ID   T10B_RAT                Reviewed;         100 AA.
AC   Q9R1B1; O88471;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit Tim10 B;
DE   AltName: Full=Fracture callus protein 1;
DE   AltName: Full=FxC1;
DE   AltName: Full=Mitochondrial import inner membrane translocase subunit Tim9 B;
DE   AltName: Full=TIMM10B;
DE            Short=Tim10b;
GN   Name=Timm10b; Synonyms=Fxc1, Tim9b, Timm9b;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10611480; DOI=10.1016/s0014-5793(99)01665-8;
RA   Bauer M.F., Rothbauer U., Muehlenbein N., Smith R.J.H., Gerbitz K.-D.,
RA   Neupert W., Brunner M., Hofmann S.;
RT   "The mitochondrial TIM22 preprotein translocase is highly conserved
RT   throughout the eukaryotic kingdom.";
RL   FEBS Lett. 464:41-47(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 21-100.
RX   PubMed=9731230; DOI=10.1006/bbrc.1998.9167;
RA   Hadjiargyrou M., Halsey M.F., Ahrens W., Rightmire E.P., McLeod K.J.,
RA   Rubin C.T.;
RT   "Cloning of a novel cDNA expressed during the early stages of fracture
RT   healing.";
RL   Biochem. Biophys. Res. Commun. 249:879-884(1998).
CC   -!- FUNCTION: Component of the TIM22 complex, a complex that mediates the
CC       import and insertion of multi-pass transmembrane proteins into the
CC       mitochondrial inner membrane. The TIM22 complex forms a twin-pore
CC       translocase that uses the membrane potential as the external driving
CC       force. In the TIM22 complex, it may act as a docking point for the
CC       soluble 70 kDa complex that guides the target proteins in transit
CC       through the aqueous mitochondrial intermembrane space.
CC       {ECO:0000250|UniProtKB:Q9Y5J6}.
CC   -!- SUBUNIT: Component of the TIM22 complex, which core is composed of
CC       TIMM22, associated with TIMM10 (TIMM10A and/or TIMM10B), TIMM9, AGK and
CC       TIMM29. {ECO:0000250|UniProtKB:Q9Y5J6}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q9Y5J6}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q9Y5J6}.
CC   -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC       2 disulfide bonds in the mitochondrial intermembrane space. However,
CC       during the transit of TIMM10B from cytoplasm into mitochondrion, the
CC       Cys residues probably coordinate zinc, thereby preventing folding and
CC       allowing its transfer across mitochondrial outer membrane.
CC       {ECO:0000250|UniProtKB:P87108}.
CC   -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
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DR   EMBL; AF150106; AAD40012.1; -; mRNA.
DR   EMBL; AF061242; AAC34297.1; -; mRNA.
DR   PIR; JE0277; JE0277.
DR   RefSeq; NP_445823.1; NM_053371.2.
DR   AlphaFoldDB; Q9R1B1; -.
DR   SMR; Q9R1B1; -.
DR   STRING; 10116.ENSRNOP00000064088; -.
DR   jPOST; Q9R1B1; -.
DR   PaxDb; Q9R1B1; -.
DR   PRIDE; Q9R1B1; -.
DR   Ensembl; ENSRNOT00000076631; ENSRNOP00000068264; ENSRNOG00000050846.
DR   GeneID; 84384; -.
DR   KEGG; rno:84384; -.
DR   UCSC; RGD:71097; rat.
DR   CTD; 26515; -.
DR   RGD; 71097; Timm10b.
DR   eggNOG; KOG3479; Eukaryota.
DR   GeneTree; ENSGT00450000040326; -.
DR   HOGENOM; CLU_141397_2_2_1; -.
DR   InParanoid; Q9R1B1; -.
DR   OrthoDB; 1627953at2759; -.
DR   PhylomeDB; Q9R1B1; -.
DR   TreeFam; TF106188; -.
DR   PRO; PR:Q9R1B1; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Bgee; ENSRNOG00000050846; Expressed in pancreas and 20 other tissues.
DR   ExpressionAtlas; Q9R1B1; baseline and differential.
DR   Genevisible; Q9R1B1; RN.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISO:RGD.
DR   GO; GO:0005758; C:mitochondrial intermembrane space; ISO:RGD.
DR   GO; GO:0042719; C:mitochondrial intermembrane space protein transporter complex; ISO:RGD.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.287.810; -; 1.
DR   InterPro; IPR004217; Tim10-like.
DR   InterPro; IPR035427; Tim10-like_dom_sf.
DR   Pfam; PF02953; zf-Tim10_DDP; 1.
DR   SUPFAM; SSF144122; SSF144122; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW   Mitochondrion inner membrane; Protein transport; Reference proteome;
KW   Translocation; Transport; Zinc.
FT   CHAIN           1..100
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit Tim10 B"
FT                   /id="PRO_0000193600"
FT   MOTIF           25..49
FT                   /note="Twin CX3C motif"
FT   DISULFID        25..49
FT                   /evidence="ECO:0000250|UniProtKB:P87108"
FT   DISULFID        29..45
FT                   /evidence="ECO:0000250|UniProtKB:P87108"
SQ   SEQUENCE   100 AA;  11351 MW;  B15DB33C3A1D1E18 CRC64;
     MEHQQQQLRN LRDFLLVYNR MTELCFQRCV PSLHHRALDA EEEACLHSCA GKLIHSNHRL
     MAAYVHLMPA LVQRRMADYE AASAVPGVPA EQPRDSPSGS