BOWL_XENLA
ID BOWL_XENLA Reviewed; 153 AA.
AC Q25QX6; A8QWV4;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=Protein ripply2.2;
DE AltName: Full=Protein bowline;
GN Name=ripply2.2; Synonyms=bowline, ripply2;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAE86929.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH TLE4, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, DOMAIN, AND MUTAGENESIS OF 58-TRP--TRP-61.
RC TISSUE=Mesoderm {ECO:0000269|PubMed:16586348};
RX PubMed=16586348; DOI=10.1387/ijdb.052138ak;
RA Kondow A., Hitachi K., Ikegame T., Asashima M.;
RT "Bowline, a novel protein localized to the presomitic mesoderm, interacts
RT with Groucho/TLE in Xenopus.";
RL Int. J. Dev. Biol. 50:473-479(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=18035347; DOI=10.1016/j.ydbio.2007.10.015;
RA Hitachi K., Kondow A., Danno H., Inui M., Uchiyama H., Asashima M.;
RT "Tbx6, Thylacine1, and E47 synergistically activate bowline expression in
RT Xenopus somitogenesis.";
RL Dev. Biol. 313:816-828(2008).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Gastrula;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, INTERACTION WITH TBX6 AND TLE4, DOMAIN, AND MUTAGENESIS OF ARG-59
RP AND 58-TRP--TRP-61.
RX PubMed=17577580; DOI=10.1016/j.bbrc.2007.05.211;
RA Kondow A., Hitachi K., Okabayashi K., Hayashi N., Asashima M.;
RT "Bowline mediates association of the transcriptional corepressor XGrg-4
RT with Tbx6 during somitogenesis in Xenopus.";
RL Biochem. Biophys. Res. Commun. 359:959-964(2007).
RN [5]
RP INDUCTION.
RX PubMed=18510946; DOI=10.1016/j.bbrc.2008.05.083;
RA Hitachi K., Danno H., Kondow A., Ohnuma K., Uchiyama H., Ishiura S.,
RA Kurisaki A., Asashima M.;
RT "Physical interaction between Tbx6 and mespb is indispensable for the
RT activation of bowline expression during Xenopus somitogenesis.";
RL Biochem. Biophys. Res. Commun. 372:607-612(2008).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=19247927; DOI=10.1387/ijdb.082823kh;
RA Hitachi K., Danno H., Tazumi S., Aihara Y., Uchiyama H., Okabayashi K.,
RA Kondow A., Asashima M.;
RT "The Xenopus Bowline/Ripply family proteins negatively regulate the
RT transcriptional activity of T-box transcription factors.";
RL Int. J. Dev. Biol. 53:631-639(2009).
CC -!- FUNCTION: Required during somitogenesis for the formation of somite
CC boundaries. Represses the expression of genes involved in somite
CC segmentation by acting with the corepressor tle4 to down-regulate the
CC transcriptional activity of tbx6. May act by regulating the activity of
CC tle4. Represses transcription of delta2, thy1 and ripply2.2/bowline
CC itself. {ECO:0000269|PubMed:16586348, ECO:0000269|PubMed:17577580,
CC ECO:0000269|PubMed:19247927}.
CC -!- SUBUNIT: Interacts with tle4 and tbx6, and mediates interaction between
CC these proteins. {ECO:0000269|PubMed:16586348,
CC ECO:0000269|PubMed:17577580}.
CC -!- INTERACTION:
CC Q25QX6; O42478: tle4; NbExp=2; IntAct=EBI-2946647, EBI-2946644;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16586348}.
CC -!- TISSUE SPECIFICITY: Expressed in the presomitic mesoderm (PSM) in the
CC anterior halves of somitomeres S-I, S-II and S-III.
CC {ECO:0000269|PubMed:16586348, ECO:0000269|PubMed:18035347,
CC ECO:0000269|PubMed:19247927}.
CC -!- INDUCTION: Part of a negative feedback loop during somitogenesis.
CC Expression is activated by tbx6 together with thy1 and tcf3.
CC {ECO:0000269|PubMed:18035347, ECO:0000269|PubMed:18510946,
CC ECO:0000269|PubMed:19247927}.
CC -!- DOMAIN: The ripply homology domain is required for transcriptional
CC repression.
CC -!- DOMAIN: The WRPW motif is required for binding to tle/groucho proteins
CC and transcriptional repression. {ECO:0000269|PubMed:16586348,
CC ECO:0000269|PubMed:17577580}.
CC -!- SIMILARITY: Belongs to the ripply family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB105905; BAE86929.1; -; mRNA.
DR EMBL; AB274961; BAF91354.1; -; Genomic_DNA.
DR EMBL; BC169341; AAI69341.1; -; mRNA.
DR EMBL; BC169343; AAI69343.1; -; mRNA.
DR RefSeq; NP_001089164.1; NM_001095695.1.
DR AlphaFoldDB; Q25QX6; -.
DR IntAct; Q25QX6; 1.
DR GeneID; 734201; -.
DR KEGG; xla:734201; -.
DR CTD; 734201; -.
DR Xenbase; XB-GENE-6251689; ripply2.2.S.
DR OrthoDB; 1613448at2759; -.
DR Proteomes; UP000186698; Chromosome 5S.
DR Bgee; 734201; Expressed in neurula embryo and 1 other tissue.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0017053; C:transcription repressor complex; IPI:UniProtKB.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0001756; P:somitogenesis; IMP:UniProtKB.
DR InterPro; IPR028127; Ripply_fam.
DR PANTHER; PTHR16770; PTHR16770; 1.
DR Pfam; PF14998; Ripply; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..153
FT /note="Protein ripply2.2"
FT /id="PRO_0000307765"
FT REGION 93..128
FT /note="Ripply homology domain"
FT /evidence="ECO:0000269|PubMed:17577580"
FT REGION 127..153
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 58..61
FT /note="WRPW motif; required for transcriptional repression
FT and interaction with tle4"
FT /evidence="ECO:0000269|PubMed:16586348,
FT ECO:0000269|PubMed:17577580"
FT COMPBIAS 132..153
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 58..61
FT /note="WRPW->GGGG: Disrupts transcriptional repression and
FT interaction with tle4."
FT /evidence="ECO:0000269|PubMed:16586348,
FT ECO:0000269|PubMed:17577580"
FT MUTAGEN 58..61
FT /note="Missing: Disrupts transcriptional repression."
FT /evidence="ECO:0000269|PubMed:16586348,
FT ECO:0000269|PubMed:17577580"
FT MUTAGEN 59
FT /note="R->E: Disrupts transcriptional repression."
FT /evidence="ECO:0000269|PubMed:17577580"
SQ SEQUENCE 153 AA; 17754 MW; 6D593CA9C00C3107 CRC64;
MDNINTTIDI CGAASGLPSP GDSNSKRLRH RKYLPQRWKC EPRGPARTET QRPQTLFWRP
WLLKSHKPKT QAHPYARGLR ENPQEQKPVE YNHPVRLFWP KSKLLDNTYQ EAADLLRNFP
VQATISLYND SESDTDNEED SSEEEQDSGF ESE
