ID   SYW_RALSO               Reviewed;         400 AA.
AC   Q8Y0A1;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Tryptophan--tRNA ligase;
DE            EC=6.1.1.2;
DE   AltName: Full=Tryptophanyl-tRNA synthetase;
DE            Short=TrpRS;
GN   Name=trpS; OrderedLocusNames=RSc1143; ORFNames=RS04610;
OS   Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=267608;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GMI1000;
RX   PubMed=11823852; DOI=10.1038/415497a;
RA   Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA   Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA   Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA   Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA   Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT   "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL   Nature 415:497-502(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-
CC         tryptophanyl-tRNA(Trp); Xref=Rhea:RHEA:24080, Rhea:RHEA-COMP:9671,
CC         Rhea:RHEA-COMP:9705, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57912, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78535, ChEBI:CHEBI:456215; EC=6.1.1.2;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; AL646052; CAD14845.1; -; Genomic_DNA.
DR   RefSeq; WP_011001093.1; NC_003295.1.
DR   AlphaFoldDB; Q8Y0A1; -.
DR   SMR; Q8Y0A1; -.
DR   STRING; 267608.RSc1143; -.
DR   EnsemblBacteria; CAD14845; CAD14845; RSc1143.
DR   GeneID; 60500656; -.
DR   KEGG; rso:RSc1143; -.
DR   PATRIC; fig|267608.8.peg.1161; -.
DR   eggNOG; COG0180; Bacteria.
DR   HOGENOM; CLU_029244_0_1_4; -.
DR   OMA; GWGQFKP; -.
DR   Proteomes; UP000001436; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004830; F:tryptophan-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006436; P:tryptophanyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd00806; TrpRS_core; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002305; aa-tRNA-synth_Ic.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR002306; Trp-tRNA-ligase.
DR   Pfam; PF00579; tRNA-synt_1b; 2.
DR   PRINTS; PR01039; TRNASYNTHTRP.
DR   TIGRFAMs; TIGR00233; trpS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..400
FT                   /note="Tryptophan--tRNA ligase"
FT                   /id="PRO_0000136665"
FT   REGION          173..241
FT                   /note="Insert"
FT   REGION          280..305
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           12..20
FT                   /note="'HIGH' region"
FT   MOTIF           265..269
FT                   /note="'KMSKS' region"
FT   COMPBIAS        280..303
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         268
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   400 AA;  45484 MW;  D551F6CE02657DAA CRC64;
     MFPERVLSGM RPTGALHLGH YHGVLKNWVR LQAEYPCFFF VADWHALTTH YESPEVIEES
     VWEMLIDWLA AGVDPTQATL FIQSRVPEHA ELFLLLSMGT PLGWLERVPT YKDQIEKLKE
     KDLSTYGFLG YPLLQAADIL IYRAGFVPVG EDQVPHVEMT REVARRFNYL YGREPGFEQK
     ALDAAKKLGG KRAKLYLELR TAHQERGEDD ALEQARALLA ESQSLSMGDR ERLFGYLEGA
     RKIILPEPQV LLTEASRMPG LDGQKMSKSY GNTIRMREDK ASVEKKVRTM PTDPARVRRT
     DPGDPGKCPV WQLHQVYSDA DTREWVQKGC RSAGIGCLEC KQPVIDGILR EQQPMLERAQ
     KYMDDPSLLR AIIADGCDTA HKVTQETMRE VREAMGLTYS