T132A_HUMAN
ID T132A_HUMAN Reviewed; 1023 AA.
AC Q24JP5; Q69YU7; Q86VZ8; Q86W97; Q9H8K3; Q9HCI9; Q9NWY0;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Transmembrane protein 132A;
DE AltName: Full=HSPA5-binding protein 1;
DE Flags: Precursor;
GN Name=TMEM132A; Synonyms=HSPA5BP1, KIAA1583;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Brain;
RX PubMed=10997877; DOI=10.1093/dnares/7.4.271;
RA Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XVIII. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 7:273-281(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 364-1023 (ISOFORM 1), AND VARIANT VAL-969.
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-560 (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 188-560 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP PHOSPHORYLATION AT SER-529.
RX PubMed=26091039; DOI=10.1016/j.cell.2015.05.028;
RA Tagliabracci V.S., Wiley S.E., Guo X., Kinch L.N., Durrant E., Wen J.,
RA Xiao J., Cui J., Nguyen K.B., Engel J.L., Coon J.J., Grishin N.,
RA Pinna L.A., Pagliarini D.J., Dixon J.E.;
RT "A single kinase generates the majority of the secreted phosphoproteome.";
RL Cell 161:1619-1632(2015).
CC -!- FUNCTION: May play a role in embryonic and postnatal development of the
CC brain. Increased resistance to cell death induced by serum starvation
CC in cultured cells. Regulates cAMP-induced GFAP gene expression via
CC STAT3 phosphorylation (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with HSPA5/GRP78. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC pass type I membrane protein {ECO:0000250}. Endoplasmic reticulum
CC membrane {ECO:0000250}; Single-pass type I membrane protein
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q24JP5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q24JP5-2; Sequence=VSP_025304;
CC Name=3;
CC IsoId=Q24JP5-3; Sequence=VSP_025302, VSP_025305, VSP_025306;
CC Name=4;
CC IsoId=Q24JP5-4; Sequence=VSP_025303, VSP_025304;
CC -!- SIMILARITY: Belongs to the TMEM132 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB13409.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAB14613.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB046803; BAB13409.1; ALT_INIT; mRNA.
DR EMBL; AK000546; BAA91245.1; -; mRNA.
DR EMBL; AK023577; BAB14613.1; ALT_INIT; mRNA.
DR EMBL; BC028106; AAH28106.1; -; mRNA.
DR EMBL; BC032059; AAH32059.1; -; mRNA.
DR EMBL; BC114569; AAI14570.1; -; mRNA.
DR EMBL; AL359557; CAH10668.2; -; mRNA.
DR CCDS; CCDS44618.1; -. [Q24JP5-1]
DR CCDS; CCDS7997.1; -. [Q24JP5-2]
DR RefSeq; NP_060340.2; NM_017870.3. [Q24JP5-2]
DR RefSeq; NP_821174.1; NM_178031.2. [Q24JP5-1]
DR AlphaFoldDB; Q24JP5; -.
DR BioGRID; 120309; 155.
DR IntAct; Q24JP5; 59.
DR STRING; 9606.ENSP00000005286; -.
DR TCDB; 8.A.143.1.1; the tmem132 (tmem132) family.
DR GlyGen; Q24JP5; 3 sites, 1 O-linked glycan (1 site).
DR iPTMnet; Q24JP5; -.
DR PhosphoSitePlus; Q24JP5; -.
DR BioMuta; TMEM132A; -.
DR DMDM; 121940967; -.
DR EPD; Q24JP5; -.
DR jPOST; Q24JP5; -.
DR MassIVE; Q24JP5; -.
DR MaxQB; Q24JP5; -.
DR PaxDb; Q24JP5; -.
DR PeptideAtlas; Q24JP5; -.
DR PRIDE; Q24JP5; -.
DR ProteomicsDB; 61259; -. [Q24JP5-1]
DR ProteomicsDB; 61260; -. [Q24JP5-2]
DR ProteomicsDB; 61261; -. [Q24JP5-3]
DR ProteomicsDB; 61262; -. [Q24JP5-4]
DR ABCD; Q24JP5; 4 sequenced antibodies.
DR Antibodypedia; 52906; 73 antibodies from 20 providers.
DR DNASU; 54972; -.
DR Ensembl; ENST00000005286.8; ENSP00000005286.4; ENSG00000006118.15. [Q24JP5-2]
DR Ensembl; ENST00000453848.7; ENSP00000405823.2; ENSG00000006118.15. [Q24JP5-1]
DR GeneID; 54972; -.
DR KEGG; hsa:54972; -.
DR MANE-Select; ENST00000453848.7; ENSP00000405823.2; NM_178031.3; NP_821174.1.
DR UCSC; uc001nqi.4; human. [Q24JP5-1]
DR CTD; 54972; -.
DR DisGeNET; 54972; -.
DR GeneCards; TMEM132A; -.
DR HGNC; HGNC:31092; TMEM132A.
DR HPA; ENSG00000006118; Group enriched (brain, choroid plexus).
DR MIM; 617363; gene.
DR neXtProt; NX_Q24JP5; -.
DR OpenTargets; ENSG00000006118; -.
DR PharmGKB; PA134886953; -.
DR VEuPathDB; HostDB:ENSG00000006118; -.
DR eggNOG; KOG4789; Eukaryota.
DR GeneTree; ENSGT00940000161414; -.
DR HOGENOM; CLU_009871_0_0_1; -.
DR InParanoid; Q24JP5; -.
DR OMA; GPCGPWL; -.
DR OrthoDB; 598074at2759; -.
DR PhylomeDB; Q24JP5; -.
DR TreeFam; TF314981; -.
DR PathwayCommons; Q24JP5; -.
DR Reactome; R-HSA-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR Reactome; R-HSA-8957275; Post-translational protein phosphorylation.
DR SignaLink; Q24JP5; -.
DR BioGRID-ORCS; 54972; 39 hits in 1073 CRISPR screens.
DR ChiTaRS; TMEM132A; human.
DR GenomeRNAi; 54972; -.
DR Pharos; Q24JP5; Tdark.
DR PRO; PR:Q24JP5; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q24JP5; protein.
DR Bgee; ENSG00000006118; Expressed in cortical plate and 139 other tissues.
DR ExpressionAtlas; Q24JP5; baseline and differential.
DR Genevisible; Q24JP5; HS.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR026307; TMEM132.
DR InterPro; IPR031436; TMEM132_C.
DR InterPro; IPR031437; TMEM132_M.
DR InterPro; IPR031435; TMEM132_N.
DR PANTHER; PTHR13388; PTHR13388; 1.
DR Pfam; PF16070; TMEM132; 1.
DR Pfam; PF15706; TMEM132D_C; 1.
DR Pfam; PF15705; TMEM132D_N; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Endoplasmic reticulum; Glycoprotein; Golgi apparatus;
KW Membrane; Phosphoprotein; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..1023
FT /note="Transmembrane protein 132A"
FT /id="PRO_0000287096"
FT TOPO_DOM 36..852
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 853..873
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 874..1023
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 212..246
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 512..533
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 611..916
FT /note="Binds to HSPA5/GRP78"
FT /evidence="ECO:0000250"
FT REGION 671..1023
FT /note="Confers cellular localization similar to full-length
FT form"
FT /evidence="ECO:0000250"
FT REGION 766..839
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 905..961
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 822..836
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 529
FT /note="Phosphoserine; by FAM20C"
FT /evidence="ECO:0000269|PubMed:26091039"
FT CARBOHYD 280
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..33
FT /note="MCARMAGRTTAAPRGPYGPWLCLLVALALDVVR -> MAPGSAPASSGAVII
FT SPSYASS (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:10997877"
FT /id="VSP_025302"
FT VAR_SEQ 46..295
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_025303"
FT VAR_SEQ 337
FT /note="D -> DS (in isoform 2 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_025304"
FT VAR_SEQ 405..423
FT /note="AEELVNTAPLTGVPQHVPV -> VRRPPSLPGKAGGQVPGAP (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:10997877"
FT /id="VSP_025305"
FT VAR_SEQ 424..1023
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:10997877"
FT /id="VSP_025306"
FT VARIANT 699
FT /note="R -> H (in dbSNP:rs524523)"
FT /id="VAR_032262"
FT VARIANT 969
FT /note="A -> V (in dbSNP:rs2469887)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_032263"
FT CONFLICT 10
FT /note="T -> R (in Ref. 2; BAA91245)"
FT /evidence="ECO:0000305"
FT CONFLICT 824
FT /note="E -> K (in Ref. 2; BAB14613)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1023 AA; 110110 MW; 464408B227D764C8 CRC64;
MCARMAGRTT AAPRGPYGPW LCLLVALALD VVRVDCGQAP LDPVYLPAAL ELLDAPEHFR
VQQVGHYPPA NSSLSSRSET FLLLQPWPRA QPLLRASYPP FATQQVVPPR VTEPHQRPVP
WDVRAVSVEA AVTPAEPYAR VLFHLKGQDW PPGSGSLPCA RLHATHPAGT AHQACRFQPS
LGACVVELEL PSHWFSQAST TRAELAYTLE PAAEGPGGCG SGEENDPGEQ ALPVGGVELR
PADPPQYQEV PLDEAVTLRV PDMPVRPGQL FSATLLLRHN FTASLLTLRI KVKKGLHVTA
ARPAQPTLWT AKLDRFKGSR HHTTLITCHR AGLTEPDSSP LELSEFLWVD FVVENSTGGG
VAVTRPVTWQ LEYPGQAPEA EKDKMVWEIL VSERDIRALI PLAKAEELVN TAPLTGVPQH
VPVRLVTVDG GGALVEVTEH VGCESANTQV LQVSEACDAV FVAGKESRGA RGVRVDFWWR
RLRASLRLTV WAPLLPLRIE LTDTTLEQVR GWRVPGPAEG PAEPAAEASD EAERRARGCH
LQYQRAGVRF LAPFAAHPLD GGRRLTHLLG PDWLLDVSHL VAPHARVLDS RVASLEGGRV
VVGREPGVTS IEVRSPLSDS ILGEQALAVT DDKVSVLELR VQPVMGISLT LSRGTAHPGE
VTATCWAQSA LPAPKQEVAL SLWLSFSDHT VAPAELYDRR DLGLSVSAEE PGAILPAEEQ
GAQLGVVVSG AGAEGLPLHV ALHPPEPCRR GRHRVPLASG TAWLGLPPAS TPAPALPSSP
AWSPPATEAT MGGKRQVAGS VGGNTGVRGK FERAEEEARK EETEAREEEE EEEEEMVPAP
QHVTELELGM YALLGVFCVA IFIFLVNGVV FVLRYQRKEP PDSATDPTSP QPHNWVWLGT
DQEELSRQLD RQSPGPPKGE GSCPCESGGG GEAPTLAPGP PGGTTSSSST LARKEAGGRR
KRVEFVTFAP APPAQSPEEP VGAPAVQSIL VAGEEDIRWV CEDMGLKDPE ELRNYMERIR
GSS
