SYTL5_MOUSE
ID SYTL5_MOUSE Reviewed; 753 AA.
AC Q80T23; A2AF59; Q812E5;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Synaptotagmin-like protein 5;
GN Name=Sytl5; Synonyms=Slp5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND INTERACTION WITH RAB27A.
RC STRAIN=BALB/cJ; TISSUE=Spleen, and Testis;
RX PubMed=12590134; DOI=10.1074/jbc.m213090200;
RA Fukuda M.;
RT "Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through
RT interaction with the GDP-bound form of Rab27A in PC12 cells.";
RL J. Biol. Chem. 278:15390-15396(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-147, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May act as Rab effector protein and play a role in vesicle
CC trafficking. Binds phospholipids (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Binds RAB27A that has been activated by GTP-binding.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q80T23-1; Sequence=Displayed;
CC Name=2; Synonyms=Slp5 delta 5S-I;
CC IsoId=Q80T23-2; Sequence=VSP_007905;
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DR EMBL; AB098160; BAC57421.1; -; mRNA.
DR EMBL; AB098161; BAC57422.1; -; mRNA.
DR EMBL; AL672031; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS40860.1; -. [Q80T23-1]
DR CCDS; CCDS72342.1; -. [Q80T23-2]
DR RefSeq; NP_001277657.1; NM_001290728.1. [Q80T23-2]
DR RefSeq; NP_808372.1; NM_177704.3. [Q80T23-1]
DR RefSeq; XP_006527667.1; XM_006527604.3. [Q80T23-1]
DR RefSeq; XP_006527670.1; XM_006527607.3. [Q80T23-1]
DR RefSeq; XP_006527671.1; XM_006527608.3. [Q80T23-1]
DR RefSeq; XP_011245762.1; XM_011247460.2. [Q80T23-1]
DR RefSeq; XP_011245765.1; XM_011247463.2. [Q80T23-1]
DR RefSeq; XP_011245766.1; XM_011247464.1. [Q80T23-1]
DR RefSeq; XP_011245767.1; XM_011247465.1. [Q80T23-1]
DR RefSeq; XP_011245770.1; XM_011247468.1. [Q80T23-1]
DR RefSeq; XP_011245771.1; XM_011247469.2. [Q80T23-1]
DR RefSeq; XP_011245772.1; XM_011247470.2. [Q80T23-1]
DR RefSeq; XP_011245773.1; XM_011247471.2. [Q80T23-1]
DR RefSeq; XP_011245775.1; XM_011247473.1. [Q80T23-2]
DR RefSeq; XP_017173956.1; XM_017318467.1. [Q80T23-1]
DR AlphaFoldDB; Q80T23; -.
DR SMR; Q80T23; -.
DR BioGRID; 231780; 1.
DR CORUM; Q80T23; -.
DR IntAct; Q80T23; 1.
DR STRING; 10090.ENSMUSP00000064826; -.
DR iPTMnet; Q80T23; -.
DR PhosphoSitePlus; Q80T23; -.
DR MaxQB; Q80T23; -.
DR PaxDb; Q80T23; -.
DR PRIDE; Q80T23; -.
DR ProteomicsDB; 253449; -. [Q80T23-1]
DR ProteomicsDB; 253450; -. [Q80T23-2]
DR Antibodypedia; 10579; 98 antibodies from 23 providers.
DR DNASU; 236643; -.
DR Ensembl; ENSMUST00000067529; ENSMUSP00000064826; ENSMUSG00000054453. [Q80T23-1]
DR Ensembl; ENSMUST00000086165; ENSMUSP00000083339; ENSMUSG00000054453. [Q80T23-2]
DR GeneID; 236643; -.
DR KEGG; mmu:236643; -.
DR UCSC; uc009sqa.2; mouse. [Q80T23-1]
DR UCSC; uc009sqb.2; mouse. [Q80T23-2]
DR CTD; 94122; -.
DR MGI; MGI:2668451; Sytl5.
DR VEuPathDB; HostDB:ENSMUSG00000054453; -.
DR eggNOG; KOG1028; Eukaryota.
DR GeneTree; ENSGT00940000158618; -.
DR HOGENOM; CLU_002711_5_0_1; -.
DR InParanoid; Q80T23; -.
DR OMA; GHWKCSV; -.
DR PhylomeDB; Q80T23; -.
DR TreeFam; TF341184; -.
DR BioGRID-ORCS; 236643; 0 hits in 75 CRISPR screens.
DR ChiTaRS; Sytl5; mouse.
DR PRO; PR:Q80T23; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q80T23; protein.
DR Bgee; ENSMUSG00000054453; Expressed in uterus and 29 other tissues.
DR ExpressionAtlas; Q80T23; baseline and differential.
DR GO; GO:0070382; C:exocytic vesicle; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042043; F:neurexin family protein binding; IBA:GO_Central.
DR GO; GO:0005543; F:phospholipid binding; IEA:InterPro.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0006887; P:exocytosis; IDA:MGI.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR CDD; cd04029; C2A_SLP-4_5; 1.
DR CDD; cd04020; C2B_SLP_1-2-3-4; 1.
DR CDD; cd15766; FYVE_Slp5; 1.
DR Gene3D; 2.60.40.150; -; 2.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR041282; FYVE_2.
DR InterPro; IPR010911; Rab_BD.
DR InterPro; IPR037303; SLP-4/5_C2A.
DR InterPro; IPR028702; Slp5.
DR InterPro; IPR043567; SYTL1-5_C2B.
DR InterPro; IPR042783; SYTL5_FYVE.
DR InterPro; IPR011011; Znf_FYVE_PHD.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR45716:SF6; PTHR45716:SF6; 1.
DR Pfam; PF00168; C2; 2.
DR Pfam; PF02318; FYVE_2; 1.
DR SMART; SM00239; C2; 2.
DR SUPFAM; SSF49562; SSF49562; 2.
DR SUPFAM; SSF57903; SSF57903; 1.
DR PROSITE; PS50004; C2; 2.
DR PROSITE; PS50916; RABBD; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Metal-binding; Phosphoprotein;
KW Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..753
FT /note="Synaptotagmin-like protein 5"
FT /id="PRO_0000190220"
FT DOMAIN 7..123
FT /note="RabBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00234"
FT DOMAIN 429..550
FT /note="C2 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 597..717
FT /note="C2 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT ZN_FING 64..106
FT /note="FYVE-type"
FT REGION 145..279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 297..359
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 380..404
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 146..175
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 210..279
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 303..323
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 324..342
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 380..395
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 147
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 387..408
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12590134"
FT /id="VSP_007905"
SQ SEQUENCE 753 AA; 84089 MW; C1560AB677818B57 CRC64;
MSKNSEFINL SFLLDHEKEM ILGVLKRDEY LKKVEDKRIR KLKNELLEAK RRSGKTHQEA
SRVCVHCHKT LGLIFDRGDP CQACSLRVCS ECRVTGLDGS WKCTVCAKVA QLRIISGEWF
LEEKAKRFKQ VNVLGTDVVR QSILRRSPGS EETQNQEQAQ QCVDKSDTLS SVRQKTTHDG
PKKKGFLLSK FRSATRGEIR TPKPESGRSY SLDLDSQNLQ SFKSASGSDR GSTTSSDLID
QEAGRRTSKS SYSNGGIPVT QRSPVPSAHS VTSINSREHG FENSMALATI ENTCEELTKS
HRRNTSGTPS IAVSGTSLSS ERSRSEVDLS ESFAEDLEDT SSIRSRSVPG ALDKDLNSLE
DTEDGVDLVS SRFSANTHSL ASGLSTNSQA GSDRKRSYLN VPDADSDTTS LNSMMSVYSE
TGDYGNVKVT GEILLHISYC YKTGGLYIFV KSCRNLATGD EKKQRTDAYV KSYLLPDKSR
NNKRKTKIRT GTNPEFNETL KYTISHTQLE TRTLQLSVWH YDRFGRNSFL GEVEIAFDSW
NFENPCDEWF VLQPKVELAP DISLQYKGEL TIVLRYIPPE ENLIFPAGQR QEKKIFKRGK
KKESSSISGG ILEVFIKKAK NLTAVKSGGT SDSFVKGYLL PDDNKATKHK TAVVKKSVNP
EWNHTFIFSG LYPQDIKNAC LELTIWDKEA FSSNVFLGGV RLNSGSGMSY GKTVDWMDSH
GEEQRLWQKM ADNPGTSVEG VLMLRSSMAK CRL
