ID   T10B_DANRE              Reviewed;         202 AA.
AC   Q568N4;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit Tim10 B;
DE   AltName: Full=Mitochondrial import inner membrane translocase subunit Tim9 B;
DE   AltName: Full=TIMM10B;
DE            Short=Tim10b;
GN   Name=timm10b; Synonyms=fxc1, tim9b, timm9b; ORFNames=zgc:110193;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the TIM22 complex, a complex that mediates the
CC       import and insertion of multi-pass transmembrane proteins into the
CC       mitochondrial inner membrane. The TIM22 complex forms a twin-pore
CC       translocase that uses the membrane potential as the external driving
CC       force. {ECO:0000250|UniProtKB:Q9Y5J6}.
CC   -!- SUBUNIT: Component of the TIM22 complex.
CC       {ECO:0000250|UniProtKB:Q9Y5J6}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q9Y5J6}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q9Y5J6}.
CC   -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC       2 disulfide bonds in the mitochondrial intermembrane space. However,
CC       during the transit of timm10b from cytoplasm into mitochondrion, the
CC       Cys residues probably coordinate zinc, thereby preventing folding and
CC       allowing its transfer across mitochondrial outer membrane.
CC       {ECO:0000250|UniProtKB:P87108}.
CC   -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
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DR   EMBL; BC092787; AAH92787.1; -; mRNA.
DR   RefSeq; NP_001017829.1; NM_001017829.1.
DR   AlphaFoldDB; Q568N4; -.
DR   SMR; Q568N4; -.
DR   STRING; 7955.ENSDARP00000064462; -.
DR   PaxDb; Q568N4; -.
DR   GeneID; 550527; -.
DR   KEGG; dre:550527; -.
DR   CTD; 26515; -.
DR   ZFIN; ZDB-GENE-050417-370; timm10b.
DR   eggNOG; KOG3479; Eukaryota.
DR   InParanoid; Q568N4; -.
DR   OrthoDB; 1627953at2759; -.
DR   Reactome; R-DRE-1268020; Mitochondrial protein import.
DR   PRO; PR:Q568N4; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0042719; C:mitochondrial intermembrane space protein transporter complex; IBA:GO_Central.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.287.810; -; 1.
DR   InterPro; IPR004217; Tim10-like.
DR   InterPro; IPR035427; Tim10-like_dom_sf.
DR   Pfam; PF02953; zf-Tim10_DDP; 1.
DR   SUPFAM; SSF144122; SSF144122; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW   Mitochondrion inner membrane; Protein transport; Reference proteome;
KW   Translocation; Transport; Zinc.
FT   CHAIN           1..202
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit Tim10 B"
FT                   /id="PRO_0000228051"
FT   REGION          178..202
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           24..48
FT                   /note="Twin CX3C motif"
FT   DISULFID        24..48
FT                   /evidence="ECO:0000250|UniProtKB:P87108"
FT   DISULFID        28..44
FT                   /evidence="ECO:0000250|UniProtKB:P87108"
SQ   SEQUENCE   202 AA;  21861 MW;  BADB989B4D5337CA CRC64;
     MDPQGQLRNL RDFLLVYNRM TEICFHRCSS NFNYRNLTMD EERCVDSCAG KLIRTNHRLM
     GTYVQLMPAM VQKRMQEMES KAAEMAKAEA EAAAKAGTSV PDNPSSAITN ASMMSPSIQD
     IPNTSSTAPN GFQILSSPDN TSDIKTSSMT NGSAFGETNL HVITNDAKTF TNQSLDELIT
     PSPVNTSVPE TPTAGGAGQT PS