ID   T10B_XENLA              Reviewed;         125 AA.
AC   Q6GR66;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit Tim10 B;
DE   AltName: Full=Mitochondrial import inner membrane translocase subunit Tim9 B;
GN   Name=timm10b; Synonyms=fxc1, tim9b, timm9b;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the TIM22 complex, a complex that mediates the
CC       import and insertion of multi-pass transmembrane proteins into the
CC       mitochondrial inner membrane. The TIM22 complex forms a twin-pore
CC       translocase that uses the membrane potential as the external driving
CC       force. {ECO:0000250|UniProtKB:Q9Y5J6}.
CC   -!- SUBUNIT: Component of the TIM22 complex.
CC       {ECO:0000250|UniProtKB:Q9Y5J6}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q9Y5J6}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q9Y5J6}.
CC   -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC       2 disulfide bonds in the mitochondrial intermembrane space. However,
CC       during the transit of timm10b from cytoplasm into mitochondrion, the
CC       Cys residues probably coordinate zinc, thereby preventing folding and
CC       allowing its transfer across mitochondrial outer membrane.
CC       {ECO:0000250|UniProtKB:P87108}.
CC   -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
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DR   EMBL; BC071063; AAH71063.1; -; mRNA.
DR   RefSeq; NP_001085318.1; NM_001091849.1.
DR   AlphaFoldDB; Q6GR66; -.
DR   SMR; Q6GR66; -.
DR   GeneID; 443743; -.
DR   KEGG; xla:443743; -.
DR   CTD; 443743; -.
DR   Xenbase; XB-GENE-6251887; timm10b.S.
DR   OrthoDB; 1627953at2759; -.
DR   Proteomes; UP000186698; Chromosome 2S.
DR   Bgee; 443743; Expressed in oocyte and 19 other tissues.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.287.810; -; 1.
DR   InterPro; IPR004217; Tim10-like.
DR   InterPro; IPR035427; Tim10-like_dom_sf.
DR   Pfam; PF02953; zf-Tim10_DDP; 1.
DR   SUPFAM; SSF144122; SSF144122; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW   Mitochondrion inner membrane; Protein transport; Reference proteome;
KW   Translocation; Transport; Zinc.
FT   CHAIN           1..125
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit Tim10 B"
FT                   /id="PRO_0000228052"
FT   REGION          87..125
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           25..49
FT                   /note="Twin CX3C motif"
FT   COMPBIAS        101..118
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        25..49
FT                   /evidence="ECO:0000250|UniProtKB:P87108"
FT   DISULFID        29..45
FT                   /evidence="ECO:0000250|UniProtKB:P87108"
SQ   SEQUENCE   125 AA;  14038 MW;  11E3155204FC457D CRC64;
     MEGEQQQLRN LRDFLLVYNK MTELCFSRCA KNLNYRSVTM EEEQCLDSCA SKFIRANHRL
     MSAYVSLMPS VVQRRMAEYE GAAANVPPIE TEPSADHMPP VISSHSGNSP TNKQLDSVSD
     LPVGK