BORC5_MOUSE
ID BORC5_MOUSE Reviewed; 195 AA.
AC Q9D920; Q8BJB0;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=BLOC-1-related complex subunit 5 {ECO:0000305};
GN Name=Borcs5 {ECO:0000312|MGI:MGI:1915024};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Bone marrow, Pancreas, Spleen, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Liver, Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: As part of the BORC complex may play a role in lysosomes
CC movement and localization at the cell periphery. Associated with the
CC cytosolic face of lysosomes, the BORC complex may recruit ARL8B and
CC couple lysosomes to microtubule plus-end-directed kinesin motor.
CC Thereby, it may indirectly play a role in cell spreading and motility.
CC {ECO:0000250|UniProtKB:Q969J3}.
CC -!- SUBUNIT: Component of the BLOC-one-related complex (BORC) which is
CC composed of BLOC1S1, BLOC1S2, BORCS5, BORCS6, BORCS7, BORCS8, KXD1 and
CC SNAPIN. Interacts with ARL8B, KIF5A, KLC1 and PLEKHM2; links the
CC lysosomal BORC complex to the microtubule plus-end-directed kinesin
CC motor. {ECO:0000250|UniProtKB:Q969J3}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:Q969J3};
CC Lipid-anchor {ECO:0000250|UniProtKB:Q969J3}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:Q969J3}.
CC -!- PTM: Myristoylation at Gly-2 mediates attachment to lysosome membranes.
CC {ECO:0000250|UniProtKB:Q969J3}.
CC -!- SIMILARITY: Belongs to the BORCS5 family. {ECO:0000305}.
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DR EMBL; AK007423; BAB25030.1; -; mRNA.
DR EMBL; AK089648; BAC40938.1; -; mRNA.
DR EMBL; AK151342; BAE30320.1; -; mRNA.
DR EMBL; AK169615; BAE41262.1; -; mRNA.
DR EMBL; BC023059; AAH23059.1; -; mRNA.
DR CCDS; CCDS20638.1; -.
DR RefSeq; NP_001163950.1; NM_001170479.1.
DR RefSeq; NP_080647.2; NM_026371.3.
DR AlphaFoldDB; Q9D920; -.
DR SMR; Q9D920; -.
DR BioGRID; 212434; 3.
DR ComplexPortal; CPX-5061; BORC complex.
DR STRING; 10090.ENSMUSP00000054913; -.
DR iPTMnet; Q9D920; -.
DR PhosphoSitePlus; Q9D920; -.
DR EPD; Q9D920; -.
DR MaxQB; Q9D920; -.
DR PaxDb; Q9D920; -.
DR PRIDE; Q9D920; -.
DR ProteomicsDB; 273695; -.
DR Antibodypedia; 23482; 160 antibodies from 21 providers.
DR DNASU; 67774; -.
DR Ensembl; ENSMUST00000062755; ENSMUSP00000054913; ENSMUSG00000042992.
DR GeneID; 67774; -.
DR KEGG; mmu:67774; -.
DR UCSC; uc012eul.1; mouse.
DR CTD; 118426; -.
DR MGI; MGI:1915024; Borcs5.
DR VEuPathDB; HostDB:ENSMUSG00000042992; -.
DR eggNOG; KOG4515; Eukaryota.
DR GeneTree; ENSGT00390000015016; -.
DR HOGENOM; CLU_064176_1_0_1; -.
DR InParanoid; Q9D920; -.
DR OMA; RFSEIPR; -.
DR OrthoDB; 1446665at2759; -.
DR TreeFam; TF316379; -.
DR BioGRID-ORCS; 67774; 2 hits in 40 CRISPR screens.
DR ChiTaRS; Borcs5; mouse.
DR PRO; PR:Q9D920; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9D920; protein.
DR Bgee; ENSMUSG00000042992; Expressed in animal zygote and 243 other tissues.
DR ExpressionAtlas; Q9D920; baseline and differential.
DR Genevisible; Q9D920; MM.
DR GO; GO:0099078; C:BORC complex; ISS:UniProtKB.
DR GO; GO:0098574; C:cytoplasmic side of lysosomal membrane; ISS:UniProtKB.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0031224; C:intrinsic component of membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0005873; C:plus-end kinesin complex; ISO:MGI.
DR GO; GO:0030672; C:synaptic vesicle membrane; IBA:GO_Central.
DR GO; GO:0032418; P:lysosome localization; ISS:UniProtKB.
DR GO; GO:0072384; P:organelle transport along microtubule; ISS:UniProtKB.
DR GO; GO:1903744; P:positive regulation of anterograde synaptic vesicle transport; IBA:GO_Central.
DR GO; GO:0051036; P:regulation of endosome size; IC:ComplexPortal.
DR GO; GO:0062196; P:regulation of lysosome size; IC:ComplexPortal.
DR InterPro; IPR018780; TBORCS5.
DR PANTHER; PTHR31634; PTHR31634; 1.
DR Pfam; PF10158; LOH1CR12; 1.
PE 1: Evidence at protein level;
KW Lipoprotein; Lysosome; Membrane; Myristate; Phosphoprotein;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q969J3"
FT CHAIN 2..195
FT /note="BLOC-1-related complex subunit 5"
FT /id="PRO_0000318597"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 71
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 75
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q969J3"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250|UniProtKB:Q969J3"
FT CONFLICT 58
FT /note="E -> K (in Ref. 1; BAC40938)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 195 AA; 22120 MW; F3DF9A7520D35D51 CRC64;
MGSEQSAEAE SRPGDLNASV TPSPAKHRAK MDDIVVVAQG SQASRNVSND PDVIKLQEIP
TFQPLLKGLL SGQTSPTNAK LEKLDSQQVL QLCLRYQDHL HQCAEAVAFD QNALVKRIKE
MDLSVETLFC FMQERQKRYA KYAEQIQKVN EMSAILRRIQ MGIDQTVPLM ERLNSMLPEA
ERLEPFSMKP ERERH
