ID   SYTC_ARATH              Reviewed;         458 AA.
AC   Q8GZ45; Q9LMT4;
DT   24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 2.
DT   25-MAY-2022, entry version 124.
DE   RecName: Full=Probable threonine--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.3;
DE   AltName: Full=Threonyl-tRNA synthetase;
DE            Short=ThrRS;
GN   OrderedLocusNames=At1g17960; ORFNames=F2H15.18;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RX   PubMed=11910074; DOI=10.1126/science.1071006;
RA   Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA   Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA   Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA   Shinagawa A., Shinozaki K.;
RT   "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL   Science 296:141-145(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-
CC         threonyl-tRNA(Thr); Xref=Rhea:RHEA:24624, Rhea:RHEA-COMP:9670,
CC         Rhea:RHEA-COMP:9704, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57926, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78534, ChEBI:CHEBI:456215; EC=6.1.1.3;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q8GZ45-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8GZ45-2; Sequence=VSP_017125, VSP_017126;
CC   -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; AC034106; AAF97275.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE29656.1; -; Genomic_DNA.
DR   EMBL; AK117218; BAC41894.1; -; mRNA.
DR   PIR; H86314; H86314.
DR   RefSeq; NP_173238.1; NM_101659.3. [Q8GZ45-1]
DR   AlphaFoldDB; Q8GZ45; -.
DR   SMR; Q8GZ45; -.
DR   BioGRID; 23614; 13.
DR   STRING; 3702.AT1G17960.1; -.
DR   PaxDb; Q8GZ45; -.
DR   PRIDE; Q8GZ45; -.
DR   ProteomicsDB; 233037; -. [Q8GZ45-1]
DR   EnsemblPlants; AT1G17960.1; AT1G17960.1; AT1G17960. [Q8GZ45-1]
DR   GeneID; 838375; -.
DR   Gramene; AT1G17960.1; AT1G17960.1; AT1G17960. [Q8GZ45-1]
DR   KEGG; ath:AT1G17960; -.
DR   Araport; AT1G17960; -.
DR   TAIR; locus:2030918; AT1G17960.
DR   eggNOG; KOG1637; Eukaryota.
DR   HOGENOM; CLU_008554_2_1_1; -.
DR   InParanoid; Q8GZ45; -.
DR   OMA; WHSSALI; -.
DR   OrthoDB; 813937at2759; -.
DR   PhylomeDB; Q8GZ45; -.
DR   PRO; PR:Q8GZ45; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q8GZ45; baseline and differential.
DR   Genevisible; Q8GZ45; AT.
DR   GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004829; F:threonine-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0006435; P:threonyl-tRNA aminoacylation; IBA:GO_Central.
DR   Gene3D; 3.10.20.30; -; 1.
DR   Gene3D; 3.30.930.10; -; 1.
DR   Gene3D; 3.40.50.800; -; 1.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004154; Anticodon-bd.
DR   InterPro; IPR036621; Anticodon-bd_dom_sf.
DR   InterPro; IPR012675; Beta-grasp_dom_sf.
DR   InterPro; IPR004095; TGS.
DR   InterPro; IPR012676; TGS-like.
DR   InterPro; IPR002320; Thr-tRNA-ligase_IIa.
DR   InterPro; IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
DR   PANTHER; PTHR11451; PTHR11451; 2.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF02824; TGS; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   PRINTS; PR01047; TRNASYNTHTHR.
DR   SUPFAM; SSF55186; SSF55186; 1.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   SUPFAM; SSF81271; SSF81271; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
DR   PROSITE; PS51880; TGS; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm;
KW   Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..458
FT                   /note="Probable threonine--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000101123"
FT   DOMAIN          41..104
FT                   /note="TGS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01228"
FT   VAR_SEQ         169..186
FT                   /note="KLPIRMAEFGVLHRNEDS -> SVAYISFADIFFVLRYGM (in isoform
FT                   2)"
FT                   /evidence="ECO:0000303|PubMed:11910074"
FT                   /id="VSP_017125"
FT   VAR_SEQ         187..458
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:11910074"
FT                   /id="VSP_017126"
SQ   SEQUENCE   458 AA;  52436 MW;  BBFBC833E5AE0608 CRC64;
     MANNHHPKDE AYLSSVIPKR IRLFEEIQAE QLEQLQSRPH DPIKITLLPD GIEKEGRRWE
     TSPMDIAVQI SKGLAKSALV SSVNHVLWDM NRPLEGDCSL EIFGFDSDQG RNTFWHSSAH
     ILGQALEQEY GCKLCIGPCE PRDEGFYYDS LYYGELGLND NHFPNIEAKL PIRMAEFGVL
     HRNEDSGALG GMTRVRRFVQ DDAHIFCRVD QVEEEVKGVL DFIDYVYRIF GFTYELTLST
     RPKDHIGDLE TWAKAENDLE KALDDFGKPW VIKEGDGAFY GPKIDITVSD ARNRKFQCAT
     IQLDFQLPAC FKLKYLSEKN EMEAPVMIHS AVLGSIERMF AILLEHYKGI WPFWLSPRQA
     IVCSLSEDCS SYAKQVQKQI NEVGYYVDID ESDRSLRKKV ADARDAPYNY ILVVGQKEVA
     TGQVTVRLRE DPEGRKDLPE MSIESLLDEF KFKTVNFL