SYT12_HUMAN
ID SYT12_HUMAN Reviewed; 421 AA.
AC Q8IV01;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Synaptotagmin-12 {ECO:0000250|UniProtKB:Q920N7};
DE AltName: Full=Synaptotagmin XII {ECO:0000250|UniProtKB:Q920N7};
DE Short=SytXII {ECO:0000250|UniProtKB:Q920N7};
GN Name=SYT12;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: Synaptic vesicle phosphoprotein that enhances spontaneous
CC neurotransmitter release but does not effect induced neurotransmitter
CC release (By similarity). Unlike other synaptotagmins, it does not bind
CC Ca(2+) or phospholipids (By similarity). Essential for mossy-fiber
CC long-term potentiation in the hippocampus (By similarity).
CC {ECO:0000250|UniProtKB:P97610, ECO:0000250|UniProtKB:Q920N7}.
CC -!- SUBUNIT: Homodimer (By similarity). Can also form heterodimers (By
CC similarity). Interacts with SYT1 (By similarity).
CC {ECO:0000250|UniProtKB:P97610, ECO:0000250|UniProtKB:Q9R0N7}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic
CC vesicle membrane {ECO:0000250|UniProtKB:P97610}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- PTM: Phosphorylation of Ser-97 is required for mossy-fiber long-term
CC potentiation. {ECO:0000250|UniProtKB:Q920N7}.
CC -!- SIMILARITY: Belongs to the synaptotagmin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BC037406; AAH37406.1; -; mRNA.
DR CCDS; CCDS8154.1; -.
DR RefSeq; NP_001171351.1; NM_001177880.1.
DR RefSeq; NP_808878.1; NM_177963.3.
DR RefSeq; XP_011543649.1; XM_011545347.1.
DR AlphaFoldDB; Q8IV01; -.
DR SMR; Q8IV01; -.
DR BioGRID; 124864; 23.
DR IntAct; Q8IV01; 14.
DR STRING; 9606.ENSP00000377520; -.
DR iPTMnet; Q8IV01; -.
DR PhosphoSitePlus; Q8IV01; -.
DR BioMuta; SYT12; -.
DR DMDM; 33151152; -.
DR MassIVE; Q8IV01; -.
DR PaxDb; Q8IV01; -.
DR PeptideAtlas; Q8IV01; -.
DR PRIDE; Q8IV01; -.
DR ProteomicsDB; 70636; -.
DR Antibodypedia; 2614; 195 antibodies from 30 providers.
DR DNASU; 91683; -.
DR Ensembl; ENST00000393946.6; ENSP00000377520.2; ENSG00000173227.14.
DR Ensembl; ENST00000525457.5; ENSP00000431400.1; ENSG00000173227.14.
DR Ensembl; ENST00000527043.6; ENSP00000435316.1; ENSG00000173227.14.
DR GeneID; 91683; -.
DR KEGG; hsa:91683; -.
DR MANE-Select; ENST00000527043.6; ENSP00000435316.1; NM_177963.4; NP_808878.1.
DR UCSC; uc001oju.4; human.
DR CTD; 91683; -.
DR DisGeNET; 91683; -.
DR GeneCards; SYT12; -.
DR HGNC; HGNC:18381; SYT12.
DR HPA; ENSG00000173227; Group enriched (brain, liver, parathyroid gland).
DR MIM; 606436; gene.
DR neXtProt; NX_Q8IV01; -.
DR OpenTargets; ENSG00000173227; -.
DR PharmGKB; PA38321; -.
DR VEuPathDB; HostDB:ENSG00000173227; -.
DR eggNOG; KOG1028; Eukaryota.
DR GeneTree; ENSGT00940000158627; -.
DR HOGENOM; CLU_053862_0_0_1; -.
DR InParanoid; Q8IV01; -.
DR OMA; LQPFGGW; -.
DR OrthoDB; 925064at2759; -.
DR PhylomeDB; Q8IV01; -.
DR TreeFam; TF315600; -.
DR PathwayCommons; Q8IV01; -.
DR Reactome; R-HSA-6794361; Neurexins and neuroligins.
DR SignaLink; Q8IV01; -.
DR BioGRID-ORCS; 91683; 23 hits in 1069 CRISPR screens.
DR ChiTaRS; SYT12; human.
DR GenomeRNAi; 91683; -.
DR Pharos; Q8IV01; Tbio.
DR PRO; PR:Q8IV01; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q8IV01; protein.
DR Bgee; ENSG00000173227; Expressed in right hemisphere of cerebellum and 138 other tissues.
DR ExpressionAtlas; Q8IV01; baseline and differential.
DR Genevisible; Q8IV01; HS.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0070382; C:exocytic vesicle; IBA:GO_Central.
DR GO; GO:0098686; C:hippocampal mossy fiber to CA3 synapse; IEA:Ensembl.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0030672; C:synaptic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR GO; GO:0005544; F:calcium-dependent phospholipid binding; IBA:GO_Central.
DR GO; GO:0030276; F:clathrin binding; IBA:GO_Central.
DR GO; GO:0001786; F:phosphatidylserine binding; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0019905; F:syntaxin binding; IBA:GO_Central.
DR GO; GO:0017156; P:calcium-ion regulated exocytosis; IBA:GO_Central.
DR GO; GO:0071277; P:cellular response to calcium ion; IBA:GO_Central.
DR GO; GO:0060291; P:long-term synaptic potentiation; IEA:Ensembl.
DR GO; GO:0017158; P:regulation of calcium ion-dependent exocytosis; IBA:GO_Central.
DR GO; GO:0014059; P:regulation of dopamine secretion; IBA:GO_Central.
DR GO; GO:0046928; P:regulation of neurotransmitter secretion; IEA:InterPro.
DR GO; GO:0048792; P:spontaneous exocytosis of neurotransmitter; IEA:Ensembl.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 2.60.40.150; -; 2.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR030537; SYT12.
DR PANTHER; PTHR10024:SF252; PTHR10024:SF252; 1.
DR Pfam; PF00168; C2; 2.
DR SMART; SM00239; C2; 2.
DR SUPFAM; SSF49562; SSF49562; 2.
DR PROSITE; PS50004; C2; 2.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Membrane; Phosphoprotein; Reference proteome; Repeat;
KW Synapse; Transmembrane; Transmembrane helix.
FT CHAIN 1..421
FT /note="Synaptotagmin-12"
FT /id="PRO_0000183972"
FT TOPO_DOM 1..18
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 40..421
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 152..272
FT /note="C2 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 283..416
FT /note="C2 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT MOD_RES 97
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P97610"
FT MOD_RES 99
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P97610"
FT MOD_RES 214
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q920N7"
FT VARIANT 170
FT /note="N -> H (in dbSNP:rs11227664)"
FT /id="VAR_034532"
SQ SEQUENCE 421 AA; 46537 MW; 9E1B3ACE0023F973 CRC64;
MAVDVAEYHL SVIKSPPGWE VGVYAAGALA LLGIAAVSLW KLWTSGSFPS PSPFPNYDYR
YLQQKYGESC AEAREKRVPA WNAQRASTRG PPSRKGSLSI EDTFESISEL GPLELMGREL
DLAPYGTLRK SQSADSLNSI SSVSNTFGQD FTLGQVEVSM EYDTASHTLN VAVMQGKDLL
EREEASFESC FMRVSLLPDE QIVGISRIQR NAYSIFFDEK FSIPLDPTAL EEKSLRFSVF
GIDEDERNVS TGVVELKLSV LDLPLQPFSG WLYLQDQNKA ADAVGEILLS LSYLPTAERL
TVVVVKAKNL IWTNDKTTAD PFVKVYLLQD GRKMSKKKTA VKRDDPNPVF NEAMIFSVPA
IVLQDLSLRV TVAESSSDGR GDNVGHVIIG PSASGMGTTH WNQMLATLRR PVSMWHAVRR
N
