SYT2_ARATH
ID SYT2_ARATH Reviewed; 537 AA.
AC B6ETT4; Q9LNT5;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Synaptotagmin-2;
DE AltName: Full=NTMC2T1.2;
DE AltName: Full=Synaptotagmin B;
GN Name=SYT2; Synonyms=SYTB; OrderedLocusNames=At1g20080; ORFNames=T20H2.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=17672888; DOI=10.1186/1471-2164-8-259;
RA Craxton M.;
RT "Evolutionary genomics of plant genes encoding N-terminal-TM-C2 domain
RT proteins and the similar FAM62 genes and synaptotagmin genes of
RT metazoans.";
RL BMC Genomics 8:259-259(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=22140429; DOI=10.1371/journal.pone.0026477;
RA Zhang H., Zhang L., Gao B., Fan H., Jin J., Botella M.A., Jiang L., Lin J.;
RT "Golgi apparatus-localized synaptotagmin 2 is required for unconventional
RT secretion in Arabidopsis.";
RL PLoS ONE 6:E26477-E26477(2011).
CC -!- FUNCTION: May play an important role in regulating an unconventional
CC protein trafficking from the cytosol to the extracellular matrix.
CC {ECO:0000269|PubMed:22140429}.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000305|PubMed:22140429}; Single-pass membrane protein
CC {ECO:0000305|PubMed:22140429}.
CC -!- SIMILARITY: Belongs to the synaptotagmin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79904.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; FM213366; CAR82571.1; -; mRNA.
DR EMBL; AC022472; AAF79904.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29933.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60121.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60122.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60123.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60124.1; -; Genomic_DNA.
DR PIR; E86334; E86334.
DR RefSeq; NP_001319049.1; NM_001332435.1.
DR RefSeq; NP_001322429.1; NM_001332438.1.
DR RefSeq; NP_001322430.1; NM_001332436.1.
DR RefSeq; NP_001322431.1; NM_001332437.1.
DR RefSeq; NP_173436.4; NM_101862.5.
DR AlphaFoldDB; B6ETT4; -.
DR SMR; B6ETT4; -.
DR STRING; 3702.AT1G20080.1; -.
DR PaxDb; B6ETT4; -.
DR PRIDE; B6ETT4; -.
DR ProteomicsDB; 233028; -.
DR EnsemblPlants; AT1G20080.1; AT1G20080.1; AT1G20080.
DR EnsemblPlants; AT1G20080.2; AT1G20080.2; AT1G20080.
DR EnsemblPlants; AT1G20080.3; AT1G20080.3; AT1G20080.
DR EnsemblPlants; AT1G20080.4; AT1G20080.4; AT1G20080.
DR EnsemblPlants; AT1G20080.5; AT1G20080.5; AT1G20080.
DR GeneID; 838597; -.
DR Gramene; AT1G20080.1; AT1G20080.1; AT1G20080.
DR Gramene; AT1G20080.2; AT1G20080.2; AT1G20080.
DR Gramene; AT1G20080.3; AT1G20080.3; AT1G20080.
DR Gramene; AT1G20080.4; AT1G20080.4; AT1G20080.
DR Gramene; AT1G20080.5; AT1G20080.5; AT1G20080.
DR KEGG; ath:AT1G20080; -.
DR Araport; AT1G20080; -.
DR TAIR; locus:2198581; AT1G20080.
DR eggNOG; KOG1012; Eukaryota.
DR HOGENOM; CLU_028927_1_1_1; -.
DR InParanoid; B6ETT4; -.
DR OMA; VSEKSHG; -.
DR OrthoDB; 52746at2759; -.
DR PhylomeDB; B6ETT4; -.
DR PRO; PR:B6ETT4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; B6ETT4; baseline and differential.
DR Genevisible; B6ETT4; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR GO; GO:0009306; P:protein secretion; IMP:TAIR.
DR Gene3D; 2.60.40.150; -; 2.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR031468; SMP_LBD.
DR InterPro; IPR045050; Synaptotagmin_plant.
DR InterPro; IPR039010; Synaptotagmin_SMP.
DR PANTHER; PTHR10774; PTHR10774; 1.
DR Pfam; PF00168; C2; 2.
DR Pfam; PF17047; SMP_LBD; 1.
DR PRINTS; PR00360; C2DOMAIN.
DR SMART; SM00239; C2; 2.
DR SUPFAM; SSF49562; SSF49562; 2.
DR PROSITE; PS50004; C2; 2.
DR PROSITE; PS51847; SMP; 1.
PE 2: Evidence at transcript level;
KW Calcium; Golgi apparatus; Lipid transport; Lipid-binding; Membrane;
KW Metal-binding; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..537
FT /note="Synaptotagmin-2"
FT /id="PRO_0000419239"
FT TOPO_DOM 1..2
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 24..537
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT DOMAIN 67..249
FT /note="SMP-LTD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01194"
FT DOMAIN 240..362
FT /note="C2 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 402..517
FT /note="C2 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT REGION 227..505
FT /note="Phospholipid binding"
FT /evidence="ECO:0000250"
FT BINDING 276
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 282
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 332
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 334
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
SQ SEQUENCE 537 AA; 60837 MW; 9B95D589C573A878 CRC64;
MGIISTILGV IGFGFGTTIG IVIGYYLFIY FQSTDVEDPE IKPLVELDSE TIATMFPEIP
MWVKNPDFDR IDWLNKLIGH MWPYMDKAIC KMAKSIAKPI IAEQIPNYKI DSVEFEMLTL
GSLPPSFQGM KVYATDDKEI IMELSVKWAG NPNIIVVAKA FGLKATVQVI DLQVYATPRI
TLKPLVPSFP CFANIFVSLM DKPQVDFGLK LLGADVMAIP GLYRFVQEII KDQVANMYLW
PKTLNVQIMD PSKAMKKPVG LLSVKVIKAI KLKKKDLLGG SDPYVKLTLS GDKVPGKKTV
VKHSNLNPEW NEEFDLVVKE PESQELQLIV YDWEQVGKHD KIGMNVIQLK DLTPEEPKLM
TLELLKSMEP KEPVSEKSRG QLVVEVEYKP FKDDDIPENI DDPNAVEKAP EGTPSTGGLL
VVIVHEAEDL EGKYHTNPSV RLLFRGEERK TKRVKKNREP RWDEDFQFPL DEPPINDKLH
VEVISSSSRL IHPKETLGYV VINLGDVVSN RRINDKYHLI DSKNGRIQIE LQWRNSS
