ID   SYW_SULTO               Reviewed;         381 AA.
AC   Q976M1; F9VMM5;
DT   16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2002, sequence version 2.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Tryptophan--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00140};
DE            EC=6.1.1.2 {ECO:0000255|HAMAP-Rule:MF_00140};
DE   AltName: Full=Tryptophanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00140};
DE            Short=TrpRS {ECO:0000255|HAMAP-Rule:MF_00140};
GN   Name=trpS {ECO:0000255|HAMAP-Rule:MF_00140}; OrderedLocusNames=STK_01690;
OS   Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
OS   (Sulfolobus tokodaii).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Sulfurisphaera.
OX   NCBI_TaxID=273063;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX   PubMed=11572479; DOI=10.1093/dnares/8.4.123;
RA   Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M.,
RA   Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y.,
RA   Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T.,
RA   Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K.,
RA   Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H.;
RT   "Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon,
RT   Sulfolobus tokodaii strain7.";
RL   DNA Res. 8:123-140(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-
CC         tryptophanyl-tRNA(Trp); Xref=Rhea:RHEA:24080, Rhea:RHEA-COMP:9671,
CC         Rhea:RHEA-COMP:9705, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57912, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78535, ChEBI:CHEBI:456215; EC=6.1.1.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00140};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00140}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00140}.
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DR   EMBL; BA000023; BAK54171.1; -; Genomic_DNA.
DR   RefSeq; WP_010978108.1; NC_003106.2.
DR   AlphaFoldDB; Q976M1; -.
DR   SMR; Q976M1; -.
DR   STRING; 273063.STK_01690; -.
DR   EnsemblBacteria; BAK54171; BAK54171; STK_01690.
DR   GeneID; 42799641; -.
DR   KEGG; sto:STK_01690; -.
DR   PATRIC; fig|273063.9.peg.208; -.
DR   eggNOG; arCOG01887; Archaea.
DR   OMA; SIYHRFM; -.
DR   OrthoDB; 33997at2157; -.
DR   Proteomes; UP000001015; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004830; F:tryptophan-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006436; P:tryptophanyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd00806; TrpRS_core; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_00140_A; Trp_tRNA_synth_A; 1.
DR   InterPro; IPR002305; aa-tRNA-synth_Ic.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR002306; Trp-tRNA-ligase.
DR   InterPro; IPR020653; Tryptophan-tRNA-ligase_arc.
DR   Pfam; PF00579; tRNA-synt_1b; 1.
DR   PRINTS; PR01039; TRNASYNTHTRP.
DR   TIGRFAMs; TIGR00233; trpS; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..381
FT                   /note="Tryptophan--tRNA ligase"
FT                   /id="PRO_0000136734"
FT   MOTIF           82..90
FT                   /note="'HIGH' region"
FT   MOTIF           254..258
FT                   /note="'KMSKS' region"
SQ   SEQUENCE   381 AA;  44718 MW;  109F5A56AF7D7159 CRC64;
     MAQDFNVTPW EVKGKVDYDK LIVQFGTQKI TSELKEKIKS IINDELHVML RRDVFFSHRD
     LDLVLKDYQD GKGFFLYTGR APSLGMHIGH LIPFIFTKWL QDKFNVNLYI EITDDEKFMR
     NPEYTLDQTR QWAYDNILDI IAVGFNPDKT FIFQDTEYIR NMYPIAIKIA KKLTFSEVRA
     TFGLDTSSNI GIIWYPALQI APTMFEKRRC LIPAGIDQDP YWRLQRDIAE SLGYYKAAQI
     HSKFLPPLTG PEGKMSSSQP ETAIYLTDDP KTVERKIMKY AFSGGQPTIE LHRKYGGNPD
     IDVSFQWLYM FFEPDDNKIK KIEEDYRSGA LLTGELKQIL IEKLNDFLEE HRQKREEAKK
     LVNVFKYDGE LAREMWRKIH E