BOLA2_MOUSE
ID BOLA2_MOUSE Reviewed; 86 AA.
AC Q8BGS2; Q0P601;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=BolA-like protein 2;
GN Name=Bola2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=FVB/N; TISSUE=Colon, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Liver, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP STRUCTURE BY NMR.
RX PubMed=14718656; DOI=10.1110/ps.03401004;
RA Kasai T., Inoue M., Koshiba S., Yabuki T., Aoki M., Nunokawa E., Seki E.,
RA Matsuda T., Matsuda N., Tomo Y., Shirouzu M., Terada T., Obayashi N.,
RA Hamana H., Shinya N., Tatsuguchi A., Yasuda S., Yoshida M., Hirota H.,
RA Matsuo Y., Tani K., Suzuki H., Arakawa T., Carninci P., Kawai J.,
RA Hayashizaki Y., Kigawa T., Yokoyama S.;
RT "Solution structure of a BolA-like protein from Mus musculus.";
RL Protein Sci. 13:545-548(2004).
CC -!- FUNCTION: Acts as a cytosolic iron-sulfur (Fe-S) cluster assembly
CC factor that facilitates [2Fe-2S] cluster insertion into a subset of
CC cytosolic proteins. Acts together with the monothiol glutaredoxin
CC GLRX3. {ECO:0000250|UniProtKB:Q9H3K6}.
CC -!- SUBUNIT: Interacts with GLRX3; forms a heterotrimeric complex composed
CC by two BOLA2 molecules and one GLRX3 molecule; linked by [2Fe-2S]
CC clusters. {ECO:0000250|UniProtKB:Q9H3K6}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9H3K6}. Nucleus
CC {ECO:0000250|UniProtKB:Q9H3K6}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BGS2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BGS2-2; Sequence=VSP_010094;
CC -!- SIMILARITY: Belongs to the BolA/IbaG family. {ECO:0000305}.
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DR EMBL; AK002662; BAC25001.1; -; mRNA.
DR EMBL; AK003922; BAC25060.1; -; mRNA.
DR EMBL; CH466531; EDL17416.1; -; Genomic_DNA.
DR EMBL; BC045523; AAH45523.1; -; mRNA.
DR EMBL; BC061197; AAH61197.1; -; mRNA.
DR CCDS; CCDS40129.1; -. [Q8BGS2-1]
DR RefSeq; NP_780312.1; NM_175103.3. [Q8BGS2-1]
DR PDB; 1V9J; NMR; -; A=1-86.
DR PDBsum; 1V9J; -.
DR AlphaFoldDB; Q8BGS2; -.
DR SMR; Q8BGS2; -.
DR BioGRID; 211260; 6.
DR STRING; 10090.ENSMUSP00000114873; -.
DR PhosphoSitePlus; Q8BGS2; -.
DR EPD; Q8BGS2; -.
DR MaxQB; Q8BGS2; -.
DR PaxDb; Q8BGS2; -.
DR PeptideAtlas; Q8BGS2; -.
DR PRIDE; Q8BGS2; -.
DR ProteomicsDB; 273757; -. [Q8BGS2-1]
DR ProteomicsDB; 273758; -. [Q8BGS2-2]
DR Ensembl; ENSMUST00000106369; ENSMUSP00000101977; ENSMUSG00000047721. [Q8BGS2-2]
DR Ensembl; ENSMUST00000130498; ENSMUSP00000114873; ENSMUSG00000047721. [Q8BGS2-1]
DR GeneID; 66162; -.
DR KEGG; mmu:66162; -.
DR UCSC; uc009jsj.1; mouse. [Q8BGS2-1]
DR CTD; 552900; -.
DR MGI; MGI:1913412; Bola2.
DR VEuPathDB; HostDB:ENSMUSG00000047721; -.
DR eggNOG; KOG3348; Eukaryota.
DR GeneTree; ENSGT00510000047760; -.
DR HOGENOM; CLU_109462_4_0_1; -.
DR InParanoid; Q8BGS2; -.
DR OMA; VHAFSQK; -.
DR OrthoDB; 1571278at2759; -.
DR PhylomeDB; Q8BGS2; -.
DR TreeFam; TF313141; -.
DR BioGRID-ORCS; 66162; 1 hit in 74 CRISPR screens.
DR ChiTaRS; Bola2; mouse.
DR EvolutionaryTrace; Q8BGS2; -.
DR PRO; PR:Q8BGS2; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q8BGS2; protein.
DR Bgee; ENSMUSG00000047721; Expressed in embryonic brain and 261 other tissues.
DR Genevisible; Q8BGS2; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:1990229; C:iron-sulfur cluster assembly complex; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0044571; P:[2Fe-2S] cluster assembly; ISS:UniProtKB.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IBA:GO_Central.
DR GO; GO:0097428; P:protein maturation by iron-sulfur cluster transfer; ISS:UniProtKB.
DR InterPro; IPR045115; BOL2.
DR InterPro; IPR002634; BolA.
DR InterPro; IPR036065; BolA-like_sf.
DR PANTHER; PTHR12735; PTHR12735; 1.
DR Pfam; PF01722; BolA; 1.
DR PIRSF; PIRSF003113; BolA; 1.
DR SUPFAM; SSF82657; SSF82657; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; Cytoplasm; Nucleus;
KW Reference proteome.
FT CHAIN 1..86
FT /note="BolA-like protein 2"
FT /id="PRO_0000201237"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9H3K6"
FT VAR_SEQ 55..86
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010094"
FT STRAND 1..3
FT /evidence="ECO:0007829|PDB:1V9J"
FT HELIX 5..16
FT /evidence="ECO:0007829|PDB:1V9J"
FT STRAND 19..25
FT /evidence="ECO:0007829|PDB:1V9J"
FT STRAND 28..31
FT /evidence="ECO:0007829|PDB:1V9J"
FT STRAND 35..40
FT /evidence="ECO:0007829|PDB:1V9J"
FT HELIX 43..46
FT /evidence="ECO:0007829|PDB:1V9J"
FT HELIX 49..59
FT /evidence="ECO:0007829|PDB:1V9J"
FT TURN 60..63
FT /evidence="ECO:0007829|PDB:1V9J"
FT HELIX 64..66
FT /evidence="ECO:0007829|PDB:1V9J"
FT STRAND 68..75
FT /evidence="ECO:0007829|PDB:1V9J"
FT HELIX 77..82
FT /evidence="ECO:0007829|PDB:1V9J"
SQ SEQUENCE 86 AA; 10215 MW; 76E2ADCAF3B6FAD2 CRC64;
MELSADYLRE KLRQDLEAEH VEVEDTTLNR CATSFRVLVV SAKFEGKPLL QRHRLVNECL
AEELPHIHAF EQKTLTPEQW TRQRRE
