ID   BOPE_BURMA              Reviewed;         261 AA.
AC   Q62B14;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Guanine nucleotide exchange factor BopE;
DE   AltName: Full=Effector protein BopE;
GN   Name=bopE; OrderedLocusNames=BMAA1523;
OS   Burkholderia mallei (strain ATCC 23344).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=243160;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23344;
RX   PubMed=15377793; DOI=10.1073/pnas.0403306101;
RA   Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E.,
RA   Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C.,
RA   Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S.,
RA   Gwinn M.L., Haft D.H., Khouri H.M., Kolonay J.F., Madupu R., Mohammoud Y.,
RA   Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C.,
RA   Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M.;
RT   "Structural flexibility in the Burkholderia mallei genome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004).
CC   -!- FUNCTION: Activator for both CDC42 and RAC1 by directly interacting
CC       with these Rho GTPases and acting as a guanine nucleotide exchange
CC       factor (GEF). This activation results in actin cytoskeleton
CC       rearrangements and stimulates membrane ruffling, thus promoting
CC       bacterial entry into non-phagocytic cells (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Monomer. Interacts with human CDC42 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Note=Secreted via the bsa
CC       type III secretion system. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GEF (guanine exchange factor) SopE family.
CC       {ECO:0000305}.
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DR   EMBL; CP000011; AAU46021.1; -; Genomic_DNA.
DR   RefSeq; WP_004188462.1; NC_006349.2.
DR   RefSeq; YP_106114.1; NC_006349.2.
DR   AlphaFoldDB; Q62B14; -.
DR   SMR; Q62B14; -.
DR   STRING; 243160.BMAA1523; -.
DR   EnsemblBacteria; AAU46021; AAU46021; BMAA1523.
DR   GeneID; 56597870; -.
DR   KEGG; bma:BMAA1523; -.
DR   PATRIC; fig|243160.12.peg.5099; -.
DR   eggNOG; ENOG5033HJR; Bacteria.
DR   HOGENOM; CLU_1064256_0_0_4; -.
DR   OMA; RQAIRHF; -.
DR   Proteomes; UP000006693; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0031532; P:actin cytoskeleton reorganization; IEA:InterPro.
DR   GO; GO:0090630; P:activation of GTPase activity; IEA:InterPro.
DR   Gene3D; 1.10.4120.10; -; 1.
DR   InterPro; IPR005414; SopE.
DR   InterPro; IPR035949; SopE-like_GEF_dom_sf.
DR   InterPro; IPR016019; SopE_GEF_dom.
DR   Pfam; PF07487; SopE_GEF; 1.
DR   PIRSF; PIRSF034781; SecIII_sopE; 1.
DR   PRINTS; PR01593; SOPEPROTEIN.
DR   SUPFAM; SSF81832; SSF81832; 1.
PE   3: Inferred from homology;
KW   GTPase activation; Guanine-nucleotide releasing factor; Secreted;
KW   Virulence.
FT   CHAIN           1..261
FT                   /note="Guanine nucleotide exchange factor BopE"
FT                   /id="PRO_0000344027"
SQ   SEQUENCE   261 AA;  28735 MW;  B54EBF0C5A687D51 CRC64;
     MTYNPRIGGF THVKQASFDV HVKRGEAQPR TSFAQQIKRI FSKIGETLGQ LFRHRAPDSA
     PGRVRLQGVR YVGSYRPTGD AKQAIRHFVD EAVKQVAHTR TPEIRQDAEF GRQVYEATLC
     AIFSEAKDRF CMDPATRAGN VRPAFIEALG DAARATGLPG ADKQGVFTPS GAGTNPLYTE
     IRLRADTLMG AELAARPEYR ELQPYARQQA IDLVANALPA ERSNTLVEFR QTVQTLEATY
     RRAAQDASRD EKGATNAADG A