BOYB_DROME
ID BOYB_DROME Reviewed; 1059 AA.
AC Q9VNP5; Q7YTZ8;
DT 22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Putative ATP-dependent RNA helicase BoYb;
DE EC=3.6.4.13;
DE AltName: Full=Brother of Yb;
GN Name=BoYb; ORFNames=CG11133;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21863019; DOI=10.1038/emboj.2011.308;
RA Handler D., Olivieri D., Novatchkova M., Gruber F.S., Meixner K.,
RA Mechtler K., Stark A., Sachidanandam R., Brennecke J.;
RT "A systematic analysis of Drosophila TUDOR domain-containing proteins
RT identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway
RT factors.";
RL EMBO J. 30:3977-3993(2011).
CC -!- FUNCTION: Involved in primary piRNA biogenesis in germline cells.
CC {ECO:0000269|PubMed:21863019}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21863019}.
CC Note=Localizes to Yb bodies.
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DR EMBL; AE014296; AAF51884.1; -; Genomic_DNA.
DR EMBL; BT010084; AAQ22553.1; -; mRNA.
DR EMBL; BT058004; ACM16714.1; -; mRNA.
DR RefSeq; NP_649430.1; NM_141173.2.
DR AlphaFoldDB; Q9VNP5; -.
DR SMR; Q9VNP5; -.
DR BioGRID; 65740; 1.
DR IntAct; Q9VNP5; 4.
DR MINT; Q9VNP5; -.
DR STRING; 7227.FBpp0078210; -.
DR PaxDb; Q9VNP5; -.
DR PRIDE; Q9VNP5; -.
DR DNASU; 40512; -.
DR EnsemblMetazoa; FBtr0078559; FBpp0078210; FBgn0037205.
DR GeneID; 40512; -.
DR KEGG; dme:Dmel_CG11133; -.
DR UCSC; CG11133-RA; d. melanogaster.
DR CTD; 40512; -.
DR FlyBase; FBgn0037205; BoYb.
DR VEuPathDB; VectorBase:FBgn0037205; -.
DR eggNOG; KOG0334; Eukaryota.
DR GeneTree; ENSGT00940000167250; -.
DR HOGENOM; CLU_286417_0_0_1; -.
DR InParanoid; Q9VNP5; -.
DR OMA; EAPGLMR; -.
DR OrthoDB; 225652at2759; -.
DR PhylomeDB; Q9VNP5; -.
DR BioGRID-ORCS; 40512; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40512; -.
DR PRO; PR:Q9VNP5; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0037205; Expressed in ovary and 11 other tissues.
DR ExpressionAtlas; Q9VNP5; baseline and differential.
DR Genevisible; Q9VNP5; DM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0070725; C:Yb body; IDA:FlyBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0042078; P:germ-line stem cell division; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0010526; P:negative regulation of transposition, RNA-mediated; IMP:FlyBase.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cytoplasm; Helicase; Hydrolase; Meiosis; Nucleotide-binding;
KW Reference proteome; Repeat.
FT CHAIN 1..1059
FT /note="Putative ATP-dependent RNA helicase BoYb"
FT /id="PRO_0000425214"
FT DOMAIN 87..284
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 575..639
FT /note="Tudor"
FT REGION 756..787
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 54..82
FT /note="Q motif"
FT MOTIF 230..233
FT /note="DEAD box"
FT COMPBIAS 756..776
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 100..107
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 1045
FT /note="V -> A (in Ref. 3; AAQ22553)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1059 AA; 121400 MW; 3D1C1DAABB07C045 CRC64;
MIKISDEKTW QSSRDSVYEV GDSYSGQNAN RPLVATNCSE YAVAHANCQL RPVRRFAEVS
LLPDILETMR NLGLNRLLRL QSYTWPHLAG GSGHGAMIVG SPASGRTFAY IPPVCHAVSR
ALMDFTGQCE DVEHDISQPD RYGPIALILV PDLRRVHQVS AMCLALLRKA HKNDSVTLAL
NVSSTKSSQF FLKLLNGVGC LVATPAQLVW FWQEAPGLMR FPCLQFLVYD DVDLMSREQL
QDVQQVLQEI LPLSHSPQVV MVSKSYCHTL MSKLRAVNDK PALVFGDILE AALYGGTRIR
ISIMRSEAKA NAVVQMLQQC SPEEFRTVIF CSDDGDMQCL VAALEVQHYS CLPYYQTADL
EVRQQVHSWQ ARSNGVILLC TDNCPELDIR DAHTIIHHSM SHSWSKFKLR HLKISDNLCN
MVKPTASIVK KPLYSLVLLD DNNHRQLPRL VDFLQLHQKV DHRLVEVAKR IRQELGKARN
DQHQLCDQIL VLGKCYDPVC ESRHRLSHID RRPDYLPASG DVKVQLVKVY SPTHFCVRLL
EHLPPKGTWR MMEYSAVQEF RMQLTQIKEP RRYWPPVAGA ICMYHTTFTK ERVRVLKVAA
IKNTNIVQSD LTVKLQALDV DTRIFSTNCG KLFECPEALQ QEAPLACDLR LPGWVPYFGE
RSWTEENIRN VNLILTQLPK DHFLQAKILF VAAGTLFVQD LVAIMYADQF KAHVRHLSLA
RRLVEATLVK RSENAAEMIR EFFAEVIIED DIDENVQDSK EKANSKPHEK MKGKMTDQPA
KLQSQPPLSG RCLRLANMAH ESVKENQLHQ ELQERRYETP EIPHQSNESD IPQSNEDRFS
QLYECIMNCA SLQLEDESKP AKHPDHVLSE SVEFHKIMTN EDATPDHTQE KTALLLLPNN
VARPSVTYYQ TMTTLEFQVF LPEDDHDYKA LLLGAQLFFR AISKSSDLIL QFIMTLRFPY
SSMSHNIRGR TVYISVKKLL ALIDPLAFRE YRFLKPNHDL FDKVDKQLQE TQNRLVRFLE
DMNYVKRNFE GQEKRETSED EEVNVEGIER PDCHKIWDL
