T10B_DROME
ID T10B_DROME Reviewed; 117 AA.
AC Q9Y0V3; A4V4S9;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit Tim10B;
DE AltName: Full=Mitochondrial import inner membrane translocase subunit Tim9B;
DE AltName: Full=Tim10b;
GN Name=Tim9b; Synonyms=tim9; ORFNames=CG17767;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10611480; DOI=10.1016/s0014-5793(99)01665-8;
RA Bauer M.F., Rothbauer U., Muehlenbein N., Smith R.J.H., Gerbitz K.-D.,
RA Neupert W., Brunner M., Hofmann S.;
RT "The mitochondrial TIM22 preprotein translocase is highly conserved
RT throughout the eukaryotic kingdom.";
RL FEBS Lett. 464:41-47(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Component of the TIM22 complex, a complex that mediates the
CC import and insertion of multi-pass transmembrane proteins into the
CC mitochondrial inner membrane. The TIM22 complex forms a twin-pore
CC translocase that uses the membrane potential as the external driving
CC force. In the TIM22 complex, it may act as a docking point for the
CC soluble 70 kDa complex that guides the target proteins in transit
CC through the aqueous mitochondrial intermembrane space (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC Tim22, associated with peripheral protein Tim9b/Tim10b and the 70 kDa
CC heterohexamer. In most cases, the 70 kDa complex is composed of TIMM9
CC and TIMM10 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}.
CC -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC 2 disulfide bonds in the mitochondrial intermembrane space. However,
CC during the transit of Tim9b/Tim10b from the cytoplasm into the
CC mitochondrion, the Cys residues probably coordinate zinc, thereby
CC preventing folding and allowing its transfer across the mitochondrial
CC outer membrane (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
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DR EMBL; AF150104; AAD40010.1; -; mRNA.
DR EMBL; AE014298; AAF48984.2; -; Genomic_DNA.
DR EMBL; AE014298; AAO41712.1; -; Genomic_DNA.
DR RefSeq; NP_001027074.1; NM_001031903.2.
DR RefSeq; NP_001027075.1; NM_001031904.3.
DR AlphaFoldDB; Q9Y0V3; -.
DR SMR; Q9Y0V3; -.
DR STRING; 7227.FBpp0074536; -.
DR PaxDb; Q9Y0V3; -.
DR DNASU; 3772213; -.
DR EnsemblMetazoa; FBtr0074767; FBpp0074536; FBgn0027358.
DR EnsemblMetazoa; FBtr0344072; FBpp0310503; FBgn0027358.
DR GeneID; 3772213; -.
DR KEGG; dme:Dmel_CG17767; -.
DR UCSC; CG17767-RB; d. melanogaster.
DR CTD; 3772213; -.
DR FlyBase; FBgn0027358; Tim9b.
DR VEuPathDB; VectorBase:FBgn0027358; -.
DR eggNOG; KOG3479; Eukaryota.
DR GeneTree; ENSGT00450000040326; -.
DR HOGENOM; CLU_141397_2_1_1; -.
DR InParanoid; Q9Y0V3; -.
DR OMA; CFNRCVD; -.
DR PhylomeDB; Q9Y0V3; -.
DR Reactome; R-DME-1268020; Mitochondrial protein import.
DR BioGRID-ORCS; 3772213; 1 hit in 1 CRISPR screen.
DR ChiTaRS; Tim9b; fly.
DR GenomeRNAi; 3772213; -.
DR PRO; PR:Q9Y0V3; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0027358; Expressed in testis and 11 other tissues.
DR Genevisible; Q9Y0V3; DM.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042719; C:mitochondrial intermembrane space protein transporter complex; ISS:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0140318; F:protein transporter activity; ISS:FlyBase.
DR GO; GO:0051082; F:unfolded protein binding; ISS:FlyBase.
DR GO; GO:0071456; P:cellular response to hypoxia; IMP:FlyBase.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; ISS:FlyBase.
DR Gene3D; 1.10.287.810; -; 1.
DR InterPro; IPR004217; Tim10-like.
DR InterPro; IPR035427; Tim10-like_dom_sf.
DR Pfam; PF02953; zf-Tim10_DDP; 1.
DR SUPFAM; SSF144122; SSF144122; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW Mitochondrion inner membrane; Protein transport; Reference proteome;
KW Translocation; Transport; Zinc.
FT CHAIN 1..117
FT /note="Mitochondrial import inner membrane translocase
FT subunit Tim10B"
FT /id="PRO_0000193604"
FT REGION 75..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 22..46
FT /note="Twin CX3C motif"
FT COMPBIAS 75..98
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 22..46
FT /evidence="ECO:0000250"
FT DISULFID 26..42
FT /evidence="ECO:0000250"
SQ SEQUENCE 117 AA; 13520 MW; 3327A332075A57E5 CRC64;
MDSNLRNLKD FFTLYNKVTE LCFSRCVDNL SQRDLGGHED LCVDRCVTKF ARFNQNMMKV
YVDVQTTINA KRMEEMEENA RKAEQQQREQ EKERLKEAAA TAVLTPVQPP VAGNLSM
