BP09_BPT4
ID BP09_BPT4 Reviewed; 288 AA.
AC P10927;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 29-SEP-2021, entry version 114.
DE RecName: Full=Baseplate protein gp9 {ECO:0000305};
DE AltName: Full=Gene product 9 {ECO:0000305};
DE Short=gp9;
GN Name=9;
OS Enterobacteria phage T4 (Bacteriophage T4).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX NCBI_TaxID=10665;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=D;
RX PubMed=2726468; DOI=10.1093/nar/17.8.3303;
RA Prilipov A.G., Selivanov N.A., Efimov V.P., Marusich E.I.,
RA Mesyanzhinov V.V.;
RT "Nucleotide sequences of bacteriophage T4 genes 9, 10 and 11.";
RL Nucleic Acids Res. 17:3303-3303(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT "Bacteriophage T4 genome.";
RL Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=2403438; DOI=10.1128/jvi.64.1.143-154.1990;
RA Watts N.R., Coombs D.H.;
RT "Structure of the bacteriophage T4 baseplate as determined by chemical
RT cross-linking.";
RL J. Virol. 64:143-154(1990).
RN [4]
RP REVIEW.
RX PubMed=14625682; DOI=10.1007/s00018-003-3072-1;
RA Leiman P.G., Kanamaru S., Mesyanzhinov V.V., Arisaka F., Rossmann M.G.;
RT "Structure and morphogenesis of bacteriophage T4.";
RL Cell. Mol. Life Sci. 60:2356-2370(2003).
RN [5]
RP REVIEW ON FUNCTION.
RX PubMed=21129200; DOI=10.1186/1743-422x-7-355;
RA Leiman P.G., Arisaka F., van Raaij M.J., Kostyuchenko V.A., Aksyuk A.A.,
RA Kanamaru S., Rossmann M.G.;
RT "Morphogenesis of the T4 tail and tail fibers.";
RL Virol. J. 7:355-355(2010).
RN [6]
RP SUBUNIT.
RX PubMed=19896486; DOI=10.1016/j.jmb.2009.10.071;
RA Yap M.L., Mio K., Leiman P.G., Kanamaru S., Arisaka F.;
RT "The baseplate wedges of bacteriophage T4 spontaneously assemble into
RT hubless baseplate-like structure in vitro.";
RL J. Mol. Biol. 395:349-360(2010).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), FUNCTION, SUBUNIT, DOMAIN, AND
RP COILED COIL.
RX PubMed=10545330; DOI=10.1016/s0969-2126(00)80055-6;
RA Kostyuchenko V.A., Navruzbekov G.A., Kurochkina L.P., Strelkov S.V.,
RA Mesyanzhinov V.V., Rossmann M.G.;
RT "The structure of bacteriophage T4 gene product 9: the trigger for tail
RT contraction.";
RL Structure 7:1213-1222(1999).
RN [8]
RP STRUCTURE BY ELECTRON MICROSCOPY (17.0 ANGSTROMS) OF THE CONTRACTED TAIL,
RP AND SUBCELLULAR LOCATION.
RX PubMed=15315755; DOI=10.1016/j.cell.2004.07.022;
RA Leiman P.G., Chipman P.R., Kostyuchenko V.A., Mesyanzhinov V.V.,
RA Rossmann M.G.;
RT "Three-dimensional rearrangement of proteins in the tail of bacteriophage
RT T4 on infection of its host.";
RL Cell 118:419-429(2004).
RN [9]
RP STRUCTURE BY ELECTRON MICROSCOPY (4.11 ANGSTROMS), SUBUNIT, SUBCELLULAR
RP LOCATION, FUNCTION, AND IDENTIFICATION IN THE TAIL FIBER NETWORK.
RX PubMed=27193680; DOI=10.1038/nature17971;
RA Taylor N.M., Prokhorov N.S., Guerrero-Ferreira R.C., Shneider M.M.,
RA Browning C., Goldie K.N., Stahlberg H., Leiman P.G.;
RT "Structure of the T4 baseplate and its function in triggering sheath
RT contraction.";
RL Nature 533:346-352(2016).
CC -!- FUNCTION: Peripheral baseplate protein that is part of the tail fiber
CC network (PubMed:10545330, PubMed:27193680). Connects the long tail
CC fibers to the baseplate and, after virus attachment to a host cell,
CC probably changes its conformation to trigger the signal for tail
CC contraction (PubMed:10545330). Involved in the tail assembly
CC (PubMed:21129200). {ECO:0000269|PubMed:10545330,
CC ECO:0000269|PubMed:27193680, ECO:0000303|PubMed:21129200}.
CC -!- SUBUNIT: Homotrimer (PubMed:27193680). The gp9 trimer interacts with
CC the long tail fiber (LTF) that comprises gp34 trimer, gp35, gp36 and a
CC gp37 trimer. Part of the baseplate macromolecular complex which
CC consists of gp5, gp5.4, gp27 (central spike complex); gp6, gp25, gp53
CC (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11,
CC gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).
CC {ECO:0000269|PubMed:10545330, ECO:0000269|PubMed:19896486,
CC ECO:0000269|PubMed:27193680}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:15315755,
CC ECO:0000269|PubMed:2403438, ECO:0000269|PubMed:27193680}. Note=Present
CC in 18 copies in the baseplate. {ECO:0000303|PubMed:21129200}.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC {ECO:0000305}.
CC -!- DOMAIN: The C-terminus is bound to the baseplate, and the N-terminus
CC coiled-coil domain is associated with the long tail fibers.
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DR EMBL; X14192; CAA32395.1; -; Genomic_DNA.
DR EMBL; AF158101; AAD42520.1; -; Genomic_DNA.
DR PIR; S04082; GNBPT4.
DR RefSeq; NP_049767.1; NC_000866.4.
DR PDB; 1PDP; EM; 12.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R=1-288.
DR PDB; 1QEX; X-ray; 2.30 A; A/B=1-288.
DR PDB; 1S2E; X-ray; 2.30 A; A/B=1-288.
DR PDB; 1TJA; EM; 16.00 A; C/D/E=1-288.
DR PDB; 1ZKU; EM; 15.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R=1-288.
DR PDB; 5IV5; EM; 4.11 A; AA/AB/CC/CD/CE/EF/EG/EH/F/G/GI/GJ/H/HA/c/d/e/z=1-288.
DR PDB; 5IV7; EM; 6.77 A; AB/AC/AD/CD/CE/CF/EF/EG/F/FA/G/H/V/W/X/l/m/n=1-288.
DR PDBsum; 1PDP; -.
DR PDBsum; 1QEX; -.
DR PDBsum; 1S2E; -.
DR PDBsum; 1TJA; -.
DR PDBsum; 1ZKU; -.
DR PDBsum; 5IV5; -.
DR PDBsum; 5IV7; -.
DR SMR; P10927; -.
DR TCDB; 1.K.1.1.1; the gp27/5 t4-baseplate (t4-bp) family.
DR GeneID; 1258617; -.
DR KEGG; vg:1258617; -.
DR EvolutionaryTrace; P10927; -.
DR Proteomes; UP000009087; Genome.
DR GO; GO:0098025; C:virus tail, baseplate; IDA:UniProtKB.
DR GO; GO:0019076; P:viral release from host cell; IEA:InterPro.
DR GO; GO:0098003; P:viral tail assembly; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.640; -; 1.
DR Gene3D; 2.60.40.1680; -; 1.
DR InterPro; IPR008987; Baseplate_struct_prot_Gp9/10.
DR InterPro; IPR036240; Gp9-like_sf.
DR InterPro; IPR027411; Gp9/Gp10_mid_dom_sf.
DR InterPro; IPR027412; Gp9_C_dom_sf.
DR Pfam; PF07880; T4_gp9_10; 1.
DR SUPFAM; SSF50017; SSF50017; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Reference proteome; Viral baseplate protein;
KW Viral release from host cell; Viral tail assembly; Viral tail protein;
KW Virion.
FT CHAIN 1..288
FT /note="Baseplate protein gp9"
FT /id="PRO_0000164999"
FT COILED 20..80
FT /evidence="ECO:0000269|PubMed:10545330"
FT STRAND 15..19
FT /evidence="ECO:0007829|PDB:1QEX"
FT HELIX 20..39
FT /evidence="ECO:0007829|PDB:1QEX"
FT HELIX 45..49
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 50..53
FT /evidence="ECO:0007829|PDB:1QEX"
FT HELIX 74..76
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 84..86
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 90..92
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 94..98
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 106..110
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 118..120
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 122..130
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 134..139
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 144..152
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 154..156
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 159..169
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 176..180
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 185..193
FT /evidence="ECO:0007829|PDB:1QEX"
FT TURN 194..196
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 198..208
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 214..224
FT /evidence="ECO:0007829|PDB:1QEX"
FT TURN 225..228
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 229..241
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 243..247
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 251..257
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 260..266
FT /evidence="ECO:0007829|PDB:1QEX"
FT STRAND 268..282
FT /evidence="ECO:0007829|PDB:1QEX"
SQ SEQUENCE 288 AA; 30997 MW; 8028FCEBA25BB760 CRC64;
MFIQEPKKLI DTGEIGNAST GDILFDGGNK INSDFNAIYN AFGDQRKMAV ANGTGADGQI
IHATGYYQKH SITEYATPVK VGTRHDIDTS TVGVKVIIER GELGDCVEFI NSNGSISVTN
PLTIQAIDSI KGVSGNLVVT SPYSKVTLRC ISSDNSTSVW NYSIESMFGQ KESPAEGTWN
ISTSGSVDIP LFHRTEYNMA KLLVTCQSVD GRKIKTAEIN ILVDTVNSEV ISSEYAVMRV
GNETEEDEIA NIAFSIKENY VTATISSSTV GMRAAVKVIA TQKIGVAQ
