BOS1_YEAST
ID BOS1_YEAST Reviewed; 244 AA.
AC P25385; D6VY78;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Protein transport protein BOS1;
DE AltName: Full=Bet one suppressor 1;
GN Name=BOS1; OrderedLocusNames=YLR078C; ORFNames=L9449.9;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2007627; DOI=10.1083/jcb.113.1.55;
RA Shim J., Newman A.P., Ferro-Novick S.;
RT "The BOS1 gene encodes an essential 27-kD putative membrane protein that is
RT required for vesicular transport from the ER to the Golgi complex in
RT yeast.";
RL J. Cell Biol. 113:55-64(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, AND INTERACTION WITH BET1; SEC22 AND SED5.
RX PubMed=11001058; DOI=10.1038/35025076;
RA Parlati F., McNew J.A., Fukuda R., Miller R., Sollner T.H., Rothman J.E.;
RT "Topological restriction of SNARE-dependent membrane fusion.";
RL Nature 407:194-198(2000).
RN [5]
RP INTERACTION WITH YIF1 AND YIP1.
RX PubMed=12657649; DOI=10.1074/jbc.m302406200;
RA Barrowman J., Wang W., Zhang Y., Ferro-Novick S.;
RT "The Yip1p.Yif1p complex is required for the fusion competence of
RT endoplasmic reticulum-derived vesicles.";
RL J. Biol. Chem. 278:19878-19884(2003).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: SNARE required for targeting and fusion of ER-derived
CC transport vesicles with the Golgi complex.
CC {ECO:0000269|PubMed:11001058}.
CC -!- SUBUNIT: Component of a SNARE complex consisting of SED5, BOS1, BET1
CC and SEC22 or YKT6. Interacts with YIF1 and YIP1.
CC {ECO:0000269|PubMed:11001058, ECO:0000269|PubMed:12657649}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type IV membrane protein {ECO:0000305}. Endoplasmic reticulum
CC membrane {ECO:0000305}; Single-pass type IV membrane protein
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the BOS1 family. {ECO:0000305}.
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DR EMBL; X57792; CAA40933.1; -; Genomic_DNA.
DR EMBL; Z73250; CAA97636.1; -; Genomic_DNA.
DR EMBL; U53880; AAB67582.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09394.1; -; Genomic_DNA.
DR PIR; A39206; A39206.
DR RefSeq; NP_013179.1; NM_001181965.1.
DR AlphaFoldDB; P25385; -.
DR SMR; P25385; -.
DR BioGRID; 31351; 686.
DR ComplexPortal; CPX-1854; Golgi SNARE complex SED5-BOS1-BET1-SEC22.
DR DIP; DIP-2020N; -.
DR IntAct; P25385; 10.
DR MINT; P25385; -.
DR STRING; 4932.YLR078C; -.
DR iPTMnet; P25385; -.
DR MaxQB; P25385; -.
DR PaxDb; P25385; -.
DR PRIDE; P25385; -.
DR EnsemblFungi; YLR078C_mRNA; YLR078C; YLR078C.
DR GeneID; 850767; -.
DR KEGG; sce:YLR078C; -.
DR SGD; S000004068; BOS1.
DR VEuPathDB; FungiDB:YLR078C; -.
DR eggNOG; KOG3251; Eukaryota.
DR HOGENOM; CLU_078260_1_0_1; -.
DR InParanoid; P25385; -.
DR OMA; FCWLVIH; -.
DR BioCyc; YEAST:G3O-32229-MON; -.
DR Reactome; R-SCE-204005; COPII-mediated vesicle transport.
DR Reactome; R-SCE-5694530; Cargo concentration in the ER.
DR Reactome; R-SCE-6807878; COPI-mediated anterograde transport.
DR PRO; PR:P25385; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; P25385; protein.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:SGD.
DR GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IDA:SGD.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:1990674; C:Golgi cis cisterna membrane; IC:ComplexPortal.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IDA:SGD.
DR GO; GO:0031902; C:late endosome membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IDA:ComplexPortal.
DR GO; GO:0031201; C:SNARE complex; IDA:SGD.
DR GO; GO:0005484; F:SNAP receptor activity; IDA:SGD.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IMP:SGD.
DR GO; GO:0006886; P:intracellular protein transport; IDA:ComplexPortal.
DR GO; GO:0006906; P:vesicle fusion; IDA:SGD.
DR GO; GO:0048280; P:vesicle fusion with Golgi apparatus; IDA:ComplexPortal.
DR InterPro; IPR027027; GOSR2/Membrin/Bos1.
DR PIRSF; PIRSF028865; Membrin-2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus;
KW Membrane; Protein transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..244
FT /note="Protein transport protein BOS1"
FT /id="PRO_0000207557"
FT TOPO_DOM 1..222
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..240
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..244
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
SQ SEQUENCE 244 AA; 27500 MW; E2F13D444ADC0D93 CRC64;
MNALYNHAVK QKNQLQQELA RFEKNSVTAP ISLQGSISAT LVSLEKTVKQ YAEHLNRYKE
DTNAEEIDPK FANRLATLTQ DLHDFTAKFK DLKQSYNENN SRTQLFGSGA SHVMDSDNPF
STSETIMNKR NVGGASANGK EGSSNGGGLP LYQGLQKEQS VFERGNAQLD YILEMGQQSF
ENIVEQNKIL SKVQDRMSNG LRTLGVSEQT ITSINKRVFK DKLVFWIALI LLIIGIYYVL
KWLR
