ID   SYWC_DICDI              Reviewed;         400 AA.
AC   Q55DZ8;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Tryptophan--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.2;
DE   AltName: Full=Tryptophanyl-tRNA synthetase;
DE            Short=TrpRS;
GN   Name=trpS; ORFNames=DDB_G0269454;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-
CC         tryptophanyl-tRNA(Trp); Xref=Rhea:RHEA:24080, Rhea:RHEA-COMP:9671,
CC         Rhea:RHEA-COMP:9705, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57912, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78535, ChEBI:CHEBI:456215; EC=6.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000005; EAL72077.1; -; Genomic_DNA.
DR   RefSeq; XP_645983.1; XM_640891.1.
DR   AlphaFoldDB; Q55DZ8; -.
DR   SMR; Q55DZ8; -.
DR   STRING; 44689.DDB0231245; -.
DR   PaxDb; Q55DZ8; -.
DR   EnsemblProtists; EAL72077; EAL72077; DDB_G0269454.
DR   GeneID; 8616928; -.
DR   KEGG; ddi:DDB_G0269454; -.
DR   dictyBase; DDB_G0269454; trpS.
DR   eggNOG; KOG2145; Eukaryota.
DR   HOGENOM; CLU_032621_0_1_1; -.
DR   InParanoid; Q55DZ8; -.
DR   OMA; SIYHRFM; -.
DR   PhylomeDB; Q55DZ8; -.
DR   PRO; PR:Q55DZ8; -.
DR   Proteomes; UP000002195; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004830; F:tryptophan-tRNA ligase activity; ISS:dictyBase.
DR   GO; GO:0006436; P:tryptophanyl-tRNA aminoacylation; IBA:GO_Central.
DR   CDD; cd00806; TrpRS_core; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR002305; aa-tRNA-synth_Ic.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR002306; Trp-tRNA-ligase.
DR   Pfam; PF00579; tRNA-synt_1b; 1.
DR   PRINTS; PR01039; TRNASYNTHTRP.
DR   TIGRFAMs; TIGR00233; trpS; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..400
FT                   /note="Tryptophan--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000327792"
FT   MOTIF           95..104
FT                   /note="'HIGH' region"
FT   MOTIF           281..285
FT                   /note="'KMSKS' region"
SQ   SEQUENCE   400 AA;  45850 MW;  38FC775E37AE342C CRC64;
     MTETTPTTNE TKEQVITPWE VEAAPGGSVD YMKLVDQFGS TVISEELIAR FEKVTGKRAH
     HFLRRGIFFS HRDLKEILDH HESGKKWFLY TGRGPSSGSL HFGHLLPFTF TKYLQDAFNV
     PLVVQMTNDE KFLWKDMTLE ESIKFTHNNV KDIIALGFDI QKTFIFSNLE YIHHLYPNVL
     KIARCVNLNQ IQNIFGFKES DAIGKFTFPP VQAAPCFPDS FPHIFPLNDP EIKNIRCLIP
     CAIDQDPYFR MTRDIAHRIG HQKPALIHSK FFPALQGHNT KMSASDTNSA VYLSDTPDQV
     KDKIKKHAFS GGGATKEEQE KNGADLSVDI TYEYLTFMLE DDEQLKDIAH RYSTGKMMTG
     EIKQILIDLM NKIIIRHKEA RAKITDEVLS TFMSIRKLNF