ID   SYTC_ENTBH              Reviewed;         634 AA.
AC   B7XIA1;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Probable threonine--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.3;
DE   AltName: Full=Threonyl-tRNA synthetase;
DE            Short=ThrRS;
GN   ORFNames=EBI_22768;
OS   Enterocytozoon bieneusi (strain H348) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Enterocytozoonidae;
OC   Enterocytozoon.
OX   NCBI_TaxID=481877;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H348;
RX   PubMed=18060071; DOI=10.1371/journal.pone.0001277;
RA   Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S.,
RA   Keeling P.J.;
RT   "Patterns of genome evolution among the microsporidian parasites
RT   Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon
RT   bieneusi.";
RL   PLoS ONE 2:E1277-E1277(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H348;
RX   PubMed=19132089; DOI=10.1371/journal.ppat.1000261;
RA   Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N.,
RA   Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S.;
RT   "Genomic survey of the non-cultivatable opportunistic human pathogen,
RT   Enterocytozoon bieneusi.";
RL   PLoS Pathog. 5:E1000261-E1000261(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-
CC         threonyl-tRNA(Thr); Xref=Rhea:RHEA:24624, Rhea:RHEA-COMP:9670,
CC         Rhea:RHEA-COMP:9704, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57926, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78534, ChEBI:CHEBI:456215; EC=6.1.1.3;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; ABGB01000017; EED44394.1; -; Genomic_DNA.
DR   RefSeq; XP_002649729.1; XM_002649683.1.
DR   AlphaFoldDB; B7XIA1; -.
DR   SMR; B7XIA1; -.
DR   STRING; 481877.B7XIA1; -.
DR   PRIDE; B7XIA1; -.
DR   EnsemblFungi; EED44394; EED44394; EBI_22768.
DR   VEuPathDB; MicrosporidiaDB:EBI_22768; -.
DR   HOGENOM; CLU_008554_0_1_1; -.
DR   InParanoid; B7XIA1; -.
DR   Proteomes; UP000001742; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004829; F:threonine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006435; P:threonyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd00771; ThrRS_core; 1.
DR   Gene3D; 3.30.930.10; -; 1.
DR   Gene3D; 3.40.50.800; -; 1.
DR   HAMAP; MF_00184; Thr_tRNA_synth; 1.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004154; Anticodon-bd.
DR   InterPro; IPR036621; Anticodon-bd_dom_sf.
DR   InterPro; IPR004095; TGS.
DR   InterPro; IPR002320; Thr-tRNA-ligase_IIa.
DR   InterPro; IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
DR   InterPro; IPR033728; ThrRS_core.
DR   InterPro; IPR012947; tRNA_SAD.
DR   PANTHER; PTHR11451; PTHR11451; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   Pfam; PF07973; tRNA_SAD; 1.
DR   PRINTS; PR01047; TRNASYNTHTHR.
DR   SMART; SM00863; tRNA_SAD; 1.
DR   SUPFAM; SSF55186; SSF55186; 1.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   TIGRFAMs; TIGR00418; thrS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
DR   PROSITE; PS51880; TGS; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..634
FT                   /note="Probable threonine--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000388385"
FT   DOMAIN          1..61
FT                   /note="TGS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01228"
SQ   SEQUENCE   634 AA;  74069 MW;  D4209979C67FB8E1 CRC64;
     MSIYVTFKGQ VQKICISSGT ILQELISTHY PNKNIIACAI DGIMADINTE INENQKIELY
     DFDSKEGQHV YWHSSAHILG NALVNLYQCK LVNGPALDEG FYYDIDIERP IREEDFADIE
     KEMQRIINKN IDFRKKMIKK DDLLKMYKDN DYKVHFINNI PDNLISVYYN DEFYDMCQGP
     HIQSTGLVKA FKILKSSSCY FLNSADNQIL QRIYGISFPN KTLLKEYEEK VQKAKEMDHR
     KIGKELNLYF FHEYSPGSCF WLPDGVVIYN RLMEFLRKEY IKRGFKEVIT PNIFHIDLWK
     ESGHYQNYKE NIYGISGEDF ALKPMNCPGH CIIFKSVERS YKELPLRYAD FGVLHRNECS
     GSLTGLTRVR RFQQDDAHIF TSKSSIKEEI ENAIGFLKTV YSIFNFKYEL FLSTRPTKFL
     GTIEEWDVAE KSLKDAIIDS GHTYTLNEGD GAFYGPKIDI ILHDILNRKI QCATIQLDFQ
     LPQRFDLKFK NELGMYETPV IIHRAILGSF ERFIAILIES YGKHLPFWLH PRQVGLVTID
     CKHENYMYTV EKSMLNVVLD LGIRKYTDPK DTLNKKIRKA TLDGCKIICI LGDKEMEKNE
     VNVRIGNKTR TYKIEELLEK IKVSIETKKE FIFN