SYT12_RAT
ID SYT12_RAT Reviewed; 421 AA.
AC P97610;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Synaptotagmin-12 {ECO:0000303|PubMed:17190793};
DE AltName: Full=Synaptotagmin XII {ECO:0000250|UniProtKB:Q920N7};
DE Short=SytXII {ECO:0000250|UniProtKB:Q920N7};
DE AltName: Full=Synaptotagmin-related gene 1 protein {ECO:0000303|PubMed:8987811};
DE Short=Srg1 {ECO:0000303|PubMed:8987811};
GN Name=Syt12 {ECO:0000303|PubMed:17190793};
GN Synonyms=Srg1 {ECO:0000303|PubMed:8987811}, Sytr1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley;
RX PubMed=8987811; DOI=10.1523/jneurosci.16-24-07832.1996;
RA Thompson C.C.;
RT "Thyroid hormone-responsive genes in developing cerebellum include a novel
RT synaptotagmin and a hairless homolog.";
RL J. Neurosci. 16:7832-7840(1996).
RN [2]
RP FUNCTION, INTERACTION WITH SYT1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP PHOSPHORYLATION AT SER-97, AND MUTAGENESIS OF THR-87 AND SER-97.
RX PubMed=17190793; DOI=10.1083/jcb.200607021;
RA Maximov A., Shin O.H., Liu X., Suedhof T.C.;
RT "Synaptotagmin-12, a synaptic vesicle phosphoprotein that modulates
RT spontaneous neurotransmitter release.";
RL J. Cell Biol. 176:113-124(2007).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97; SER-99 AND SER-214, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Synaptic vesicle phosphoprotein that enhances spontaneous
CC neurotransmitter release but does not effect induced neurotransmitter
CC release (PubMed:17190793). Unlike other synaptotagmins, it does not
CC bind Ca(2+) or phospholipids (PubMed:17190793). Essential for mossy-
CC fiber long-term potentiation in the hippocampus (By similarity).
CC {ECO:0000250|UniProtKB:Q920N7, ECO:0000269|PubMed:17190793}.
CC -!- SUBUNIT: Homodimer (By similarity). Can also form heterodimers (By
CC similarity). Interacts with SYT1 (PubMed:17190793).
CC {ECO:0000250|UniProtKB:Q9R0N7, ECO:0000269|PubMed:17190793}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic
CC vesicle membrane {ECO:0000269|PubMed:17190793}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in the brain, specifically in neurons of
CC the cerebellum, cortex, hippocampus, olfactory bulb, brainstem and
CC spinal cord (at protein level). {ECO:0000269|PubMed:17190793}.
CC -!- PTM: Phosphorylation of Ser-97 is required for mossy-fiber long-term
CC potentiation. {ECO:0000250|UniProtKB:Q920N7}.
CC -!- SIMILARITY: Belongs to the synaptotagmin family. {ECO:0000305}.
CC -!- CAUTION: Unlike classical synaptotagmins, lacks Ca(2+)-binding
CC sequences and does not bind phospholipids.
CC {ECO:0000269|PubMed:17190793}.
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DR EMBL; U71294; AAC53019.1; -; mRNA.
DR RefSeq; NP_620190.1; NM_138835.1.
DR AlphaFoldDB; P97610; -.
DR SMR; P97610; -.
DR BioGRID; 251324; 1.
DR IntAct; P97610; 1.
DR MINT; P97610; -.
DR STRING; 10116.ENSRNOP00000026153; -.
DR iPTMnet; P97610; -.
DR PhosphoSitePlus; P97610; -.
DR PaxDb; P97610; -.
DR PRIDE; P97610; -.
DR ABCD; P97610; 1 sequenced antibody.
DR Ensembl; ENSRNOT00000026152; ENSRNOP00000026153; ENSRNOG00000019306.
DR GeneID; 191595; -.
DR KEGG; rno:191595; -.
DR UCSC; RGD:621876; rat.
DR CTD; 91683; -.
DR RGD; 621876; Syt12.
DR eggNOG; KOG1028; Eukaryota.
DR GeneTree; ENSGT00940000158627; -.
DR HOGENOM; CLU_053862_0_0_1; -.
DR InParanoid; P97610; -.
DR OMA; LQPFGGW; -.
DR OrthoDB; 925064at2759; -.
DR PhylomeDB; P97610; -.
DR TreeFam; TF315600; -.
DR PRO; PR:P97610; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000019306; Expressed in cerebellum and 11 other tissues.
DR Genevisible; P97610; RN.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0070382; C:exocytic vesicle; IBA:GO_Central.
DR GO; GO:0098686; C:hippocampal mossy fiber to CA3 synapse; IEA:Ensembl.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0098793; C:presynapse; IDA:UniProtKB.
DR GO; GO:0030672; C:synaptic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR GO; GO:0005544; F:calcium-dependent phospholipid binding; IBA:GO_Central.
DR GO; GO:0030276; F:clathrin binding; IBA:GO_Central.
DR GO; GO:0001786; F:phosphatidylserine binding; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0019905; F:syntaxin binding; IBA:GO_Central.
DR GO; GO:0017156; P:calcium-ion regulated exocytosis; IBA:GO_Central.
DR GO; GO:0071277; P:cellular response to calcium ion; IBA:GO_Central.
DR GO; GO:0060291; P:long-term synaptic potentiation; ISO:RGD.
DR GO; GO:0017158; P:regulation of calcium ion-dependent exocytosis; IBA:GO_Central.
DR GO; GO:0014059; P:regulation of dopamine secretion; IBA:GO_Central.
DR GO; GO:0046928; P:regulation of neurotransmitter secretion; NAS:RGD.
DR GO; GO:0048792; P:spontaneous exocytosis of neurotransmitter; IDA:GO_Central.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 2.60.40.150; -; 2.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR030537; SYT12.
DR PANTHER; PTHR10024:SF252; PTHR10024:SF252; 1.
DR Pfam; PF00168; C2; 2.
DR SMART; SM00239; C2; 2.
DR SUPFAM; SSF49562; SSF49562; 2.
DR PROSITE; PS50004; C2; 2.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Membrane; Phosphoprotein; Reference proteome; Repeat;
KW Synapse; Transmembrane; Transmembrane helix.
FT CHAIN 1..421
FT /note="Synaptotagmin-12"
FT /id="PRO_0000183974"
FT TOPO_DOM 1..18
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 40..421
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 152..272
FT /note="C2 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 283..416
FT /note="C2 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT MOD_RES 97
FT /note="Phosphoserine; by PKA"
FT /evidence="ECO:0000269|PubMed:17190793,
FT ECO:0007744|PubMed:22673903"
FT MOD_RES 99
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 214
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MUTAGEN 87
FT /note="T->A: No effect on phosphorylation by PKA."
FT /evidence="ECO:0000269|PubMed:17190793"
FT MUTAGEN 97
FT /note="S->A: Loss of phosphorylation by PKA. No effect on
FT localization to synapse. Inhibits the spontaneous release
FT of neurotransmitters."
FT /evidence="ECO:0000269|PubMed:17190793"
SQ SEQUENCE 421 AA; 46608 MW; C83F745FDD050897 CRC64;
MAVDVTEYHL SVIKSPPGWE VGVYAAGALA LLGIAAVSLW KLWTSGSFPS PSPFPNYDYR
YLQQKYGEAY VEAKLKRVPP WNAQRTTTRG PPSRKGSLSI EDTFESISEL GPLELMGREL
DLAPYGTLRK SQSADSLNSI SSVSNTFGQD FTLGQVEVSM DYDGASHTLH VAVLQGKDLL
EREEATFESC FMRVSLLPDE QIVGISRIQR NAYSIFFDEK FSVPLDPTAL EEKSLRFSVF
GIDEDERNVS TGVVELKLSV LDLPLQPFSG WLYLQDQNKA ADAVGEILLS LSYLPTAERL
TVVVVKAKNL IWTNDKTTAD PFVKVYLLQD GRKMSKKKTA VKRDDPNPVF NEAMIFSVPA
IVLQDLSLRV TVAESSSDGR GDNVGHVIIG PGASGMGTTH WNQMLATLRR PVSMWHPVRR
N
