BOULE_DROME
ID BOULE_DROME Reviewed; 228 AA.
AC Q24207; Q8T472; Q9VSU4; Q9VSU5;
DT 01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Protein boule;
GN Name=bol; ORFNames=CG4760;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=8657280; DOI=10.1038/381783a0;
RA Eberhart C.G., Maines J.Z., Wasserman S.A.;
RT "Meiotic cell cycle requirement for a fly homologue of human Deleted in
RT Azoospermia.";
RL Nature 381:783-785(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 43-228 (ISOFORM 1), AND
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 167-228 (ISOFORM 2).
RC STRAIN=Berkeley; TISSUE=Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=9882490; DOI=10.1006/dbio.1998.9098;
RA Cheng M.H., Maines J.Z., Wasserman S.A.;
RT "Biphasic subcellular localization of the DAZL-related protein boule in
RT Drosophila spermatogenesis.";
RL Dev. Biol. 204:567-576(1998).
RN [6]
RP FUNCTION.
RX PubMed=10559904; DOI=10.1038/11091;
RA Maines J.Z., Wasserman S.A.;
RT "Post-transcriptional regulation of the meiotic Cdc25 protein Twine by the
RT Dazl orthologue Boule.";
RL Nat. Cell Biol. 1:171-174(1999).
RN [7]
RP FUNCTION.
RX PubMed=12499397; DOI=10.1093/hmg/ddg017;
RA Xu E.Y., Lee D.F., Klebes A., Turek P.J., Kornberg T.B., Reijo Pera R.A.;
RT "Human BOULE gene rescues meiotic defects in infertile flies.";
RL Hum. Mol. Genet. 12:169-175(2003).
RN [8]
RP INTERACTION WITH ORB2.
RX PubMed=23209437; DOI=10.1371/journal.pgen.1003079;
RA Xu S., Hafer N., Agunwamba B., Schedl P.;
RT "The CPEB protein Orb2 has multiple functions during spermatogenesis in
RT Drosophila melanogaster.";
RL PLoS Genet. 8:E1003079-E1003079(2012).
CC -!- FUNCTION: RNA-binding protein that plays a central role in
CC spermatogenesis. Required for meiotic entry and germline
CC differentiation, at the transition between G2 and M phases of meiosis
CC I. Acts by regulating translation of specific mRNAs, possibly by
CC binding to their 3'-UTR. Essential for translation of twine (twe) mRNA.
CC Required for the expression of various genes such as CG6784, CG17210,
CC CG15841 scpr-B, scpr-C, and rho-6. {ECO:0000269|PubMed:10559904,
CC ECO:0000269|PubMed:12499397, ECO:0000269|PubMed:8657280}.
CC -!- SUBUNIT: Interacts with the translational regulator orb2.
CC {ECO:0000269|PubMed:23209437}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9882490}. Cytoplasm
CC {ECO:0000269|PubMed:9882490}. Note=Nuclear in primary spermatocytes
CC until near the end of the meiotic prophase and cytoplasmic localization
CC from then onward.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q24207-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q24207-2; Sequence=VSP_009450, VSP_009451;
CC -!- TISSUE SPECIFICITY: Testis specific. {ECO:0000269|PubMed:8657280}.
CC -!- DEVELOPMENTAL STAGE: Not expressed in embryos. Expressed in larvae,
CC pupae and adult males. Expressed during spermatogenesis.
CC {ECO:0000269|PubMed:8657280}.
CC -!- SIMILARITY: Belongs to the RRM DAZ family. {ECO:0000255|PROSITE-
CC ProRule:PRU01238}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL90061.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
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DR EMBL; U51858; AAC47133.1; -; mRNA.
DR EMBL; AE014296; AAF50316.3; -; Genomic_DNA.
DR EMBL; AE014296; AAF50317.3; -; Genomic_DNA.
DR EMBL; AI063811; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AY089323; AAL90061.1; ALT_SEQ; mRNA.
DR PIR; S68988; S68988.
DR RefSeq; NP_001286992.1; NM_001300063.1. [Q24207-1]
DR RefSeq; NP_523989.1; NM_079265.4. [Q24207-1]
DR RefSeq; NP_729456.1; NM_168318.3. [Q24207-1]
DR RefSeq; NP_729457.1; NM_168319.2.
DR RefSeq; NP_729458.1; NM_168320.2.
DR AlphaFoldDB; Q24207; -.
DR SMR; Q24207; -.
DR BioGRID; 64449; 11.
DR DIP; DIP-20744N; -.
DR IntAct; Q24207; 5.
DR STRING; 7227.FBpp0304242; -.
DR PaxDb; Q24207; -.
DR DNASU; 39049; -.
DR EnsemblMetazoa; FBtr0076538; FBpp0076265; FBgn0011206. [Q24207-1]
DR EnsemblMetazoa; FBtr0076540; FBpp0076267; FBgn0011206. [Q24207-1]
DR EnsemblMetazoa; FBtr0344979; FBpp0311234; FBgn0011206. [Q24207-1]
DR GeneID; 39049; -.
DR KEGG; dme:Dmel_CG4760; -.
DR UCSC; CG4760-RA; d. melanogaster. [Q24207-1]
DR CTD; 39049; -.
DR FlyBase; FBgn0011206; bol.
DR VEuPathDB; VectorBase:FBgn0011206; -.
DR eggNOG; KOG0118; Eukaryota.
DR GeneTree; ENSGT00530000063480; -.
DR InParanoid; Q24207; -.
DR OMA; PAINNIP; -.
DR PhylomeDB; Q24207; -.
DR BioGRID-ORCS; 39049; 0 hits in 3 CRISPR screens.
DR ChiTaRS; bol; fly.
DR GenomeRNAi; 39049; -.
DR PRO; PR:Q24207; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0011206; Expressed in testis and 28 other tissues.
DR ExpressionAtlas; Q24207; baseline and differential.
DR Genevisible; Q24207; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0043204; C:perikaryon; IDA:FlyBase.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; ISS:FlyBase.
DR GO; GO:0003723; F:RNA binding; IMP:UniProtKB.
DR GO; GO:0008494; F:translation activator activity; IBA:GO_Central.
DR GO; GO:0070935; P:3'-UTR-mediated mRNA stabilization; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0008315; P:G2/MI transition of meiotic cell cycle; IMP:UniProtKB.
DR GO; GO:0045948; P:positive regulation of translational initiation; IBA:GO_Central.
DR GO; GO:0045924; P:regulation of female receptivity; IMP:UniProtKB.
DR GO; GO:0006417; P:regulation of translation; IGI:FlyBase.
DR GO; GO:0048137; P:spermatocyte division; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; TAS:FlyBase.
DR CDD; cd12412; RRM_DAZL_BOULE; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR037366; BOULE/DAZ.
DR InterPro; IPR034988; DAZ_BOULE_RRM.
DR InterPro; IPR043628; DAZ_dom.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR PANTHER; PTHR11176; PTHR11176; 1.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS51890; DAZ; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Cytoplasm; Developmental protein;
KW Differentiation; Nucleus; Reference proteome; RNA-binding; Spermatogenesis;
KW Translation regulation.
FT CHAIN 1..228
FT /note="Protein boule"
FT /id="PRO_0000081497"
FT DOMAIN 33..110
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 151..178
FT /note="DAZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01238"
FT REGION 193..228
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 193..215
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 189
FT /note="E -> G (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_009450"
FT VAR_SEQ 190
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_009451"
SQ SEQUENCE 228 AA; 24682 MW; 2A5EA1C3223985C3 CRC64;
MHKIAAAPPP SATPGGGLET PLAAPKYGTL IPNRIFVGGI SGDTTEADLT RVFSAYGTVK
STKIIVDRAG VSKGYGFVTF ETEQEAQRLQ ADGECVVLRD RKLNIAPAIK KQPNPLQSIV
ATNGAVYYTT TPPAPISNIP MDQFAAAVYP PAAGVPAIYP PSAMQYQPFY QYYSVPMNVP
TIWPQNYQEN HSPLLHSPTS NPHSPHSQSH PQSPCWSIED LRDTLPRV
