ID   SYVC_DICDI              Reviewed;        1072 AA.
AC   Q86KU2; Q54Z84;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Probable valine--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.9;
DE   AltName: Full=Valyl-tRNA synthetase;
DE            Short=ValRS;
GN   Name=valS1; ORFNames=DDB_G0277733;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-
CC         tRNA(Val); Xref=Rhea:RHEA:10704, Rhea:RHEA-COMP:9672, Rhea:RHEA-
CC         COMP:9708, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57762,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78537, ChEBI:CHEBI:456215; EC=6.1.1.9;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAFI02000022; EAL68540.1; -; Genomic_DNA.
DR   RefSeq; XP_642469.1; XM_637377.1.
DR   AlphaFoldDB; Q86KU2; -.
DR   SMR; Q86KU2; -.
DR   STRING; 44689.DDB0231269; -.
DR   PaxDb; Q86KU2; -.
DR   EnsemblProtists; EAL68540; EAL68540; DDB_G0277733.
DR   GeneID; 8621180; -.
DR   KEGG; ddi:DDB_G0277733; -.
DR   dictyBase; DDB_G0277733; valS1.
DR   eggNOG; KOG0432; Eukaryota.
DR   HOGENOM; CLU_001493_0_1_1; -.
DR   InParanoid; Q86KU2; -.
DR   OMA; FATKLWN; -.
DR   PhylomeDB; Q86KU2; -.
DR   PRO; PR:Q86KU2; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; ISS:dictyBase.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0031012; C:extracellular matrix; HDA:dictyBase.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004832; F:valine-tRNA ligase activity; ISS:dictyBase.
DR   GO; GO:0006438; P:valyl-tRNA aminoacylation; ISS:dictyBase.
DR   CDD; cd07962; Anticodon_Ia_Val; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_02004; Val_tRNA_synth_type1; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR033705; Anticodon_Ia_Val.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   InterPro; IPR002303; Valyl-tRNA_ligase.
DR   PANTHER; PTHR11946; PTHR11946; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00986; TRNASYNTHVAL.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00422; valS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Coiled coil; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..1072
FT                   /note="Probable valine--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000327760"
FT   REGION          1..79
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          10..90
FT                   /evidence="ECO:0000255"
FT   COILED          1003..1069
FT                   /evidence="ECO:0000255"
FT   MOTIF           151..161
FT                   /note="'HIGH' region"
FT                   /evidence="ECO:0000250"
FT   MOTIF           668..672
FT                   /note="'KMSKS' region"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        8..79
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         671
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   1072 AA;  122834 MW;  110221A2569A41A5 CRC64;
     MSEPKPTQPI KEIDEETKRK NEEKKKAKEA EKAEKLAKMK EKEAKQAAAK EKELQNKLKK
     EQEKKEKEAE EKEKQEAEEK IVREHIEKIL KTPKGEKKDV SSLLSSYHPT AVESIWYDYW
     LDNGYFSPEK QMEIQPHVVK DKKFVIVIPP PNVTGSLHLG HALTNSIQDA VVRYHRMKGE
     VCLWVPGTDH AGIATQVVVE KKIWKENKIT RHDLGREGFI KKVWEWKGEY GARIQGQLKK
     MGSSVDWSRE VFTMDDQRSK AVNTAFIRMF NDGLIVRTTR LVNWSCALKT AISDIEVDFI
     DLEKHTKMSV PGHQGQYDFG VLFEFAYQVE GSGEELVVAT TRIETMLADT AIAIHPDDTR
     YTHLHGKFAI HPLNGRKIPI ITDSVLVDKD FGTGVVKITP SHDPNDYETA LRHGLEFINL
     FTDEGLINEN GGERFAGMKR FDARNAVVEA LKEKNLFKGM KDNKMRLGLC SRSKDVIEPM
     IKPQWYVKCD DMAARAVKAV RDGDLKITPK THEVTWYRWL EGIKDWCVSR QLWWGHRIPA
     YHCIIKGVKS NPYDTNQWVV GANQEEATQN AIEKFKLSSK DDILSLEQDP DVLDTWFSSG
     LFPFSVMGWP EQTQDMQDYY PTSLLETGSD ILFFWVARMV MMGQQLTGKL PFEQVFLHAM
     VRDSHGRKMS KSLGNVIDPN DVIKGISLDE LIAKLYEGNL DSKEIEKATS GVKSDFPTGI
     AECGTDAMRF ALCAYTSQGR DINLDIQRVV GYRNFCNKIW NATRFAHMKL GDSFKPESFD
     ASKLLQQSNA INLWILNAAQ RAITLSEEGF KEYDFSKVTT AIYSFWLNEM CDVYLEMTKS
     IFSLEEDSPI KQKTKETLYT CIDIGLRLLH PFMPYLTEEL YQSLPRRPED VIPTIMLCPY
     PQSQSCWANS DIEEEMKQCQ DIIKSIRSLR ATYTIPASKK LHTYLHVKNQ ENLERFTKNS
     SFIRVLAYAS ELEVSISDES RPGCIVNVVN ENVSTLLDVR GSVDFNLEIA RLETKKQQLV
     KNYETLVSKT TIPSYDKVPQ NIRDDNTQKI KALDEEINIT NKTIESFTKL NI