ID   BOMB_RANSH              Reviewed;          67 AA.
AC   P86994;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Bombesin {ECO:0000303|PubMed:21104135};
DE   AltName: Full=Bombesin-RS {ECO:0000303|PubMed:21104135};
DE   Flags: Precursor;
OS   Rana shuchinae (Sichuan frog) (Pelophylax shuchinae).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Rana; Rana.
OX   NCBI_TaxID=359668;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 50-64, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PYROGLUTAMATE FORMATION AT
RP   GLN-50, AMIDATION AT MET-64, SYNTHESIS, AND MASS SPECTROMETRY.
RC   TISSUE=Skin {ECO:0000269|PubMed:21104135}, and
RC   Skin secretion {ECO:0000269|PubMed:21104135};
RX   PubMed=21104135; DOI=10.1007/s11033-010-0471-x;
RA   Wang H., Bian J., Chen Z., Miao Y., Li W.;
RT   "A novel bombesin-like peptide from skin of Rana shuchinae.";
RL   Mol. Biol. Rep. 38:3599-3603(2011).
CC   -!- FUNCTION: Stimulates smooth muscle contraction in isolated rat stomach
CC       strip. {ECO:0000269|PubMed:21104135}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21104135}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:21104135}.
CC   -!- MASS SPECTROMETRY: Mass=1659.1; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:21104135};
CC   -!- SIMILARITY: Belongs to the bombesin/neuromedin-B/ranatensin family.
CC       {ECO:0000255}.
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DR   AlphaFoldDB; P86994; -.
DR   SMR; P86994; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:InterPro.
DR   InterPro; IPR000874; Bombesin.
DR   Pfam; PF02044; Bombesin; 1.
DR   PROSITE; PS00257; BOMBESIN; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Pyrrolidone carboxylic acid; Secreted; Signal.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000255"
FT   PROPEP          31..49
FT                   /evidence="ECO:0000255, ECO:0000269|PubMed:21104135"
FT                   /id="PRO_0000413628"
FT   PEPTIDE         50..64
FT                   /note="Bombesin"
FT                   /evidence="ECO:0000269|PubMed:21104135"
FT                   /id="PRO_0000413629"
FT   MOD_RES         50
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:21104135"
FT   MOD_RES         64
FT                   /note="Methionine amide"
FT                   /evidence="ECO:0000269|PubMed:21104135"
SQ   SEQUENCE   67 AA;  7441 MW;  E0D8C25C02D1C8F9 CRC64;
     MLLLSAVKTL LLAWLGIVLV FMSIIKSAML DFLQEAGKLE GIETYKKEAQ TSFMAPSWAL
     GHLMGRK