BP25_BPT4
ID BP25_BPT4 Reviewed; 132 AA.
AC P09425;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 23-FEB-2022, entry version 110.
DE RecName: Full=Baseplate wedge protein gp25 {ECO:0000305};
DE AltName: Full=Outer wedge of baseplate protein;
DE AltName: Full=Protein Gp25;
GN Name=25;
OS Enterobacteria phage T4 (Bacteriophage T4).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX NCBI_TaxID=10665;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=D;
RX PubMed=3186452; DOI=10.1093/nar/16.20.9862;
RA Gruidl M.E., Canan N., Mosig G.;
RT "Bacteriophage T4 gene 25.";
RL Nucleic Acids Res. 16:9862-9862(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1871975; DOI=10.1016/0042-6822(91)90852-3;
RA Gruidl M.E., Chen T.C., Gargano S., Storlazzi A., Cascino A., Mosig G.;
RT "Two bacteriophage T4 base plate genes (25 and 26) and the DNA repair gene
RT uvsY belong to spatially and temporally overlapping transcription units.";
RL Virology 184:359-369(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT "Bacteriophage T4 genome.";
RL Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-132.
RX PubMed=3026891; DOI=10.1093/genetics/114.4.1061;
RA Gruidl M.E., Mosig G.;
RT "Sequence and transcripts of the bacteriophage T4 DNA repair gene uvsY.";
RL Genetics 114:1061-1079(1986).
RN [5]
RP PROTEIN SEQUENCE OF 2-11, AND SUBCELLULAR LOCATION.
RX PubMed=15342608; DOI=10.1128/jb.186.18.6335-6339.2004;
RA Ye N., Nemoto N.;
RT "Processing of the tail lysozyme (gp5) of bacteriophage T4.";
RL J. Bacteriol. 186:6335-6339(2004).
RN [6]
RP SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=2403438; DOI=10.1128/jvi.64.1.143-154.1990;
RA Watts N.R., Coombs D.H.;
RT "Structure of the bacteriophage T4 baseplate as determined by chemical
RT cross-linking.";
RL J. Virol. 64:143-154(1990).
RN [7]
RP REVIEW.
RX PubMed=14625682; DOI=10.1007/s00018-003-3072-1;
RA Leiman P.G., Kanamaru S., Mesyanzhinov V.V., Arisaka F., Rossmann M.G.;
RT "Structure and morphogenesis of bacteriophage T4.";
RL Cell. Mol. Life Sci. 60:2356-2370(2003).
RN [8]
RP REVIEW ON FUNCTION.
RX PubMed=21129200; DOI=10.1186/1743-422x-7-355;
RA Leiman P.G., Arisaka F., van Raaij M.J., Kostyuchenko V.A., Aksyuk A.A.,
RA Kanamaru S., Rossmann M.G.;
RT "Morphogenesis of the T4 tail and tail fibers.";
RL Virol. J. 7:355-355(2010).
RN [9]
RP SUBUNIT.
RX PubMed=19896486; DOI=10.1016/j.jmb.2009.10.071;
RA Yap M.L., Mio K., Leiman P.G., Kanamaru S., Arisaka F.;
RT "The baseplate wedges of bacteriophage T4 spontaneously assemble into
RT hubless baseplate-like structure in vitro.";
RL J. Mol. Biol. 395:349-360(2010).
RN [10]
RP STRUCTURE BY ELECTRON MICROSCOPY (17.0 ANGSTROMS) OF THE CONTRACTED TAIL,
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=15315755; DOI=10.1016/j.cell.2004.07.022;
RA Leiman P.G., Chipman P.R., Kostyuchenko V.A., Mesyanzhinov V.V.,
RA Rossmann M.G.;
RT "Three-dimensional rearrangement of proteins in the tail of bacteriophage
RT T4 on infection of its host.";
RL Cell 118:419-429(2004).
RN [11]
RP STRUCTURE BY ELECTRON MICROSCOPY (4.11 ANGSTROMS), SUBUNIT, SUBCELLULAR
RP LOCATION, AND FUNCTION.
RX PubMed=27193680; DOI=10.1038/nature17971;
RA Taylor N.M., Prokhorov N.S., Guerrero-Ferreira R.C., Shneider M.M.,
RA Browning C., Goldie K.N., Stahlberg H., Leiman P.G.;
RT "Structure of the T4 baseplate and its function in triggering sheath
RT contraction.";
RL Nature 533:346-352(2016).
CC -!- FUNCTION: Baseplate protein that is located next to the tail tube
CC (inner baseplate). Involved in sheath assembly. The gp25-(gp6)2-gp7
CC module is involved in sheath contraction (PubMed:27193680).
CC {ECO:0000269|PubMed:15315755, ECO:0000269|PubMed:27193680}.
CC -!- SUBUNIT: Homodimer. Interacts with gp53 and with the (gp6)2-gp7
CC heterotrimeric molecule; The gp25-(gp6)2-gp7 module is involved in
CC sheath contraction (PubMed:27193680). Part of the baseplate
CC macromolecular complex which consists of gp5, gp5.4, gp27 (central
CC spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8
CC (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and
CC gp54 (proximal region of the tail tube). {ECO:0000269|PubMed:19896486,
CC ECO:0000269|PubMed:2403438, ECO:0000269|PubMed:27193680}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:15315755,
CC ECO:0000269|PubMed:15342608, ECO:0000269|PubMed:2403438,
CC ECO:0000269|PubMed:27193680}. Note=Present in 6 copies in the
CC baseplate. {ECO:0000303|PubMed:21129200}.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC {ECO:0000269|PubMed:3186452}.
CC -!- SIMILARITY: Belongs to the GpW/Gp25 family. {ECO:0000305}.
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DR EMBL; X05134; CAA28777.1; -; Genomic_DNA.
DR EMBL; M77695; AAA32549.1; -; Genomic_DNA.
DR EMBL; AF158101; AAD42433.1; -; Genomic_DNA.
DR EMBL; X04856; CAA28547.1; -; Genomic_DNA.
DR PIR; JF0054; GYBPT4.
DR RefSeq; NP_049800.1; NC_000866.4.
DR PDB; 5IV5; EM; 4.11 A; BD/DG/FJ/IC/T/q=1-132.
DR PDB; 5IV7; EM; 6.77 A; BD/DF/GA/O/e/u=1-132.
DR PDB; 5IW9; X-ray; 2.47 A; A/B=2-132.
DR PDBsum; 5IV5; -.
DR PDBsum; 5IV7; -.
DR PDBsum; 5IW9; -.
DR SMR; P09425; -.
DR TCDB; 1.K.1.1.1; the gp27/5 t4-baseplate (t4-bp) family.
DR GeneID; 1258633; -.
DR KEGG; vg:1258633; -.
DR Proteomes; UP000009087; Genome.
DR GO; GO:0098025; C:virus tail, baseplate; IDA:UniProtKB.
DR InterPro; IPR007048; IraD/Gp25-like.
DR Pfam; PF04965; GPW_gp25; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Late protein; Reference proteome;
KW Viral baseplate protein; Viral tail protein; Virion.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:15342608"
FT CHAIN 2..132
FT /note="Baseplate wedge protein gp25"
FT /id="PRO_0000165013"
FT HELIX 21..41
FT /evidence="ECO:0007829|PDB:5IW9"
FT HELIX 59..63
FT /evidence="ECO:0007829|PDB:5IW9"
FT HELIX 69..84
FT /evidence="ECO:0007829|PDB:5IW9"
FT STRAND 88..98
FT /evidence="ECO:0007829|PDB:5IW9"
FT TURN 99..102
FT /evidence="ECO:0007829|PDB:5IW9"
FT STRAND 103..114
FT /evidence="ECO:0007829|PDB:5IW9"
FT STRAND 120..126
FT /evidence="ECO:0007829|PDB:5IW9"
SQ SEQUENCE 132 AA; 15095 MW; 16C2803EE5B12B0B CRC64;
MANINKLYSD IDPEMKMDWN KDVSRSLGLR SIKNSLLGII TTRKGSRPFD PEFGCDLSDQ
LFENMTPLTA DTVERNIESA VRNYEPRIDK LAVNVIPVYD DYTLIVEIRF SVIDNPDDIE
QIKLQLASSN RV
