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SYT11_RAT
ID   SYT11_RAT               Reviewed;         430 AA.
AC   O08835; Q925B6;
DT   18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2003, sequence version 2.
DT   03-AUG-2022, entry version 158.
DE   RecName: Full=Synaptotagmin-11 {ECO:0000305};
DE   AltName: Full=Synaptotagmin XI;
DE            Short=SytXI;
GN   Name=Syt11 {ECO:0000312|RGD:62042};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, MUTAGENESIS OF SER-247, AND
RP   FUNCTION.
RC   TISSUE=Brain;
RX   PubMed=9162066; DOI=10.1074/jbc.272.22.14314;
RA   von Poser C., Ichtchenko K., Shao X., Rizo J., Suedhof T.C.;
RT   "The evolutionary pressure to inactivate. A subclass of synaptotagmins with
RT   an amino acid substitution that abolishes Ca2+ binding.";
RL   J. Biol. Chem. 272:14314-14319(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Shin O.-H., von Poser C., Ichtchenko K., Shao X., Rizo J., Suedhof T.C.;
RL   Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   INTERACTION WITH AGO2 AND SND1.
RX   PubMed=24882364; DOI=10.1016/j.febslet.2014.05.031;
RA   Milochau A., Lagree V., Benassy M.N., Chaignepain S., Papin J.,
RA   Garcia-Arcos I., Lajoix A., Monterrat C., Coudert L., Schmitter J.M.,
RA   Ochoa B., Lang J.;
RT   "Synaptotagmin 11 interacts with components of the RNA-induced silencing
RT   complex RISC in clonal pancreatic beta-cells.";
RL   FEBS Lett. 588:2217-2222(2014).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND MUTAGENESIS OF 345-LYS-LYS-346.
RX   PubMed=26589353; DOI=10.15252/embr.201540689;
RA   Wang C., Wang Y., Hu M., Chai Z., Wu Q., Huang R., Han W., Zhang C.X.,
RA   Zhou Z.;
RT   "Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis.";
RL   EMBO Rep. 17:47-63(2016).
RN   [5]
RP   UBIQUITINATED, DOMAIN, AND FUNCTION.
RX   PubMed=29311685; DOI=10.1038/s41467-017-02593-y;
RA   Wang C., Kang X., Zhou L., Chai Z., Wu Q., Huang R., Xu H., Hu M., Sun X.,
RA   Sun S., Li J., Jiao R., Zuo P., Zheng L., Yue Z., Zhou Z.;
RT   "Synaptotagmin-11 is a critical mediator of parkin-linked neurotoxicity and
RT   Parkinson's disease-like pathology.";
RL   Nat. Commun. 9:81-81(2018).
RN   [6]
RP   INTERACTION WITH KIF1A.
RX   PubMed=30021165; DOI=10.1016/j.celrep.2018.06.071;
RA   Stucchi R., Plucinska G., Hummel J.J.A., Zahavi E.E., Guerra San Juan I.,
RA   Klykov O., Scheltema R.A., Altelaar A.F.M., Hoogenraad C.C.;
RT   "Regulation of KIF1A-Driven Dense Core Vesicle Transport: Ca2+/CaM Controls
RT   DCV Binding and Liprin-alpha/TANC2 Recruits DCVs to Postsynaptic Sites.";
RL   Cell Rep. 24:685-700(2018).
CC   -!- FUNCTION: Synaptotagmin family member involved in vesicular and
CC       membrane trafficking which does not bind Ca(2+) (PubMed:9162066).
CC       Inhibits clathrin-mediated and bulk endocytosis in neurons, functions
CC       to ensure precision in vesicle retrieval (PubMed:29311685,
CC       PubMed:26589353). Plays an important role in dopamine transmission by
CC       regulating endocytosis and the vesicle-recycling process
CC       (PubMed:29311685). Essential component of a neuronal vesicular
CC       trafficking pathway that differs from the synaptic vesicle trafficking
CC       pathway but is crucial for development and synaptic plasticity. In
CC       macrophages and microglia, inhibits the conventional cytokine
CC       secretion, of at least IL6 and TNF, and phagocytosis. In astrocytes,
CC       regulates lysosome exocytosis, mechanism required for the repair of
CC       injured astrocyte cell membrane (By similarity). Required for the
CC       ATP13A2-mediated regulation of the autophagy-lysosome pathway (By
CC       similarity). {ECO:0000250|UniProtKB:Q9BT88,
CC       ECO:0000250|UniProtKB:Q9R0N3, ECO:0000269|PubMed:26589353,
CC       ECO:0000269|PubMed:29311685, ECO:0000269|PubMed:9162066}.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00041};
CC   -!- SUBUNIT: Homodimer. Can also form heterodimers. Interacts with PRKN (By
CC       similarity). Interacts (via C2 2 domain) with AGO2 and SND1; the
CC       interaction with SND1 is direct (PubMed:24882364). Interacts with
CC       KIF1A; the interaction increases in presence of calcium
CC       (PubMed:30021165). {ECO:0000250|UniProtKB:Q9BT88,
CC       ECO:0000269|PubMed:24882364, ECO:0000269|PubMed:30021165}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC       {ECO:0000250|UniProtKB:Q9R0N3}; Single-pass membrane protein
CC       {ECO:0000305}. Perikaryon {ECO:0000250|UniProtKB:Q9R0N3}. Golgi
CC       apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:Q9R0N3};
CC       Single-pass membrane protein {ECO:0000250|UniProtKB:Q9R0N3}. Recycling
CC       endosome membrane {ECO:0000269|PubMed:26589353}; Single-pass membrane
CC       protein {ECO:0000305}. Lysosome membrane
CC       {ECO:0000250|UniProtKB:Q9R0N3}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q9R0N3}. Cytoplasmic vesicle, phagosome
CC       {ECO:0000250|UniProtKB:Q9R0N3}. Cell projection, axon
CC       {ECO:0000269|PubMed:26589353}. Cell projection, dendrite
CC       {ECO:0000250|UniProtKB:Q9R0N3}. Postsynaptic density
CC       {ECO:0000250|UniProtKB:Q9R0N3}. Cytoplasmic vesicle, clathrin-coated
CC       vesicle membrane {ECO:0000269|PubMed:26589353}; Single-pass membrane
CC       protein {ECO:0000305}. Perikaryon {ECO:0000269|PubMed:26589353}.
CC       Note=Localized in vesicles that travels in axonal and dendritic shafts
CC       in both anterograde and retrograde directions. In macrophages and
CC       microglia, recruited in phagosomes at early stages of phagocytosis.
CC       {ECO:0000250|UniProtKB:Q9R0N3}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in brain and at lower levels in
CC       other tissues. {ECO:0000269|PubMed:9162066}.
CC   -!- DOMAIN: The second C2 domain/C2B is required for the inhibitory role in
CC       both clathrin-mediated and bulk endocytosis (PubMed:26589353,
CC       PubMed:29311685). The transmembrane domain and the first C2 domain/C2A
CC       are critical for the inhibitory role in clathrin-mediated endocytosis
CC       or bulk endocytosis, respectively (PubMed:26589353).
CC       {ECO:0000269|PubMed:26589353, ECO:0000269|PubMed:29311685}.
CC   -!- DOMAIN: Unlike in other synaptotagmin family members, the first C2
CC       domain/C2A does not bind Ca(2+) neither mediates Ca(2+)-dependent
CC       phospholipid binding. An aspartate-to-serine substitution in this
CC       domain inactivates Ca(2+)/phospho-lipid binding.
CC       {ECO:0000269|PubMed:9162066}.
CC   -!- PTM: Ubiquitinated, at least by PRKN, and targeted to the proteasome
CC       complex for degradation (PubMed:29311685). Ubiquitination is inhibited
CC       by ATP13A2 (By similarity). {ECO:0000250|UniProtKB:Q9BT88,
CC       ECO:0000269|PubMed:29311685}.
CC   -!- SIMILARITY: Belongs to the synaptotagmin family. {ECO:0000305}.
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DR   EMBL; AF000423; AAB58344.1; -; mRNA.
DR   EMBL; AF375465; AAK56960.1; -; mRNA.
DR   RefSeq; XP_006232810.1; XM_006232748.1.
DR   AlphaFoldDB; O08835; -.
DR   SMR; O08835; -.
DR   BioGRID; 248858; 1.
DR   STRING; 10116.ENSRNOP00000027474; -.
DR   iPTMnet; O08835; -.
DR   PhosphoSitePlus; O08835; -.
DR   PaxDb; O08835; -.
DR   PRIDE; O08835; -.
DR   GeneID; 60568; -.
DR   UCSC; RGD:62042; rat.
DR   CTD; 23208; -.
DR   RGD; 62042; Syt11.
DR   VEuPathDB; HostDB:ENSRNOG00000020279; -.
DR   eggNOG; KOG1028; Eukaryota.
DR   HOGENOM; CLU_023008_7_3_1; -.
DR   InParanoid; O08835; -.
DR   OMA; LQVINHD; -.
DR   OrthoDB; 925064at2759; -.
DR   PhylomeDB; O08835; -.
DR   Reactome; R-RNO-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR   Reactome; R-RNO-8856828; Clathrin-mediated endocytosis.
DR   PRO; PR:O08835; -.
DR   Proteomes; UP000002494; Chromosome 2.
DR   Bgee; ENSRNOG00000020279; Expressed in frontal cortex and 20 other tissues.
DR   Genevisible; O08835; RN.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0030424; C:axon; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0044297; C:cell body; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0030665; C:clathrin-coated vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0030425; C:dendrite; ISS:UniProtKB.
DR   GO; GO:0043197; C:dendritic spine; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0032009; C:early phagosome; ISS:UniProtKB.
DR   GO; GO:0060076; C:excitatory synapse; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0070382; C:exocytic vesicle; IBA:GO_Central.
DR   GO; GO:0060077; C:inhibitory synapse; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0005887; C:integral component of plasma membrane; ISO:RGD.
DR   GO; GO:0099059; C:integral component of presynaptic active zone membrane; IDA:SynGO.
DR   GO; GO:0030285; C:integral component of synaptic vesicle membrane; IDA:SynGO.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR   GO; GO:0043005; C:neuron projection; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0001891; C:phagocytic cup; ISO:RGD.
DR   GO; GO:0045335; C:phagocytic vesicle; ISO:RGD.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0098794; C:postsynapse; IDA:SynGO.
DR   GO; GO:0014069; C:postsynaptic density; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0098793; C:presynapse; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0048787; C:presynaptic active zone membrane; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0055037; C:recycling endosome; ISS:UniProtKB.
DR   GO; GO:0055038; C:recycling endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0098685; C:Schaffer collateral - CA1 synapse; IDA:SynGO.
DR   GO; GO:0045202; C:synapse; IBA:GO_Central.
DR   GO; GO:0008021; C:synaptic vesicle; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0043195; C:terminal bouton; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0005802; C:trans-Golgi network; ISS:UniProtKB.
DR   GO; GO:0031982; C:vesicle; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0048487; F:beta-tubulin binding; ISO:RGD.
DR   GO; GO:0030276; F:clathrin binding; IBA:GO_Central.
DR   GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0001786; F:phosphatidylserine binding; IBA:GO_Central.
DR   GO; GO:0000149; F:SNARE binding; ISO:RGD.
DR   GO; GO:0031369; F:translation initiation factor binding; ISO:RGD.
DR   GO; GO:0031625; F:ubiquitin protein ligase binding; ISO:RGD.
DR   GO; GO:0006914; P:autophagy; ISS:UniProtKB.
DR   GO; GO:1990927; P:calcium ion regulated lysosome exocytosis; ISO:RGD.
DR   GO; GO:0017156; P:calcium-ion regulated exocytosis; IBA:GO_Central.
DR   GO; GO:0071277; P:cellular response to calcium ion; IBA:GO_Central.
DR   GO; GO:0051650; P:establishment of vesicle localization; ISS:UniProtKB.
DR   GO; GO:0007612; P:learning; ISS:UniProtKB.
DR   GO; GO:0007613; P:memory; ISS:UniProtKB.
DR   GO; GO:1905469; P:negative regulation of clathrin-coated pit assembly; IMP:ParkinsonsUK-UCL.
DR   GO; GO:1900186; P:negative regulation of clathrin-dependent endocytosis; IMP:ParkinsonsUK-UCL.
DR   GO; GO:0001818; P:negative regulation of cytokine production; ISS:UniProtKB.
DR   GO; GO:0033602; P:negative regulation of dopamine secretion; IDA:UniProtKB.
DR   GO; GO:0045806; P:negative regulation of endocytosis; IDA:UniProtKB.
DR   GO; GO:0032715; P:negative regulation of interleukin-6 production; ISS:UniProtKB.
DR   GO; GO:1905154; P:negative regulation of membrane invagination; IMP:ParkinsonsUK-UCL.
DR   GO; GO:1903979; P:negative regulation of microglial cell activation; ISS:UniProtKB.
DR   GO; GO:0050765; P:negative regulation of phagocytosis; ISS:UniProtKB.
DR   GO; GO:1900243; P:negative regulation of synaptic vesicle endocytosis; IMP:ParkinsonsUK-UCL.
DR   GO; GO:0032720; P:negative regulation of tumor necrosis factor production; ISS:UniProtKB.
DR   GO; GO:0001778; P:plasma membrane repair; ISO:RGD.
DR   GO; GO:1905171; P:positive regulation of protein localization to phagocytic vesicle; ISO:RGD.
DR   GO; GO:0017158; P:regulation of calcium ion-dependent exocytosis; IBA:GO_Central.
DR   GO; GO:1900424; P:regulation of defense response to bacterium; ISO:RGD.
DR   GO; GO:0014059; P:regulation of dopamine secretion; IBA:GO_Central.
DR   GO; GO:0009611; P:response to wounding; ISO:RGD.
DR   GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR   GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR   Gene3D; 2.60.40.150; -; 2.
DR   InterPro; IPR000008; C2_dom.
DR   InterPro; IPR035892; C2_domain_sf.
DR   InterPro; IPR001565; Synaptotagmin.
DR   InterPro; IPR028699; SYT11.
DR   PANTHER; PTHR10024:SF115; PTHR10024:SF115; 1.
DR   Pfam; PF00168; C2; 2.
DR   PRINTS; PR00399; SYNAPTOTAGMN.
DR   SMART; SM00239; C2; 2.
DR   SUPFAM; SSF49562; SSF49562; 2.
DR   PROSITE; PS50004; C2; 2.
PE   1: Evidence at protein level;
KW   Calcium; Cell projection; Cytoplasmic vesicle; Endosome; Golgi apparatus;
KW   Lysosome; Membrane; Metal-binding; Phosphoprotein; Reference proteome;
KW   Repeat; Synapse; Transmembrane; Transmembrane helix; Ubl conjugation.
FT   CHAIN           1..430
FT                   /note="Synaptotagmin-11"
FT                   /id="PRO_0000183971"
FT   TOPO_DOM        1..15
FT                   /note="Vesicular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        37..430
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          156..278
FT                   /note="C2 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   DOMAIN          290..425
FT                   /note="C2 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   REGION          132..154
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        136..154
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         249
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   BINDING         252
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   BINDING         255
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   MOD_RES         133
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9R0N3"
FT   MUTAGEN         247
FT                   /note="S->D: Restores Ca(2+)-binding and Ca(2+)-dependent
FT                   phospholipid binding."
FT                   /evidence="ECO:0000269|PubMed:9162066"
FT   MUTAGEN         345..346
FT                   /note="KK->AA: Loss of inhibition of clathrin-mediated and
FT                   bulk endocytosis."
FT                   /evidence="ECO:0000269|PubMed:26589353"
FT   CONFLICT        74
FT                   /note="K -> E (in Ref. 1; AAB58344)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   430 AA;  48268 MW;  FEEB06702BAC2AFB CRC64;
     MAEITNIRPS FDVSPVAAGL IGASVLVVCV SVTVFVWTCC HQQAEKKHKT PPYKFIHMLK
     GISIYPETLS NKKKIIKVRR DKDGSHRESG RGNLLVNAES GLLSHDRDPR GPSPASCIDQ
     LPIKRDYGEE LRSPMTSLTP GESKPTSPSS PEEDVMLGSL TFSVDYNFPK KALVVTIQEA
     HGLPVMDGQT QGSDPYIKMT ILPDKRHRVK TRVLRKTLDP VFDETFTFYG IPYSQLQDLV
     LHFLVLSFDR FSRDDVIGEV MVPLAGVDPS TGKVQLTRDI IKRNIQKCIS RGELQVSLSY
     QPVAQRMTVV VLKARHLPKM DITGLSGNPY VKVNVYYGRK RIAKKKTHVK KCTLNPIFNE
     SFIYDIPTDL LPDISIEFLV IDFDRTTKNE VVGRLILGAH SVTTSGAEHW REVCESPRKP
     VAKWHSLSEY
 
 
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