ID   SYTC2_DICDI             Reviewed;         698 AA.
AC   Q8MP20; Q555M0; Q8T175;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Probable threonine--tRNA ligase 2, cytoplasmic;
DE            EC=6.1.1.3;
DE   AltName: Full=Threonyl-tRNA synthetase;
DE            Short=ThrRS;
GN   Name=thrS2; ORFNames=DDB_G0274545;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-
CC         threonyl-tRNA(Thr); Xref=Rhea:RHEA:24624, Rhea:RHEA-COMP:9670,
CC         Rhea:RHEA-COMP:9704, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57926, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78534, ChEBI:CHEBI:456215; EC=6.1.1.3;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; AC123513; AAM44378.1; -; Genomic_DNA.
DR   EMBL; AAFI02000012; EAL70165.1; -; Genomic_DNA.
DR   RefSeq; XP_644046.1; XM_638954.1.
DR   AlphaFoldDB; Q8MP20; -.
DR   SMR; Q8MP20; -.
DR   STRING; 44689.DDB0231249; -.
DR   PaxDb; Q8MP20; -.
DR   PRIDE; Q8MP20; -.
DR   EnsemblProtists; EAL70165; EAL70165; DDB_G0274545.
DR   GeneID; 8619476; -.
DR   KEGG; ddi:DDB_G0274545; -.
DR   dictyBase; DDB_G0274545; thrS2.
DR   eggNOG; KOG1637; Eukaryota.
DR   HOGENOM; CLU_008554_0_1_1; -.
DR   InParanoid; Q8MP20; -.
DR   OMA; HRNETHG; -.
DR   PhylomeDB; Q8MP20; -.
DR   PRO; PR:Q8MP20; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004829; F:threonine-tRNA ligase activity; ISS:dictyBase.
DR   GO; GO:0006435; P:threonyl-tRNA aminoacylation; IBA:GO_Central.
DR   CDD; cd00771; ThrRS_core; 1.
DR   Gene3D; 3.10.20.30; -; 1.
DR   Gene3D; 3.30.930.10; -; 1.
DR   Gene3D; 3.40.50.800; -; 1.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004154; Anticodon-bd.
DR   InterPro; IPR036621; Anticodon-bd_dom_sf.
DR   InterPro; IPR012675; Beta-grasp_dom_sf.
DR   InterPro; IPR004095; TGS.
DR   InterPro; IPR002320; Thr-tRNA-ligase_IIa.
DR   InterPro; IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
DR   InterPro; IPR033728; ThrRS_core.
DR   PANTHER; PTHR11451; PTHR11451; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   PRINTS; PR01047; TRNASYNTHTHR.
DR   SUPFAM; SSF55186; SSF55186; 1.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   TIGRFAMs; TIGR00418; thrS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
DR   PROSITE; PS51880; TGS; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..698
FT                   /note="Probable threonine--tRNA ligase 2, cytoplasmic"
FT                   /id="PRO_0000333832"
FT   DOMAIN          38..100
FT                   /note="TGS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01228"
FT   REGION          541..560
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   698 AA;  81318 MW;  C08DC679012694A1 CRC64;
     MSFLKLTKSI QKINNFNNNK INLINKYFST TNINNSGGGG NIKLNDGRIF KFEDKQTPLT
     IANKINKTIG KQSILSRLNG NKLISMKEII EMSGKDYNIE FLNFEEHSDA RICFWNSSSL
     VLARATMEYF KNENKEIELI NFGHLVNPQT ADNINQGTFY IDIFFKDKNQ TIKDNDINKI
     KKIMEYIVKR NDKFEILKND QQDQDQDQEE SFIKFGEFKF KNNFLTIDSS NSIVSLDLIK
     NSSVVGPNKE YSELQRIVGI SFPSKDQMNN WVEIQKIAAL RDHRVIGKDQ ELFFFHPFSP
     GSCFFLPHGT KIYNKLLQFL RLEYRKRGYQ EVISPNIYNQ KLWETSGHWD NYKDNMFSFE
     CDHTQYSLKP MNCPGHCLMY AHRARSYKEL PMRIADFGVL HRNETHGSLS GLTRVRRFQQ
     DDAHIFCTPD MIREEIKQCL DFMKYVYTIF NFTFHLELST RPDSYLGELS VWEKAETSLS
     QVLTEFCGDK WTINHGDGAF YGPKIDIHLK DANGKNHQCA TIQLDFQLPI RFNLEYSGGL
     NNNNNNNNNN EEINDNNNNN SLNRPVMIHR ALFGSVERMM AILMEHTAGK WPFWLSPRQC
     IVIPVSNKFN QFAQEIQSKI NLAGYDVDVD LNDSKLLSKK IREATVSQYN YIIVVGQEEI
     DTNILNVRKR DLPEDNKQVK LSLNDLFSEF KLNIEQFK