SYT3_ARATH
ID SYT3_ARATH Reviewed; 540 AA.
AC Q7XA06; B6ETT6; Q9FYD9;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Synaptotagmin-3;
DE AltName: Full=NTMC2T1.3;
DE AltName: Full=Synaptotagmin C;
GN Name=SYT3; Synonyms=SYTC; OrderedLocusNames=At5g04220; ORFNames=F21E1.140;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=12801916; DOI=10.1093/jb/mvg082;
RA Fukuda M.;
RT "Molecular cloning, expression, and characterization of a novel class of
RT synaptotagmin (Syt XIV) conserved from Drosophila to humans.";
RL J. Biochem. 133:641-649(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RX PubMed=17672888; DOI=10.1186/1471-2164-8-259;
RA Craxton M.;
RT "Evolutionary genomics of plant genes encoding N-terminal-TM-C2 domain
RT proteins and the similar FAM62 genes and synaptotagmin genes of
RT metazoans.";
RL BMC Genomics 8:259-259(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: May be involved in membrane trafficking. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00041};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Single-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7XA06-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7XA06-2; Sequence=VSP_044138;
CC -!- SIMILARITY: Belongs to the synaptotagmin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAC05504.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB102952; BAC76813.1; -; mRNA.
DR EMBL; FM213367; CAR82572.1; -; mRNA.
DR EMBL; FM213368; CAR82573.1; -; mRNA.
DR EMBL; AL391716; CAC05504.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90712.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90713.1; -; Genomic_DNA.
DR RefSeq; NP_568135.1; NM_120504.2. [Q7XA06-2]
DR RefSeq; NP_974729.1; NM_203000.2. [Q7XA06-1]
DR AlphaFoldDB; Q7XA06; -.
DR SMR; Q7XA06; -.
DR STRING; 3702.AT5G04220.2; -.
DR iPTMnet; Q7XA06; -.
DR PaxDb; Q7XA06; -.
DR PRIDE; Q7XA06; -.
DR ProteomicsDB; 234109; -. [Q7XA06-1]
DR EnsemblPlants; AT5G04220.1; AT5G04220.1; AT5G04220. [Q7XA06-2]
DR EnsemblPlants; AT5G04220.2; AT5G04220.2; AT5G04220. [Q7XA06-1]
DR GeneID; 830301; -.
DR Gramene; AT5G04220.1; AT5G04220.1; AT5G04220. [Q7XA06-2]
DR Gramene; AT5G04220.2; AT5G04220.2; AT5G04220. [Q7XA06-1]
DR KEGG; ath:AT5G04220; -.
DR Araport; AT5G04220; -.
DR TAIR; locus:2146688; AT5G04220.
DR eggNOG; KOG1012; Eukaryota.
DR HOGENOM; CLU_028927_1_0_1; -.
DR InParanoid; Q7XA06; -.
DR PhylomeDB; Q7XA06; -.
DR PRO; PR:Q7XA06; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q7XA06; baseline and differential.
DR Genevisible; Q7XA06; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.150; -; 2.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR031468; SMP_LBD.
DR InterPro; IPR045050; Synaptotagmin_plant.
DR InterPro; IPR039010; Synaptotagmin_SMP.
DR PANTHER; PTHR10774; PTHR10774; 1.
DR Pfam; PF00168; C2; 2.
DR Pfam; PF17047; SMP_LBD; 1.
DR PRINTS; PR00360; C2DOMAIN.
DR SMART; SM00239; C2; 2.
DR SUPFAM; SSF49562; SSF49562; 2.
DR PROSITE; PS50004; C2; 2.
DR PROSITE; PS51847; SMP; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calcium; Lipid transport; Lipid-binding; Membrane;
KW Metal-binding; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..540
FT /note="Synaptotagmin-3"
FT /id="PRO_0000419240"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 67..249
FT /note="SMP-LTD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01194"
FT DOMAIN 240..363
FT /note="C2 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 401..521
FT /note="C2 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT REGION 227..509
FT /note="Phospholipid binding"
FT /evidence="ECO:0000250"
FT BINDING 277
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 277
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 283
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 333
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 333
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 335
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 335
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 341
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT VAR_SEQ 1..227
FT /note="MGFFTSVLGIIGFVIGIPIGLILGFFVLIYSQPSHQEYPPARPLVETSISVL
FT LDLLPDIPLWMKNPDYERVDWFNKFISYMWPYLDKAVCGIIRSSVQPLFADYIGTFCIE
FT SIEFENLSLGTLPPTVHGVKFYETNEKELLFEPSIKWAGNPNIVLVLKVLSLRIRVQLV
FT DLQFFAIVRVALKPLLPTFPCFGMVVVSLMEKPHVDFGLKVLGGDLMSIPGLYRYVQ
FT -> MILLS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17672888"
FT /id="VSP_044138"
SQ SEQUENCE 540 AA; 61870 MW; FC5FE33A40A6DB1E CRC64;
MGFFTSVLGI IGFVIGIPIG LILGFFVLIY SQPSHQEYPP ARPLVETSIS VLLDLLPDIP
LWMKNPDYER VDWFNKFISY MWPYLDKAVC GIIRSSVQPL FADYIGTFCI ESIEFENLSL
GTLPPTVHGV KFYETNEKEL LFEPSIKWAG NPNIVLVLKV LSLRIRVQLV DLQFFAIVRV
ALKPLLPTFP CFGMVVVSLM EKPHVDFGLK VLGGDLMSIP GLYRYVQETI KRQVSSMYHW
PQVLEIPILD SSTASVKKPV GLLHVSILRA RNLLKKDLLG TSDPYVKLSL TGEKLPAKKT
TIKKRNLNPE WNEHFKLIVK DPNSQVLQLE VFDWDKVGGH DRLGMQMIPL QKINPGERKE
FNLDLIKNSN VVMDSGDKKK RGRLEVDLRY VPFREESIKR RKESREEKSS EDDDFLSQAG
LLSVAVQSAK DVEGKKKHSN PYAVVLFRGE KKKTKMLKKT RDPRWNEEFQ FTLEEPPVKE
SIRVEVMSKG TGFHFRSKEE LGHVDINLDD VVDNGRINQK YHLINSRNGI IHIEIRWTTS
