BORA_DROME
ID BORA_DROME Reviewed; 539 AA.
AC Q9VVR2;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Protein aurora borealis;
GN Name=bora; ORFNames=CG6897;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, AND INTERACTION WITH AUR.
RX PubMed=16890155; DOI=10.1016/j.devcel.2006.06.002;
RA Hutterer A., Berdnik D., Wirtz-Peitz F., Zigman M., Schleiffer A.,
RA Knoblich J.A.;
RT "Mitotic activation of the kinase Aurora-A requires its binding partner
RT Bora.";
RL Dev. Cell 11:147-157(2006).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-383, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Required for the activation of Aurora-A (aur) at the onset of
CC mitosis. {ECO:0000269|PubMed:16890155}.
CC -!- SUBUNIT: Interacts with aur. {ECO:0000269|PubMed:16890155}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16890155}. Nucleus
CC {ECO:0000269|PubMed:16890155}. Note=Shuttles between the cytoplasm and
CC the nucleus. In interphase cells, it is nuclear. Upon entry into
CC mitosis, it is excluded from the nucleus and translocates into the
CC cytoplasm in a Cdk1-dependent manner.
CC -!- PTM: Phosphorylated by aur. {ECO:0000269|PubMed:16890155,
CC ECO:0000269|PubMed:18327897}.
CC -!- SIMILARITY: Belongs to the BORA family. {ECO:0000305}.
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DR EMBL; AE014296; AAF49248.1; -; Genomic_DNA.
DR EMBL; AY070548; AAL48019.1; -; mRNA.
DR RefSeq; NP_649048.1; NM_140791.3.
DR AlphaFoldDB; Q9VVR2; -.
DR BioGRID; 65312; 5.
DR IntAct; Q9VVR2; 3.
DR STRING; 7227.FBpp0074874; -.
DR iPTMnet; Q9VVR2; -.
DR PaxDb; Q9VVR2; -.
DR DNASU; 40031; -.
DR EnsemblMetazoa; FBtr0075108; FBpp0074874; FBgn0259791.
DR GeneID; 40031; -.
DR KEGG; dme:Dmel_CG6897; -.
DR CTD; 79866; -.
DR FlyBase; FBgn0259791; bora.
DR VEuPathDB; VectorBase:FBgn0259791; -.
DR eggNOG; ENOG502S85H; Eukaryota.
DR GeneTree; ENSGT00390000013790; -.
DR HOGENOM; CLU_464825_0_0_1; -.
DR InParanoid; Q9VVR2; -.
DR OMA; TQFEWNI; -.
DR OrthoDB; 1273292at2759; -.
DR PhylomeDB; Q9VVR2; -.
DR Reactome; R-DME-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR BioGRID-ORCS; 40031; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 40031; -.
DR PRO; PR:Q9VVR2; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0259791; Expressed in head epidermis primordium (Drosophila) and 39 other tissues.
DR Genevisible; Q9VVR2; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0032147; P:activation of protein kinase activity; IDA:UniProtKB.
DR GO; GO:0045167; P:asymmetric protein localization involved in cell fate determination; IMP:FlyBase.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007088; P:regulation of mitotic nuclear division; IBA:GO_Central.
DR GO; GO:0060236; P:regulation of mitotic spindle organization; IBA:GO_Central.
DR InterPro; IPR023252; Aurora_borealis_protein.
DR PANTHER; PTHR14728; PTHR14728; 1.
DR Pfam; PF15280; BORA_N; 1.
DR PRINTS; PR02038; AURORABORA.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Mitosis; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..539
FT /note="Protein aurora borealis"
FT /id="PRO_0000273210"
FT REGION 23..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 398..499
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..63
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 408..429
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 431..445
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 459..483
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 383
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 539 AA; 59142 MW; B5503F51B225CA02 CRC64;
MYNDEVRTPQ ALKNRYITNV NGLKCRARRN SHSNSSNSSA ASATPTNGGK ENGKYSPQMS
GNVCTPPPKR LHKVRNPFEG AMADRLHLPL IASPSLFRSR TPQLSSTQFE WNIDEVSQLK
PADVEPHETQ FHDSPDPEQE SKAQLAISAF FKESLIVPSP VDCPLRKQRI ILNCSEDNTP
ISNKSRRMRD CEVQTELTLP PILPKALEDA LRPYFQPHLA GRLSGRSKSS GGPDIFNSSM
RRKLFDLHNV IVLGEQDTAE PSRSMVGSSP QGKQTMFAGR LSDSASGESS FGCLSPIRNL
CGLPPGTPDN GTCSGKRKLL MHELELPSPI APSEHLSRRL VHSKVEISVT EQHDTLSERT
ALKFTPDRSS SPMGGGLEHS DCSINQRVRR LRVNSTRQVV IETGDQPLFE ETEGEEEEAE
SEDDEEADAM QLSTVSFNCS SSNSDTPRGH KRHRSAQRKN LSQSFSANLE EEADQTQGGA
GSIQPAEPPS VAMPQQGARI PLYRADSGFN ETSSTTFAFS QDLPLDVSMA CCSTPSTRS
