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SYV_YEAST
ID   SYV_YEAST               Reviewed;        1104 AA.
AC   P07806; D6VUM6;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   03-AUG-2022, entry version 211.
DE   RecName: Full=Valine--tRNA ligase, mitochondrial;
DE            EC=6.1.1.9;
DE   AltName: Full=Valyl-tRNA synthetase;
DE            Short=ValRS;
DE   Flags: Precursor;
GN   Name=VAS1; OrderedLocusNames=YGR094W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3294828; DOI=10.1016/s0021-9258(18)48222-1;
RA   Jordana X., Chatton B., Paz-Weisshaar M., Buhler J.-M., Cramer F.,
RA   Ebel J.-P., Fasiolo F.;
RT   "Structure of the yeast valyl-tRNA synthetase gene (VASI) and the homology
RT   of its translated amino acid sequence with Escherichia coli isoleucyl-tRNA
RT   synthetase.";
RL   J. Biol. Chem. 262:7189-7194(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-78.
RX   PubMed=3275649; DOI=10.1016/s0021-9258(19)57354-9;
RA   Chatton B., Walter P., Ebel J.-P., Lacroute F., Fasiolo F.;
RT   "The yeast VAS1 gene encodes both mitochondrial and cytoplasmic valyl-tRNA
RT   synthetases.";
RL   J. Biol. Chem. 263:52-57(1988).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73; SER-294; SER-332; SER-707
RP   AND THR-1003, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP   ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-
CC         tRNA(Val); Xref=Rhea:RHEA:10704, Rhea:RHEA-COMP:9672, Rhea:RHEA-
CC         COMP:9708, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57762,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78537, ChEBI:CHEBI:456215; EC=6.1.1.9;
CC   -!- SUBCELLULAR LOCATION: [Isoform Cytoplasmic]: Cytoplasm.
CC   -!- SUBCELLULAR LOCATION: [Isoform Mitochondrial]: Mitochondrion.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=Mitochondrial;
CC         IsoId=P07806-1; Sequence=Displayed;
CC       Name=Cytoplasmic;
CC         IsoId=P07806-2; Sequence=VSP_018910;
CC   -!- MISCELLANEOUS: Present with 358 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- MISCELLANEOUS: [Isoform Cytoplasmic]: Produced by alternative
CC       initiation at Met-47 of isoform Mitochondrial. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; J02719; AAA35207.1; -; Genomic_DNA.
DR   EMBL; Z72879; CAA97097.1; -; Genomic_DNA.
DR   EMBL; M18392; AAA35205.1; -; Genomic_DNA.
DR   EMBL; M18392; AAA35206.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA08187.1; -; Genomic_DNA.
DR   PIR; S64389; SYBYVT.
DR   RefSeq; NP_011608.1; NM_001181223.1. [P07806-1]
DR   AlphaFoldDB; P07806; -.
DR   SMR; P07806; -.
DR   BioGRID; 33337; 89.
DR   DIP; DIP-6328N; -.
DR   IntAct; P07806; 18.
DR   MINT; P07806; -.
DR   STRING; 4932.YGR094W; -.
DR   iPTMnet; P07806; -.
DR   MaxQB; P07806; -.
DR   PaxDb; P07806; -.
DR   PRIDE; P07806; -.
DR   EnsemblFungi; YGR094W_mRNA; YGR094W; YGR094W. [P07806-1]
DR   GeneID; 852986; -.
DR   KEGG; sce:YGR094W; -.
DR   SGD; S000003326; VAS1.
DR   VEuPathDB; FungiDB:YGR094W; -.
DR   eggNOG; KOG0432; Eukaryota.
DR   GeneTree; ENSGT00940000167981; -.
DR   HOGENOM; CLU_001493_0_1_1; -.
DR   InParanoid; P07806; -.
DR   OMA; FATKLWN; -.
DR   BioCyc; YEAST:G3O-30804-MON; -.
DR   PRO; PR:P07806; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P07806; protein.
DR   GO; GO:0005737; C:cytoplasm; IMP:SGD.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; IMP:SGD.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004832; F:valine-tRNA ligase activity; IDA:SGD.
DR   GO; GO:0006438; P:valyl-tRNA aminoacylation; IDA:SGD.
DR   CDD; cd07962; Anticodon_Ia_Val; 1.
DR   Gene3D; 1.10.287.380; -; 1.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_02004; Val_tRNA_synth_type1; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR033705; Anticodon_Ia_Val.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR010978; tRNA-bd_arm.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR037118; Val-tRNA_synth_C_sf.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   InterPro; IPR002303; Valyl-tRNA_ligase.
DR   PANTHER; PTHR11946; PTHR11946; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00986; TRNASYNTHVAL.
DR   SUPFAM; SSF46589; SSF46589; 1.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00422; valS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   1: Evidence at protein level;
KW   Alternative initiation; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm;
KW   Ligase; Mitochondrion; Nucleotide-binding; Phosphoprotein;
KW   Protein biosynthesis; Reference proteome; Transit peptide.
FT   TRANSIT         1..47
FT                   /note="Mitochondrion"
FT   CHAIN           48..1104
FT                   /note="Valine--tRNA ligase, mitochondrial"
FT                   /id="PRO_0000035839"
FT   REGION          99..119
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           190..200
FT                   /note="'HIGH' region"
FT   MOTIF           703..707
FT                   /note="'KMSKS' region"
FT   BINDING         706
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         73
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   MOD_RES         294
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   MOD_RES         332
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   MOD_RES         707
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   MOD_RES         1003
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   VAR_SEQ         1..46
FT                   /note="Missing (in isoform Cytoplasmic)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018910"
FT   CONFLICT        147
FT                   /note="A -> G (in Ref. 1; AAA35207)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        540
FT                   /note="R -> K (in Ref. 1; AAA35207)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1104 AA;  125770 MW;  6493AEF37ECD4A7C CRC64;
     MNKWLNTLSK TFTFRLLNCH YRRSLPLCQN FSLKKSLTHN QVRFFKMSDL DNLPPVDPKT
     GEVIINPLKE DGSPKTPKEI EKEKKKAEKL LKFAAKQAKK NAAATTGASQ KKPKKKKEVE
     PIPEFIDKTV PGEKKILVSL DDPALKAYNP ANVESSWYDW WIKTGVFEPE FTADGKVKPE
     GVFCIPAPPP NVTGALHIGH ALTIAIQDSL IRYNRMKGKT VLFLPGFDHA GIATQSVVEK
     QIWAKDRKTR HDYGREAFVG KVWEWKEEYH SRIKNQIQKL GASYDWSREA FTLSPELTKS
     VEEAFVRLHD EGVIYRASRL VNWSVKLNTA ISNLEVENKD VKSRTLLSVP GYDEKVEFGV
     LTSFAYPVIG SDEKLIIATT RPETIFGDTA VAVHPDDDRY KHLHGKFIQH PFLPRKIPII
     TDKEAVDMEF GTGAVKITPA HDQNDYNTGK RHNLEFINIL TDDGLLNEEC GPEWQGMKRF
     DARKKVIEQL KEKNLYVGQE DNEMTIPTCS RSGDIIEPLL KPQWWVSQSE MAKDAIKVVR
     DGQITITPKS SEAEYFHWLG NIQDWCISRQ LWWGHRCPVY FINIEGEEHD RIDGDYWVAG
     RSMEEAEKKA AAKYPNSKFT LEQDEDVLDT WFSSGLWPFS TLGWPEKTKD METFYPFSML
     ETGWDILFFW VTRMILLGLK LTGSVPFKEV FCHSLVRDAQ GRKMSKSLGN VIDPLDVITG
     IKLDDLHAKL LQGNLDPREV EKAKIGQKES YPNGIPQCGT DAMRFALCAY TTGGRDINLD
     ILRVEGYRKF CNKIYQATKF ALMRLGDDYQ PPATEGLSGN ESLVEKWILH KLTETSKIVN
     EALDKRDFLT STSSIYEFWY LICDVYIENS KYLIQEGSAI EKKSAKDTLY ILLDNALKLI
     HPFMPFISEE MWQRLPKRST EKAASIVKAS YPVYVSEYDD VKSANAYDLV LNITKEARSL
     LSEYNILKNG KVFVESNHEE YFKTAEDQKD SIVSLIKAID EVTVVRDASE IPEGCVLQSV
     NPEVNVHLLV KGHVDIDAEI AKVQKKLEKA KKSKNGIEQT INSKDYETKA NTQAKEANKS
     KLDNTVAEIE GLEATIENLK RLKL
 
 
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