO:0007829|PDB:2BW2"
FT STRAND 85..91
FT /evidence="ECO:0007829|PDB:2BW2"
FT HELIX 98..100
FT /evidence="ECO:0007829|PDB:2BW2"
FT STRAND 105..108
FT /evidence="ECO:0007829|PDB:2BW2"
FT TURN 109..111
FT /evidence="ECO:0007829|PDB:2BW2"
FT STRAND 112..119
FT /evidence="ECO:0007829|PDB:2BW2"
FT TURN 135..137
FT /evidence="ECO:0007829|PDB:2BW2"
FT HELIX 140..148
FT /evidence="ECO:0007829|PDB:2BW2"
FT HELIX 155..168
FT /evidence="ECO:0007829|PDB:2BW2"
SQ SEQUENCE 170 AA; 19296 MW; 2786FEE1DBDFD2A2 CRC64;
MKRFSTAYLL LGILCSAAVF LIGAPSRALG AEVEHYEPLQ VHVQLEKVYL DGDVSIEHKH
EKVFSMDDFW AAYAGWTLVE QKKGYVLFRK QMDDISPLSK VNGYIGVSDN GVISTFHGRP
EPASEPIQSF FQIDLERLES HMQKNLLKGI PFRTKAEFED VIEHMKTYSG
