ID   BOPE_BURM7              Reviewed;         261 AA.
AC   A3MCH6;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Guanine nucleotide exchange factor BopE;
DE   AltName: Full=Effector protein BopE;
GN   Name=bopE; OrderedLocusNames=BMA10247_A0761;
OS   Burkholderia mallei (strain NCTC 10247).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=320389;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 10247;
RX   PubMed=20333227; DOI=10.1093/gbe/evq003;
RA   Losada L., Ronning C.M., DeShazer D., Woods D., Fedorova N., Kim H.S.,
RA   Shabalina S.A., Pearson T.R., Brinkac L., Tan P., Nandi T., Crabtree J.,
RA   Badger J., Beckstrom-Sternberg S., Saqib M., Schutzer S.E., Keim P.,
RA   Nierman W.C.;
RT   "Continuing evolution of Burkholderia mallei through genome reduction and
RT   large-scale rearrangements.";
RL   Genome Biol. Evol. 2:102-116(2010).
CC   -!- FUNCTION: Activator for both CDC42 and RAC1 by directly interacting
CC       with these Rho GTPases and acting as a guanine nucleotide exchange
CC       factor (GEF). This activation results in actin cytoskeleton
CC       rearrangements and stimulates membrane ruffling, thus promoting
CC       bacterial entry into non-phagocytic cells (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Monomer. Interacts with human CDC42 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Note=Secreted via the bsa
CC       type III secretion system. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GEF (guanine exchange factor) SopE family.
CC       {ECO:0000305}.
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DR   EMBL; CP000547; ABO03206.1; -; Genomic_DNA.
DR   RefSeq; WP_004188462.1; NZ_CP007801.1.
DR   AlphaFoldDB; A3MCH6; -.
DR   BMRB; A3MCH6; -.
DR   SMR; A3MCH6; -.
DR   GeneID; 56597870; -.
DR   KEGG; bmaz:BM44_4985; -.
DR   KEGG; bmn:BMA10247_A0761; -.
DR   PATRIC; fig|320389.8.peg.5721; -.
DR   OMA; RQAIRHF; -.
DR   Proteomes; UP000002284; Chromosome II.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0031532; P:actin cytoskeleton reorganization; IEA:InterPro.
DR   GO; GO:0090630; P:activation of GTPase activity; IEA:InterPro.
DR   Gene3D; 1.10.4120.10; -; 1.
DR   InterPro; IPR005414; SopE.
DR   InterPro; IPR035949; SopE-like_GEF_dom_sf.
DR   InterPro; IPR016019; SopE_GEF_dom.
DR   Pfam; PF07487; SopE_GEF; 1.
DR   PIRSF; PIRSF034781; SecIII_sopE; 1.
DR   PRINTS; PR01593; SOPEPROTEIN.
DR   SUPFAM; SSF81832; SSF81832; 1.
PE   3: Inferred from homology;
KW   GTPase activation; Guanine-nucleotide releasing factor; Secreted;
KW   Virulence.
FT   CHAIN           1..261
FT                   /note="Guanine nucleotide exchange factor BopE"
FT                   /id="PRO_0000344029"
SQ   SEQUENCE   261 AA;  28735 MW;  B54EBF0C5A687D51 CRC64;
     MTYNPRIGGF THVKQASFDV HVKRGEAQPR TSFAQQIKRI FSKIGETLGQ LFRHRAPDSA
     PGRVRLQGVR YVGSYRPTGD AKQAIRHFVD EAVKQVAHTR TPEIRQDAEF GRQVYEATLC
     AIFSEAKDRF CMDPATRAGN VRPAFIEALG DAARATGLPG ADKQGVFTPS GAGTNPLYTE
     IRLRADTLMG AELAARPEYR ELQPYARQQA IDLVANALPA ERSNTLVEFR QTVQTLEATY
     RRAAQDASRD EKGATNAADG A