BORA_MOUSE
ID BORA_MOUSE Reviewed; 525 AA.
AC Q8BS90; Q3TJH7; Q3UWZ8; Q80WQ5; Q8C8E9;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Protein aurora borealis;
GN Name=Bora;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, Egg, Embryo, and Liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-305; SER-311 AND THR-331, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-316, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- FUNCTION: Required for the activation of AURKA at the onset of mitosis.
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with AURKA. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BS90-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BS90-2; Sequence=VSP_022496, VSP_022497;
CC -!- PTM: Phosphorylated by AURKA. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the BORA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC33015.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK034930; BAC28885.1; -; mRNA.
DR EMBL; AK047285; BAC33015.1; ALT_FRAME; mRNA.
DR EMBL; AK135995; BAE22766.1; -; mRNA.
DR EMBL; AK141389; BAE24669.1; -; mRNA.
DR EMBL; AK167430; BAE39518.1; -; mRNA.
DR EMBL; BC052183; AAH52183.1; -; mRNA.
DR CCDS; CCDS27309.1; -. [Q8BS90-1]
DR RefSeq; NP_780474.1; NM_175265.4. [Q8BS90-1]
DR AlphaFoldDB; Q8BS90; -.
DR STRING; 10090.ENSMUSP00000022656; -.
DR iPTMnet; Q8BS90; -.
DR PhosphoSitePlus; Q8BS90; -.
DR EPD; Q8BS90; -.
DR MaxQB; Q8BS90; -.
DR PaxDb; Q8BS90; -.
DR PeptideAtlas; Q8BS90; -.
DR PRIDE; Q8BS90; -.
DR ProteomicsDB; 273793; -. [Q8BS90-1]
DR ProteomicsDB; 273794; -. [Q8BS90-2]
DR Antibodypedia; 24385; 186 antibodies from 33 providers.
DR DNASU; 77744; -.
DR Ensembl; ENSMUST00000022656; ENSMUSP00000022656; ENSMUSG00000022070. [Q8BS90-1]
DR Ensembl; ENSMUST00000227744; ENSMUSP00000154660; ENSMUSG00000022070. [Q8BS90-2]
DR GeneID; 77744; -.
DR KEGG; mmu:77744; -.
DR UCSC; uc007uuv.1; mouse. [Q8BS90-1]
DR CTD; 79866; -.
DR MGI; MGI:1924994; Bora.
DR VEuPathDB; HostDB:ENSMUSG00000022070; -.
DR eggNOG; ENOG502S85H; Eukaryota.
DR GeneTree; ENSGT00390000013790; -.
DR HOGENOM; CLU_038376_0_0_1; -.
DR InParanoid; Q8BS90; -.
DR OMA; STWIKEP; -.
DR OrthoDB; 539473at2759; -.
DR PhylomeDB; Q8BS90; -.
DR TreeFam; TF329674; -.
DR Reactome; R-MMU-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR BioGRID-ORCS; 77744; 16 hits in 74 CRISPR screens.
DR ChiTaRS; Bora; mouse.
DR PRO; PR:Q8BS90; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8BS90; protein.
DR Bgee; ENSMUSG00000022070; Expressed in manus and 191 other tissues.
DR ExpressionAtlas; Q8BS90; baseline and differential.
DR Genevisible; Q8BS90; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0072687; C:meiotic spindle; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0019901; F:protein kinase binding; ISO:MGI.
DR GO; GO:0032147; P:activation of protein kinase activity; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007088; P:regulation of mitotic nuclear division; ISS:UniProtKB.
DR GO; GO:0060236; P:regulation of mitotic spindle organization; ISS:UniProtKB.
DR GO; GO:0032880; P:regulation of protein localization; ISS:UniProtKB.
DR InterPro; IPR023252; Aurora_borealis_protein.
DR PANTHER; PTHR14728; PTHR14728; 1.
DR Pfam; PF15280; BORA_N; 1.
DR PRINTS; PR02038; AURORABORA.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Methylation; Mitosis;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..525
FT /note="Protein aurora borealis"
FT /id="PRO_0000273206"
FT REGION 114..146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 505..525
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 119..142
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 191
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6PGQ7"
FT MOD_RES 250
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6PGQ7"
FT MOD_RES 305
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 311
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 316
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 331
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 271..273
FT /note="EPR -> GQI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_022496"
FT VAR_SEQ 274..525
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_022497"
FT CONFLICT 481
FT /note="Missing (in Ref. 1; BAE39518)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 525 AA; 57038 MW; 29CC9482494B96DE CRC64;
MGDVSELKMQ ITPETPGRIP VLNPFESPSD YSNLHEQTLA SPSIFKSTKL PTPGKFRWSI
DQLAIINPVE IDPEEIHRQA SYLRLSRIDK DVEDKRQKAI EEFFTKDVIV PSPWTDHDGK
QPSELHPSKC LSSHDDSPDG KKPSLPSQKC NAACQTLLSL PVDFNLEAIL GDYFREEDFV
AHTPGNLSSS SLRRKLFLDG NGSICDPLPS PSPGSPPCSA RGSLEGQFSS SPIQNSVKKY
SLGSVTTSPS AISSPTFSPI ALQGGKTPLS EPRKLTFHSP EASCATASTG IVNPSIRSPY
IDGCSPIKNW SPRRLRGGPQ CLSSLVRIPF TLEAHSEDEE ADVSCTGAAP LSTNACGEPR
VVTAMSVTQS HSGIAEKERA VLDDAESERE NDTVDMVDPT DTVAESTWIK EPVDDGNSPM
TDSASGIAFS IENSHMCMSP LAESSVLPYE SSAIQMNSDY NTQTCVSNIT DIVGTERYCK
ENVTHTNVPV PFEVEMKSQV NNVTPGHTAQ RCWMKSPRPS QCSRP
