BOSS_DROME
ID BOSS_DROME Reviewed; 896 AA.
AC P22815; Q9VBJ5;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 3.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Protein bride of sevenless;
DE Flags: Precursor;
GN Name=boss; ORFNames=CG8285;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2276620; DOI=10.1101/gad.4.11.1835;
RA Hart A.C., Kraemer H., van Vactor D.L. Jr., Paidhungat M., Zipursky S.L.;
RT "Induction of cell fate in the Drosophila retina: the bride of sevenless
RT protein is predicted to contain a large extracellular domain and seven
RT transmembrane segments.";
RL Genes Dev. 4:1835-1847(1990).
RN [2]
RP SEQUENCE REVISION.
RA Kraemer H.;
RL Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP FUNCTION.
RX PubMed=1857416; DOI=10.1038/352207a0;
RA Kraemer H., Cagan R.L., Zipursky S.L.;
RT "Interaction of bride of sevenless membrane-bound ligand and the sevenless
RT tyrosine-kinase receptor.";
RL Nature 352:207-212(1991).
CC -!- FUNCTION: Acts as a ligand for sevenless tyrosine-kinase receptor
CC during eye development. {ECO:0000269|PubMed:1857416}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed exclusively by R8 photoreceptor cells and
CC is internalized in a sev-dependent manner by R7 cells.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 3 family.
CC {ECO:0000305}.
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DR EMBL; X55887; CAA39373.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56539.1; -; Genomic_DNA.
DR RefSeq; NP_542440.1; NM_080709.3.
DR AlphaFoldDB; P22815; -.
DR BioGRID; 68046; 6.
DR IntAct; P22815; 1.
DR STRING; 7227.FBpp0084323; -.
DR GlyGen; P22815; 4 sites.
DR PaxDb; P22815; -.
DR PRIDE; P22815; -.
DR DNASU; 43146; -.
DR EnsemblMetazoa; FBtr0084949; FBpp0084323; FBgn0000206.
DR GeneID; 43146; -.
DR KEGG; dme:Dmel_CG8285; -.
DR CTD; 43146; -.
DR FlyBase; FBgn0000206; boss.
DR VEuPathDB; VectorBase:FBgn0000206; -.
DR eggNOG; KOG1056; Eukaryota.
DR HOGENOM; CLU_010513_1_0_1; -.
DR InParanoid; P22815; -.
DR OMA; LQQCTQY; -.
DR OrthoDB; 671877at2759; -.
DR PhylomeDB; P22815; -.
DR Reactome; R-DME-418594; G alpha (i) signalling events.
DR Reactome; R-DME-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR SignaLink; P22815; -.
DR BioGRID-ORCS; 43146; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 43146; -.
DR PRO; PR:P22815; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0000206; Expressed in head capsule and 7 other tissues.
DR ExpressionAtlas; P22815; baseline and differential.
DR Genevisible; P22815; DM.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:FlyBase.
DR GO; GO:0098839; C:postsynaptic density membrane; IBA:GO_Central.
DR GO; GO:0035997; C:rhabdomere microvillus membrane; IDA:FlyBase.
DR GO; GO:0001640; F:adenylate cyclase inhibiting G protein-coupled glutamate receptor activity; IBA:GO_Central.
DR GO; GO:0099530; F:G protein-coupled receptor activity involved in regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0099583; F:neurotransmitter receptor activity involved in regulation of postsynaptic cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:0048018; F:receptor ligand activity; IDA:FlyBase.
DR GO; GO:0005118; F:sevenless binding; IDA:FlyBase.
DR GO; GO:0007216; P:G protein-coupled glutamate receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0060250; P:germ-line stem-cell niche homeostasis; IMP:FlyBase.
DR GO; GO:0042593; P:glucose homeostasis; IMP:FlyBase.
DR GO; GO:0055088; P:lipid homeostasis; IMP:FlyBase.
DR GO; GO:0007465; P:R7 cell fate commitment; IMP:FlyBase.
DR GO; GO:0045470; P:R8 cell-mediated photoreceptor organization; IMP:FlyBase.
DR GO; GO:0051966; P:regulation of synaptic transmission, glutamatergic; IBA:GO_Central.
DR GO; GO:0009749; P:response to glucose; IMP:FlyBase.
DR GO; GO:0045500; P:sevenless signaling pathway; IDA:FlyBase.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR002956; Bride_of_7less.
DR InterPro; IPR017978; GPCR_3_C.
DR Pfam; PF00003; 7tm_3; 1.
DR PRINTS; PR01223; BRIDEOF7LESS.
PE 2: Evidence at transcript level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Sensory transduction; Signal; Transducer;
KW Transmembrane; Transmembrane helix; Vision.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..896
FT /note="Protein bride of sevenless"
FT /id="PRO_0000012971"
FT TOPO_DOM 32..530
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 531..554
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 570..588
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 615..637
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 655..676
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 693..712
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 728..748
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 759..781
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 782..896
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 38..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 825..844
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 861..896
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..84
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 307
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 474
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 485
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 896 AA; 99950 MW; 580AEBC0FA208E24 CRC64;
MKVMDALQSG RRKPLPVALL CILVTVFCVL ECHGADLTSP TKKSAPLRIT KPQPTSQQAK
PISITTRAPT TVASTTDDEV SSSVDGQLAP LISSTTEGPS SGTTASLVPE ICLNGLQLTV
NSADEGTVIR KQEEFVKILE GDVVLSVLTK DPDSALFVIN RVNQANLIMA DFEIGIRAIS
IDNASLAENL LIQEVQFLQQ CTTYSMGIFV DWELYKQLES VIKDLEYNIW PIPGTRAHLF
PKVAHLLHQM PWGEKIASVE IATETLEMYN EFMEAARQEH MCLMHFKSDD NVYIMFGNKL
ASHFKENGTL FSVPTDRTDD EFLADLPNRA FVLMENEIDL STAVELDATP TALDEILIGK
SVLPSRVLSF AGSIIDLMNW LRGSLSKHCK RGEEHDLYVL ESCFNFLNFI EDWRTSEYRQ
AHDTAEILSL LLMRKLGTAM NFQMYQKKVL TLDKITGESR TELREIASQN FVTNVTTYYH
YNRDNHTSLE LKTKFGQVFN CQYSAGDNRR YPFLFDGESV MFWRIKMDTW VATGLTAAIL
GLIATLAILV FIVVRISLGD VFEGNPTTSI LLLLSLILVF CSFVPYSIEY VGEQRNSHVT
FEDAQTLNTL CAVRVFIMTL VYCFVFSLLL CRAVMLASIG SEGGFLSHVN GYIQAVICAF
SVVAQVGMSV QLLVVMHVAS ETVSCENIYY GRWLWGLLAY DFALLCCVGA LIPSIYRSQR
NYREGILIVI GSVLIMVIWV AWIALSLFGD EWRDAAIPLG LQASGWAVLV GILIPRTFLI
VRGIERSDIA QALPSLTSLA FAQNNQYSSE QSVYECVNPA MRHCSQDEVN HQSPSEIPTL
PLRGGGPRRQ QFFANLRQAN ANINPQRPPP RPQQSPSRSS VSSLPPSPDH NKITRF
