ID   SYW2_HALSA              Reviewed;         380 AA.
AC   Q9HN66;
DT   16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 111.
DE   RecName: Full=Tryptophan--tRNA ligase 2 {ECO:0000255|HAMAP-Rule:MF_00140};
DE            EC=6.1.1.2 {ECO:0000255|HAMAP-Rule:MF_00140};
DE   AltName: Full=Tryptophanyl-tRNA synthetase 2 {ECO:0000255|HAMAP-Rule:MF_00140};
DE            Short=TrpRS 2 {ECO:0000255|HAMAP-Rule:MF_00140};
GN   Name=trpS2 {ECO:0000255|HAMAP-Rule:MF_00140}; Synonyms=trpS;
GN   OrderedLocusNames=VNG_2232G;
OS   Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS   (Halobacterium halobium).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=64091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=11016950; DOI=10.1073/pnas.190337797;
RA   Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA   Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA   Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA   Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA   Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA   Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA   Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA   DasSarma S.;
RT   "Genome sequence of Halobacterium species NRC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-
CC         tryptophanyl-tRNA(Trp); Xref=Rhea:RHEA:24080, Rhea:RHEA-COMP:9671,
CC         Rhea:RHEA-COMP:9705, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57912, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78535, ChEBI:CHEBI:456215; EC=6.1.1.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00140};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00140}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00140}.
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DR   EMBL; AE004437; AAG20355.1; -; Genomic_DNA.
DR   PIR; G84373; G84373.
DR   RefSeq; WP_010903656.1; NC_002607.1.
DR   AlphaFoldDB; Q9HN66; -.
DR   SMR; Q9HN66; -.
DR   STRING; 64091.VNG_2232G; -.
DR   PaxDb; Q9HN66; -.
DR   PRIDE; Q9HN66; -.
DR   EnsemblBacteria; AAG20355; AAG20355; VNG_2232G.
DR   GeneID; 5953298; -.
DR   KEGG; hal:VNG_2232G; -.
DR   PATRIC; fig|64091.14.peg.1715; -.
DR   HOGENOM; CLU_032621_0_1_2; -.
DR   InParanoid; Q9HN66; -.
DR   OMA; YPEIWSD; -.
DR   OrthoDB; 33997at2157; -.
DR   PhylomeDB; Q9HN66; -.
DR   Proteomes; UP000000554; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004830; F:tryptophan-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0006436; P:tryptophanyl-tRNA aminoacylation; IBA:GO_Central.
DR   CDD; cd00806; TrpRS_core; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_00140_A; Trp_tRNA_synth_A; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002305; aa-tRNA-synth_Ic.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR002306; Trp-tRNA-ligase.
DR   InterPro; IPR020653; Tryptophan-tRNA-ligase_arc.
DR   Pfam; PF00579; tRNA-synt_1b; 1.
DR   PRINTS; PR01039; TRNASYNTHTRP.
DR   TIGRFAMs; TIGR00233; trpS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..380
FT                   /note="Tryptophan--tRNA ligase 2"
FT                   /id="PRO_0000136721"
FT   MOTIF           74..82
FT                   /note="'HIGH' region"
FT   MOTIF           249..253
FT                   /note="'KMSKS' region"
SQ   SEQUENCE   380 AA;  41937 MW;  0F7B2B955386404F CRC64;
     MTADGNDVTP YAVESDDLDY EKLLARFGAD ELTDDQRARF PDHPLVNRGL FYAGRDVDDF
     LTAGEQSIVT GVGPSGPMHL GHAMVFYFAR RLQDEFGARV YVPLSDDEKY WFKDQTPAET
     GDYLRANLRD LLAVGFDPEL TRIVVDTRDA DVLYPLATAF AGDVRHATLQ NVYGEPDNVG
     QAFYPAVQTA HLLLPQLVHG EHETLVPIAV DQDPHVRVSR DVAAKARYPV GKPGALLMQF
     LPSLAGPGKM SSSAGVSIRL TDSPDTVREK VRTHAYTGGR ASVEEHRAKG GVPAEDVPFQ
     YLSAFFEPDD AELARIEREY RAGDLLSGEL KDLAADRITE FLAAHQRRRA ALGDVTEALD
     AFRLTDDERQ RARDAVGFGY