ID   SYTC_CAEEL              Reviewed;         725 AA.
AC   P52709;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 156.
DE   RecName: Full=Threonine--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.3;
DE   AltName: Full=Threonyl-tRNA synthetase;
DE            Short=ThrRS;
GN   Name=tars-1 {ECO:0000312|WormBase:C47D12.6a};
GN   Synonyms=trs-1 {ECO:0000312|WormBase:C47D12.6a}; ORFNames=C47D12.6;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-
CC         threonyl-tRNA(Thr); Xref=Rhea:RHEA:24624, Rhea:RHEA-COMP:9670,
CC         Rhea:RHEA-COMP:9704, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57926, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78534, ChEBI:CHEBI:456215; EC=6.1.1.3;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; Z69902; CAA93762.1; -; Genomic_DNA.
DR   PIR; T19994; T19994.
DR   RefSeq; NP_001022033.1; NM_001026862.3.
DR   AlphaFoldDB; P52709; -.
DR   SMR; P52709; -.
DR   BioGRID; 40095; 3.
DR   DIP; DIP-24299N; -.
DR   STRING; 6239.C47D12.6a; -.
DR   EPD; P52709; -.
DR   PaxDb; P52709; -.
DR   PeptideAtlas; P52709; -.
DR   EnsemblMetazoa; C47D12.6a.1; C47D12.6a.1; WBGene00006617.
DR   GeneID; 174790; -.
DR   KEGG; cel:CELE_C47D12.6; -.
DR   UCSC; C47D12.6b.1; c. elegans.
DR   CTD; 174790; -.
DR   WormBase; C47D12.6a; CE05434; WBGene00006617; tars-1.
DR   eggNOG; KOG1637; Eukaryota.
DR   InParanoid; P52709; -.
DR   OMA; IWDEAEK; -.
DR   OrthoDB; 813937at2759; -.
DR   PhylomeDB; P52709; -.
DR   PRO; PR:P52709; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00006617; Expressed in adult organism and 4 other tissues.
DR   ExpressionAtlas; P52709; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004829; F:threonine-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0008340; P:determination of adult lifespan; IMP:WormBase.
DR   GO; GO:0006435; P:threonyl-tRNA aminoacylation; IBA:GO_Central.
DR   CDD; cd00771; ThrRS_core; 1.
DR   Gene3D; 3.10.20.30; -; 1.
DR   Gene3D; 3.30.930.10; -; 1.
DR   Gene3D; 3.40.50.800; -; 1.
DR   HAMAP; MF_00184; Thr_tRNA_synth; 1.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004154; Anticodon-bd.
DR   InterPro; IPR036621; Anticodon-bd_dom_sf.
DR   InterPro; IPR012675; Beta-grasp_dom_sf.
DR   InterPro; IPR004095; TGS.
DR   InterPro; IPR012676; TGS-like.
DR   InterPro; IPR002320; Thr-tRNA-ligase_IIa.
DR   InterPro; IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
DR   InterPro; IPR033728; ThrRS_core.
DR   InterPro; IPR012947; tRNA_SAD.
DR   PANTHER; PTHR11451; PTHR11451; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF02824; TGS; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   Pfam; PF07973; tRNA_SAD; 1.
DR   PRINTS; PR01047; TRNASYNTHTHR.
DR   SMART; SM00863; tRNA_SAD; 1.
DR   SUPFAM; SSF55186; SSF55186; 1.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   SUPFAM; SSF81271; SSF81271; 1.
DR   TIGRFAMs; TIGR00418; thrS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
DR   PROSITE; PS51880; TGS; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..725
FT                   /note="Threonine--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000101122"
FT   DOMAIN          80..142
FT                   /note="TGS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01228"
SQ   SEQUENCE   725 AA;  84417 MW;  C234CCB61CDF5BDA CRC64;
     MRLNCFRIFV HIQKPTQIFK PFYRSLSSEA SDKYHFVNGH KMSKAPTDMA PWPAFIEERI
     KLWDKLKAEY DAEIAAKESE PIQITLPDGK IHEGKTWRTT PFEIAERISK GLAEAAVIAK
     VNGAVWDLDR PFEGNAKLEL LKFDDDEAKQ VFWHSSAHVL GEAMERYCGG HLCYGPPIQE
     GFYYDMWHEN RTICPDDFPK IDQIVKAAVK DKQKFERLEM TKEDLLEMFK YNEFKVRIIT
     EKIHTPKTTV YRCGPLIDLC RGPHVRHTGK VKAMAITKNS SSYWEGKADA ESLQRLYGIS
     FPDSKQLKEW QKLQEEAAKR DHRKLGKEHD LFFFHQLSPG SAFWYPKGAH IYNKLVDFIR
     KQYRRRGFTE VITPNMYNKK LWETSGHWQH YSEDMFKIEV EKEEFGLKPM NCPGHCLMFG
     HMPHTYNELP FRFADFGVLH RNEMSGALTG LTRVRRFQQD DAHIFCRQDQ ISEEIKQCLD
     FLEYAYEKVF GFTFKLNLST RPEGFLGNIE TWDKAEADLT NALNASGRKW VLNPGDGAFY
     GPKIDITIQD ALKRNFQCAT IQLDFQLPNQ FDLSYFDEKG EKQRPVMIHR AVLGSVERMT
     AILTESYGGK WPFWLSPRQC KIITVHESVR DYANDVKKQI FEAGFEIEYE ENCGDTMNKQ
     VRKAQLAQFN FILVIGAKEK ENGTVNVRTR DNAVRGEVAL DKLISKFRRF ADEYVADTEK
     SEEWA